Fuel ethanol production from steam-pretreated corn stover using SSF at higher dry matter content

Öhgren, Karin; Rudolf, Andreas; Galbe, Mats; Zacchi, Guido

doi:10.1016/j.biombioe.2006.02.002

Cited by 193 publications

(79 citation statements)

References 13 publications

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“…The particle size of rice straw and silvergrass inversely influenced the acetic acid formation in dilute acid pretreatment (Guo et al, 2008); however, it was not confirmed in this study. The acetic acid concentration was lower than dilute acid Hodge et al, 2008), formic acid (Xu et al, 2009a), lime , alkali ), hydrothermal pretreatment (Öhgren et al, 2006;Lu et al, 2010) of corn stover; compared to other feedstocks listed in the table. NaOH has higher reactivity with hemicellulose than cellulose due to amorphous characteristics of hemicellulose (Lai, 2001).…”

Section: Byproducts Formationmentioning

confidence: 89%

Application of Response Surface Methodology to Optimize Alkali Concentration, Corn Stover Particle Size, and Extruder Parameters for Maximum Sugar Recovery

Karunanithy¹,

Muthukumarappan²

2011

Biofuel Production-Recent Developments and Prospects

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Section: Byproducts Formationmentioning

confidence: 89%

Application of Response Surface Methodology to Optimize Alkali Concentration, Corn Stover Particle Size, and Extruder Parameters for Maximum Sugar Recovery

Karunanithy¹,

Muthukumarappan²

2011

Biofuel Production-Recent Developments and Prospects

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“…O formulado ideal pode ser obtido pelo cocultivo de linhagens superprodutoras dos principais tipos de celulases; 95 pela produção em separado das celulases e posterior mistura dos extratos, em proporções pré-otimizadas; [92][93][94][96][97][98][99][100] ou ainda pela incorporação controlada de genes de celulases em organismos hospedeiros, de forma que a célula modificada já excrete as enzimas em proporções ideais. 61 No âmbito da produção de etanol a partir de materiais lignocelulósicos, as celulases são insumos que impactam significativamente o processo, podendo representar até 18% do custo operacional de uma planta.…”

Section: Tendências Futuras Para a Produção E Aplicação De Celulasesunclassified

Produção, propriedades e aplicação de celulases na hidrólise de resíduos agroindustriais

Castro¹,

Pereira²

2010

Quím. Nova

107

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Recebido em 13/5/09; aceito em 24/6/09; publicado na web em 25/11/09 PRODUCTION, PROPERTIES AND APPLICATION OF CELLULASES IN THE HYDROLYSIS OF AGROINDUSTRIAL RESIDUES. Cellulases have been intensively studied in the past few years, due to the interests in biofuels production from lignocellulosic materials, since they permit maintaining mild conditions during the conversion process. These enzymes can be produced by a broad variety of naturally occurring microorganisms, such as from genera Aspergillus, Trichoderma, Penicillium and Humicola. Targeting the increasing of expression levels, molecular biology tools have been used for heterologous genes insertion in host cells, e. g., Pichia pastoris and Escherichia coli. Enzymes from fungal cellulolytic complex usually act best at pH between 4 and 5 under temperatures from 40 to 60 °C and can be used for either sequential (SHF) or simultaneous (SSF) hydrolysis together alcoholic fermentation. In this review, the main raw materials for production of cellulases are identified, as well as the state of art of enzymes' properties, production and main applications.Keywords: cellulases; agroindustrial residues; lignocellulose. INTRODUÇÃOCelulases são enzimas que constituem um complexo capaz de atuar sobre materiais celulósicos, promovendo sua hidrólise. Estas enzimas são biocatalisadores altamente específicos que atuam em sinergia para a liberação de açúcares, dos quais glicose é o que desperta maior interesse industrial, devido à possibilidade de sua conversão em etanol. 1,2Estas enzimas começaram a ser estudadas durante a Segunda Guerra Mundial. A deterioração de fardas, barracas, bolsas e demais objetos dos acampamentos, fabricados de algodão, chamou a atenção de soldados das forças armadas norte-americanas, instalados nas ilhas Solomon, no Pacífico Sul. Algumas organizações, como a Quartermaster Corps, juntamente com as forças armadas, montaram laboratórios em busca de explicações e soluções imediatas para esse problema, que incluíam a detecção de organismos agentes das deteriorações, seus mecanismos de ação e métodos de controle. O grupo de trabalho, constituído por oito pesquisadores e liderado pelo Dr. Elwyn T. Reese, passou a conduzir seus experimentos no laboratório das forças armadas, em Natick, Massachusets, Estados Unidos. Como resultado das pesquisas, uma linhagem, codificada como QM6a, de um fungo filamentoso, identificado posteriormente como Trichoderma viride, foi isolada e a esta foi atribuída a característica de excretar enzimas capazes de degradar celulose. Até 1953, Dr. Reese e seu grupo de trabalho já haviam determinado que enzimas naturais, nomeadas celulases, constituem complexos de diversas moléculas com distintas habilidades na degradação do substrato. Em 1956, Dr. Reese aliou seus conhecimentos aos da Dra. Mary Mandels, que, em conjunto, passaram a trabalhar no laboratório em Natick. A partir de então, o foco das pesquisas deixou de ser a prevenção da hidrólise da celulose e passou a ser o melhoramento da produção das enzimas responsáveis por esse fe...

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“…However, SSF is constrained by the typically suboptimal conditions for hydrolysis and fermentation, and limited by the available carbon sources for the microbes. These problems are reduced by adding a pre-hydrolysis step into SSF, which allows the enzymatic phase to operate at optimal temperatures and yields more ethanol than other processes (Öhgren et al 2006;Ko et al 2009;Souza et al 2012;Tan et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Improved Ethanol Production Based on High Solids Fed-Batch Simultaneous Saccharification and Fermentation with Alkali-Pretreated Sugarcane Bagasse

Liu¹,

Xu²,

Zhang³

et al. 2016

BioResources

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Alkali-pretreated sugarcane bagasse fiber was subjected to fed-batch simultaneous saccharification and fermentation (SSF) with a prehydrolysis process to increase the solids loading and produce a high concentration of ethanol. The hydrolysis medium and yeast feeding modes were investigated to determine suitable conditions for high sugar yield and ethanol production. Batch addition resulted in a cumulative substrate concentration of up to 36% (w/v) and enhanced ethanol concentrations, while ethanol conversion efficiency gradually declined. Enzymatic prehydrolysis and fermentation with fed-batch mode contributed to the SSF process. The highest ethanol concentration was 66.915 g/L with the conversion efficiency of 72.89%, which was achieved at 30% (w/v) solids content after 96 h of fermentation. Hydrolyzed medium and yeast were added in batch mode at 24 h of enzymatic hydrolysis and fermentation, respectively. Thus, combining the fed-batch mode with pre-hydrolysis SSF produced a high yield of ethanol.

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Fuel ethanol production from steam-pretreated corn stover using SSF at higher dry matter content

Cited by 193 publications

References 13 publications

Application of Response Surface Methodology to Optimize Alkali Concentration, Corn Stover Particle Size, and Extruder Parameters for Maximum Sugar Recovery

Application of Response Surface Methodology to Optimize Alkali Concentration, Corn Stover Particle Size, and Extruder Parameters for Maximum Sugar Recovery

Produção, propriedades e aplicação de celulases na hidrólise de resíduos agroindustriais

Improved Ethanol Production Based on High Solids Fed-Batch Simultaneous Saccharification and Fermentation with Alkali-Pretreated Sugarcane Bagasse

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