Fuel Ethanol Production from Lignocellulose Using Genetically Engineered Bacteria

Ingram, L. O.; Lai, Xiaokuang; Moniruzzaman, Mohammed; Wood, B. E.; York, Sean W.

doi:10.1021/bk-1997-0666.ch003

Cited by 22 publications

(16 citation statements)

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“…It also hydrolyzes some hemicellulose components to syrup of monomeric sugars, exposes cellulose to enzymatic digestion, and solubilizes heavy metals that may be contaminating feedstocks (Ingram et al 1997;Lavarack et al 2002).…”

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confidence: 99%

Pretreatment and Enzymatic Hydrolysis of Sorghum Bran

2007

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Cereal Chem. 84(1):61-66Sorghum bran has potential to serve as a low-cost feedstock for production of fuel ethanol. Sorghum bran from a decortication process (10%) was used for this study. The approximate chemical composition of sorghum bran was 30% starch, 18% hemicellulose, 11% cellulose, 11% protein, 10% crude fat, and 3% ash. The objective of this research was to evaluate the effectiveness of selected pretreatment methods such as hot water, starch degradation, dilute acid hydrolysis, and combination of those methods on enzymatic hydrolysis of sorghum bran. Methods for pretreatment and enzymatic hydrolysis of sorghum bran involved hot water treatment (10% solid, w/v) at 130°C for 20 min, acid hydrolysis (H 2 SO 4 ), starch degradation, and enzymatic hydrolysis (60 hr, 50°C, 0.9%, v/v) with commercial cellulase and hemicellulose enzymes. Total sugar yield by using enzymatic hydrolysis alone was 9%, obtained from 60 hr of enzyme hydrolysis. Hot water treatment facilitated and increased access of the enzymes to hemicellulose and cellulose, improving total sugar yield up to 34%. Using a combination of starch degradation, optimum hot water treatment, and optimum enzymatic hydrolysis resulted in maximum total sugar yield of up to 75%.

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confidence: 99%

Pretreatment and Enzymatic Hydrolysis of Sorghum Bran

2007

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“…These enzymes are then added to the pretreated solids to release most of the sugars left in the solids, and an organism can be added to the same vessel to ferment the sugars released to ethanol in a confi guration known as the simultaneous saccharifi cation and fermentation (SSF) process, an approach that enhances rates, yields, and concentrations by reducing inhibition by the sugars released and also lowers containment costs (Spindler et al 1991 ;Wyman et al 1992 ;Katzen and Fowler 1994 ;Ingram and Zhou 2002 ). Sugars released in pretreatment, mostly from hemicellulose, are fermented to ethanol with an organism that has been genetically modifi ed to achieve high yields from the fi ve carbon sugars arabinose and xylose that native organisms could not effectively ferment to ethanol (Ho and Tsao 1995 ;Zhang et al 1995 ;Ingram et al 1997 ). The hemicellulose sugar stream could also be left with the pretreated solids and fermented to ethanol in the same vessel in a confi guration known as simultaneous saccharifi cation and co -fermentation (SSCF).…”

Section: Biological Processing Of Cellulosic Biomass To Ethanolmentioning

confidence: 99%

Cellulosic Ethanol from Agricultural Residues

Yang

Wyman

2010

Biofuels From Agricultural Wastes and Byproducts

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“…Engineering of the Z. mobilis ethanol pathway in E. coli has been previously reviewed (Dien et al, 2003;Ingram et al, 1997Ingram et al, , 1999Lin and Tanaka, 2006;Zaldivar et al, 2001). Briefly, pyruvate decarboxylase (PDC) and alcohol dehydrogenase II (ADH II) genes from Z. mobilis were integrated into the chromosome of E. coli to generate E. coli strain K011.…”

Section: Ethanol (Ch 3 Ch 2 Oh; C 2 H 6 O)mentioning

confidence: 99%

Microbial conversion of sugars from plant biomass to lactic acid or ethanol

2008

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SummaryConcerns for our environment and unease with our dependence on foreign oil have renewed interest in converting plant biomass into fuels and 'green' chemicals. The volume of plant matter available makes lignocellulose conversion desirable, although no single isolated organism has been shown to depolymerize lignocellulose and efficiently metabolize the resulting sugars into a specific product. This work reviews selected chemicals and fuels that can be produced from microbial fermentation of plant-derived cell-wall sugars and directed engineering for improvement of microbial biocatalysts. Lactic acid and ethanol production are highlighted, with a focus on engineered Escherichia coli.

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Fuel Ethanol Production from Lignocellulose Using Genetically Engineered Bacteria

Cited by 22 publications

References 0 publications

Pretreatment and Enzymatic Hydrolysis of Sorghum Bran

Pretreatment and Enzymatic Hydrolysis of Sorghum Bran

Cellulosic Ethanol from Agricultural Residues

Microbial conversion of sugars from plant biomass to lactic acid or ethanol

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