Fucose-containing sulfated polysaccharides from brown macroalgae Lobophora variegata with antioxidant, anti-inflammatory, and antitumoral effects

Castro, Luiza Sheyla Evenni P. Will; Pinheiro, Thuane S.; Castro, Allisson Jhonatan Gomes; Dore, Celina Maria Pinto Guerra; Silva, Naissandra B. da; Alves, Monique Gabriela Chagas Faustino; Santos, Marilia S. Nascimento; Leite, Edda Lisboa

doi:10.1007/s10811-013-0217-y

Cited by 24 publications

(9 citation statements)

References 39 publications

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“…Previous studies have shown that a fraction of fucans from L variegata acted strongly in scanning hydroxyl radicals and superoxide in vitro, which showed the antioxidant activity of this fraction (Castro et al 2014). However, in this study, LV fucans showed little to contribute to scanning reactive species generated in vitro by incubation of HT-29 cells with H 2 O 2 .…”

Section: Discussioncontrasting

confidence: 64%

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A fucan of a brown seaweed and its antitumoral property on HT-29 and immunomodulatory activity in murine RAW 264.7 macrophage cell line

et al. 2017

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Polysaccharides from algae are also proper candidates with therapeutic properties and immunomodulatory and antitumor effects. The brown seaweed Lobophora variegata synthesizes different groups of anionic polysaccharides with several biological properties. Sulfated polysaccharides were obtained by delipidation of seaweed, proteolysis, and fractionation with different volumes of acetone. A fraction of sulfated polysaccharides, fucans or fucoidans, was extracted with 0.8 v acetone and named L. variegata (LV). This fucan was assessed in the inflammatory process in rats, antitumor action on human colon adenocarcinoma (HT-29 cells), apoptosis, and its effect on the cell cycle. LV fraction, a galactofucan, exhibited a high ratio of total sugar/sulfate (1.5) and a very low level of proteins. This polysaccharide showed an antiinflammatory effect on two models of inflammation induced by croton oil and oxazolone in rats. LV was analyzed in cellular proliferation of HT-29. We also demonstrated the cytotoxic action against this cell line and induction in the apoptosis and decreased the cell cycle in phases S and G 2 /M and the accumulation of cells in the G 1 phase. Our studies with LV showed a marked immunomodulatory action without and with lipopolysaccharide (LPS) on RAW 264.7 cells. Comparative studies of two fucans, LV and FV (Fucus vesiculosus), with different structures were assessed on viability in macrophages, RAW 264.7 cells. Both showed cytotoxicity in these cells. We observed high levels of nitric oxide (NO) production, interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α) when treated at 0.25, 0.5, and 1.0 mg mL −1 of LV. Data also suggest that LV has potential antitumor effects on HT-29 and antiinflammatory and immunomodulatory effects on RAW 264.7 cells.

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Section: Discussioncontrasting

confidence: 64%

“…Bands with the same absorption were found in other studies analyzing different fractions of the same species of alga. NMR spectra of the fucans were too complex to allow direct structure elucidation, probably due to structural heterogeneity (Rocha et al 2005;Medeiros et al 2008;Paiva et al 2011;Castro et al 2014;Castro et al 2016).…”

Section: Discussionmentioning

confidence: 99%

A fucan of a brown seaweed and its antitumoral property on HT-29 and immunomodulatory activity in murine RAW 264.7 macrophage cell line

et al. 2017

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“…Especially the higher fucose content was observed in EMSV and highest glucose content was detected in EMBC respectively. In previous publications indicated that the fucose rich hydrolysates highly attribute to their bioactivities especially antixodant and anti-inflammatory activities and Castro et al (2014) also reported the potential antioxidant and anti-inflammatory activities of fucose containing sulfated polysaccharide from Lobophora variegata (Castro et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Comparision of antioxidant and anti-inflammatory activities of enzyme assisted hydrolysate from Ecklonia maxima blades and stipe

Lee¹,

Je²,

Hwang³

et al. 2021

Fish Aquat Sci

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Marine brown seaweeds are a source of functional ingredients with various biological properties. They have been used in the food and functional food industries. Brown seaweeds are divided into three parts of blades, stipe, and root. Normally seaweed blades were used as raw materials for biological research. However, there are limited uses on stipes of Ecklonia maxima (E. maxima) depending on the physicochemical, nutritional, and biological properties. Besides, the comparative studies of two structures of E. maxima, blades and stipe didn't discover previously. This study aimed to compare the potent antioxidant and anti-inflammatory activities of the two structures of E. maxima, blades and stipe in vitro studies to increase the utilization of the two structures of E. maxima. The enzyme-assisted hydrolysate from E. maxima showed significant antioxidant and anti-inflammatory activities. Among them, celluclast-assisted hydrolysate from E. maxima blades (EMBC) and viscozyme-assisted hydrolysate from E. maxima stipe (EMSV) expressed significant protection on hydrogen peroxide-induced oxidative stress. Moreover, EMBC and EMSV treatment remarkably reduced nitric oxide production by downregulation of pro-inflammatory cytokine expressions in lipopolysaccharide-stimulated Raw 264.7 cells. Especially EMBC showed strong inhibition on pro-inflammatory cytokine production compared to EMSV. Taken together research findings suggest that EMBC and EMSV possessed potent antioxidant and anti-inflammatory properties and may be utilized as functional ingredients in the food and functional food sectors.

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“…In potential cytotoxic activity on a cultured human melanoma cancer cell line (Rocha et al 2007). Lobophorolide (2) also showed antineoplastic activity (IC 50 0.03 μg ml-1) on the human colon tumor cell line HCT-116 (Kubanek et al 2003), and sulfated polyscaccharides presented anti-tumoral effects on human colon adenocarcinoma cell line HT-29 (Castro et al 2014b). Several organic Lobophora extracts were active against P-388 lymphocytic leukemia and Ehrlich ascites tumor in mice (Kashiwagi et al 1980).…”

Section: Additional Pharmacological Bioactivitiesmentioning

confidence: 99%

Biological activities associated to the chemodiversity of the brown algae belonging to genus Lobophora (Dictyotales, Phaeophyceae)

et al. 2015

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Although Lobophora belongs to a marine algal family (Dictyotaceae) that produces a large array of secondary metabolites, it has received little attention compared to other genera, such as Dictyota, in terms of natural compounds isolation and characterization. However, metabolites produced by Lobophora species have been found to exhibit a wide array of bioactivities including pharmacological (e.g. antibacterial, antiviral, antioxidant, antitumoral), pesticidal, and ecological. This review aims to report the state-of-the-art of the natural products isolated from Lobophora species (Dictyotales, Phaeophyceae) and their associated bioactivities. All bioactivities documented in the literature are reported, therefore including studies for which pure active substances were described, as well as studies limited to extracts or fractions. From the early 1980s until today, 49 scientific works have been published on Lobophora chemistry and bioactivity, among which 40 have reported bioactivities. Only six studies, however, have identified, characterized and tested no less than 23 bioactive pure compounds (three C-21 polyunsaturated alcohols, three fatty-acids, a macrolactone, 11 polyketides, a few sulfated polysaccharides, three sulfolipids, a tocopherol derivative). The present review intends to raise awareness of chemists and biologists given the recent significant taxonomic progress of this brown algal genus, which holds a promising plethora of natural products yet to be discovered with ecological and pharmacological properties

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Fucose-containing sulfated polysaccharides from brown macroalgae Lobophora variegata with antioxidant, anti-inflammatory, and antitumoral effects

Cited by 24 publications

References 39 publications

A fucan of a brown seaweed and its antitumoral property on HT-29 and immunomodulatory activity in murine RAW 264.7 macrophage cell line

A fucan of a brown seaweed and its antitumoral property on HT-29 and immunomodulatory activity in murine RAW 264.7 macrophage cell line

Comparision of antioxidant and anti-inflammatory activities of enzyme assisted hydrolysate from Ecklonia maxima blades and stipe

Biological activities associated to the chemodiversity of the brown algae belonging to genus Lobophora (Dictyotales, Phaeophyceae)

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