Fucosyltransferase gene expression in goat endometrium during the estrous cycle and early pregnancy

Newton, G. R.; Lewis, Shaye; Avendano, J.; Williams, Ebonee; Ribeiro, F. R. B.; Nuti, L. C.; Foxworth, William B.; Ing, Nancy H.

doi:10.1016/j.theriogenology.2019.04.022

Cited by 5 publications

(4 citation statements)

References 66 publications

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“…Subsequently, the goats were slaughtered at the local slaughterhouse on day 16 of the estrus cycle (C16) or pregnancy (P16). For each animal, the uterus was quickly removed and transported to the laboratory in an icebox, and pregnancy was confirmed by the presence of apparently normal filamentous conceptuses during uterine flushing [ 55 ]. The uteri were opened longitudinally along the antimesometrial side.…”

Section: Methodsmentioning

confidence: 99%

Integrating Analysis to Identify Differential circRNAs Involved in Goat Endometrial Receptivity

Wang

Zang

et al. 2023

IJMS

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Endometrial receptivity is one of the main factors underlying a successful pregnancy, with reports substantiating the fact that suboptimal endometrial receptivity accounts for two-thirds of early implantation event failures. The association between circRNAs and endometrial receptivity in the goat remains unclear. This study aims to identify potential circRNAs and regulatory mechanisms related to goat endometrial receptivity. Therefore, the endometrial samples on day 16 of pregnancy and day 16 of the estrous cycle were analyzed using high-throughput RNA-seq and bioinformatics. The results show that 4666 circRNAs were identified, including 7 downregulated and 11 upregulated differentially expressed circRNAs (DE-circRNAs). Back-splicing and RNase R resistance verified the identified circRNAs. We predicted the competing endogenous RNA (ceRNA) regulatory mechanism and potential target genes of DE-circRNAs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of these predicted target genes suggest that DE-circRNAs were significantly involved in establishing endometrial receptivity. Furthermore, Sanger sequencing, qPCR, correlation analysis and Fluorescence in Situ Hybridization (FISH) show that circ_MYRF derived from the host gene myelin regulatory factor (MYRF) might regulate the expression of interferon stimulating gene 15 (ISG15), thereby promoting the formation of endometrial receptivity. These novel findings may contribute to a better understanding of the molecular mechanisms regulating endometrial receptivity and promoting the maternal recognition of pregnancy (MRP).

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Section: Methodsmentioning

confidence: 99%

Integrating Analysis to Identify Differential circRNAs Involved in Goat Endometrial Receptivity

Wang

Zang

et al. 2023

IJMS

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“…14190144). Pregnancy was confirmed by the presence of apparently normal filamentous conceptuses in uterine flushing [ 32 ].…”

Section: Methodsmentioning

confidence: 99%

Differential Expression Pattern of Goat Uterine Fluids Extracellular Vesicles miRNAs during Peri-Implantation

Xie

Liu

Zang

et al. 2021

Cells

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Early pregnancy failure occurs when a mature embryo attaches to an unreceptive endometrium. During the formation of a receptive endometrium, extracellular vesicles (EVs) of the uterine fluids (UFs) deliver regulatory molecules such as small RNAs to mediate intrauterine communication between the embryo and the endometrium. However, profiling of small RNAs in goat UFs’ EVs during pregnancy recognition (day 16) has not been carried out. In this study, EVs were isolated from UFs on day 16 of the estrous cycle or gestation. They were isolated by Optiprep™ Density G radient (ODG) and verified by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and Western blotting. Immunostaining demonstrated that CD63 was present both in the endometrial epithelium and glandular epithelium, and stain intensity was greater in the pregnant endometrium compared to the non-pregnant endometrium. Small RNA sequencing revealed that UFs’ EVs contained numerous sRNA families and a total of 106 differentially expressed miRNAs (DEMs). Additionally, 1867 target genes of the DEMs were obtained, and miRNA–mRNA interaction networks were constructed. GO and KEGG analysis showed that miRNAs were significantly associated with the formation of a receptive endometrium and embryo implantation. In addition, the fluorescence in situ hybridization assay (FISH) showed that chi-miR-451-5p was mainly expressed in stromal cells of the endometrium and a higher level was detected in the endometrial luminal epithelium in pregnant states. Moreover, the dual-luciferase reporter assay showed that chi-miR-451-5p directly binds to PSMB8 and may play an important role in the formation of a receptive endometrium and embryo implantation. In conclusion, these results reveal that UFs’ EVs contain various small RNAs that may be vital in the formation of a receptive endometrium and embryo implantation.

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“…Goats were slaughtered at the local slaughterhouse on day 16 of the estrous cycle (C16) (n = 3) or pregnancy (P16) (n = 3). Pregnancy was confirmed by the presence of apparently normal filamentous conceptuses in uterine flushing [20]. For each animal, the uterus was quickly removed and transported to the laboratory in an icebox, and then it was opened longitudinally along the antimesometrial side.…”

Section: Animal and Tissue Collectionmentioning

confidence: 99%

Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats

et al. 2021

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Endometrial receptivity represents one of the leading factors affecting the successful implantation of embryos during early pregnancy. However, the mechanism of microRNAs (miRNAs) to establish goat endometrial receptivity remains unclear. This study was intended to identify potential miRNAs and regulatory mechanisms associated with establishing endometrial receptivity through integrating bioinformatics analysis and experimental verification. MiRNA expression profiles were obtained by high-throughput sequencing, resulting in the detection of 33 differentially expressed miRNAs (DEMs), followed by their validation through quantitative RT-PCR. Furthermore, 10 potential transcription factors (TFs) and 1316 target genes of these DEMs were obtained, and the TF–miRNA and miRNA–mRNA interaction networks were constructed. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that these miRNAs were significantly linked to establishing endometrial receptivity. Moreover, the fluorescence in situ hybridization (FISH) analysis, dual-luciferase report assay, and immunohistochemistry (IHC) analysis corroborated that chi-miR-483 could directly bind to deltex E3 ubiquitin ligase 3L (DTX3L) to reduce its expression level. In conclusion, our findings contribute to a better understanding of molecular mechanisms regulating the endometrial receptivity of goats, and they provide a reference for improving embryo implantation efficiency.

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Fucosyltransferase gene expression in goat endometrium during the estrous cycle and early pregnancy

Cited by 5 publications

References 66 publications

Integrating Analysis to Identify Differential circRNAs Involved in Goat Endometrial Receptivity

Integrating Analysis to Identify Differential circRNAs Involved in Goat Endometrial Receptivity

Differential Expression Pattern of Goat Uterine Fluids Extracellular Vesicles miRNAs during Peri-Implantation

Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats

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