Fuel‐Powered DNA Nanomachines for Biosensing and Cancer Therapy

“…One of the most dynamic and fast-developing areas of nucleic acid chemistry is DNA nanotechnology [1,2], which employs DNA molecules as a unique material for diverse 2D or 3D nanoarchitectures [3,4], which can be rationally designed and precisely controlled to furnish the components of dynamic DNA nanodevices [5][6][7], artificial nanocompartments [8], nanomachines [9,10], and nanorobots [11][12][13]. At the very foundation of this area of science lies the intrinsic propensity of polynucleotides to form double-helical complexes via complementary Watson-Crick base-pairing, as well as self-assemble into higher-order structures such as triplexes, G-quadruplexes, or i-motifs [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Template-Assisted Assembly of Hybrid DNA/RNA Nanostructures Using Branched Oligodeoxy- and Oligoribonucleotides

Fokina,

Poletaeva,

Dukova

et al. 2023

IJMS

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A template-assisted assembly approach to a C24 fullerene-like double-stranded DNA polyhedral shell is proposed. The assembly employed a supramolecular oligonucleotide dendrimer as a 3D template that was obtained via the hybridization of siRNA strands and a single-stranded DNA oligonucleotide joined to three- or four-way branched junctions. A four-way branched oligonucleotide building block (a starlet) was designed for the assembly of the shell composed of three identical self-complementary DNA single strands and a single RNA strand for hybridization to the DNA oligonucleotides of the template. To prevent premature auto-hybridization of the self-complementary oligonucleotides in the starlet, a photolabile protecting group was introduced via the N3-substituted thymidine phosphoramidite. Cleavable linkers such as a disulfide linkage, RNase A sensitive triribonucleotides, and di- and trideoxynucleotides were incorporated into the starlet and template at specific points to guide the post-assembly disconnection of the shell from the template, and enzymatic disassembly of the template and the shell in biological media. At the same time, siRNA strands were modified with 2′-OMe ribonucleotides and phosphorothioate groups in certain positions to stabilize toward enzymatic digestion. We report herein a solid-phase synthesis of branched oligodeoxy and oligoribonucleotide building blocks for the DNA/RNA dendritic template and the branched DNA starlet for a template-assisted construction of a C24 fullerene-like DNA shell after initial molecular modeling, followed by the assembly of the shell around the DNA-coated RNA dendritic template, and visualization of the resulting nanostructure by transmission electron microscopy.

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Dynamic Addressing Molecular Robot (DAMR): An Effective and Efficient Trial‐and‐Error Approach for the Analysis of Single Nucleotide Polymorphisms

Chen,

Cao

et al. 2024

Advanced Science

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Accurate and efficient molecular recognition plays a crucial role in the fields of molecular detection and diagnostics. Conventional trial‐and‐error‐based molecular recognition approaches have always been challenged in distinguishing minimal differences between targets and non‐targets, such as single nucleotide polymorphisms (SNPs) of oligonucleotides. To address these challenges, here, a novel concept of dynamic addressing analysis is proposed. In this concept, by dissecting the regions of the target and creating a corresponding recognizer, it is possible to eliminate the inaccuracy and inefficiency of recognition. To achieve this concept, a Dynamic Addressing Molecular Robot (DAMR), a DNA‐based dynamic addressing device is developed which is capable of dynamically locating targets. DAMR is designed to first bind to the conserved region of the target while addressing the specific region dynamically until accurate recognition is achieved. DAMR has provided an approach for analyzing low‐resolution targets and has been used for analyzing SNP of miR‐196a2 in both cell and serum samples, which has opened new avenues for effective and efficient molecular recognition.

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Fuel‐Powered DNA Nanomachines for Biosensing and Cancer Therapy

Cited by 6 publications

References 147 publications

Advances in self-assembled Au-DNA nanomachines

Advances in self-assembled Au-DNA nanomachines

Template-Assisted Assembly of Hybrid DNA/RNA Nanostructures Using Branched Oligodeoxy- and Oligoribonucleotides

Dynamic Addressing Molecular Robot (DAMR): An Effective and Efficient Trial‐and‐Error Approach for the Analysis of Single Nucleotide Polymorphisms

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