Fufang Shenhua tablet inhibits renal fibrosis by inhibiting PI3K/AKT

Shi, Chunru; Wei, Cuiting; Wang, Chao; Du, Hongjian; Liu, Ran; Wang, Xu; Hong, Quan; Chen, Xiangmei

doi:10.1016/j.phymed.2023.154873

Cited by 14 publications

(4 citation statements)

References 26 publications

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“…As shown in Figure1A,B, vimentin expression was significantly upregulated after 48 h of TGFβ1 stimulation. This result is consistent with that reported in several studies, indicating the occurrence of EMT in HK-2 cells upon TGFβ1 stimulation [27][28][29]. Of note, the expression of vimentin phosphorylation at Ser72 was significantly increased after TGFβ1 stimulation, suggesting its potential role in EMT progression of HK-2 cells.…”

supporting

confidence: 92%

Phosphorylated vimentin at Ser72 is associated with epithelial–mesenchymal transition in lupus nephritis

Wang,

Zhang,

et al. 2023

Int J of Rheum Dis

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ObjectivesTo measure the expression of vimentin and its phosphorylated forms in lupus nephritis (LN) and explore their potential role in LN development.MethodsLupus renal biopsies from LN patients and normal renal biopsies from kidney transplant donors were collected. The expression of vimentin and its phosphorylated forms (p‐vimentin (Ser39, Ser56, Ser72, Ser83, and Tyr117)) were measured by Western blots and immunohistochemistry. To construct stable cell line that overexpress vimentin and its phosphorylated forms, an immortalized proximal tubule epithelial cell line (HK‐2 cells) was utilized. The roles of vimentin and its phosphorylated forms on the migration of HK‐2 cells were examined by transwell migration assay and wound healing analysis.ResultsWe first observed a significant upregulation of vimentin protein in TGFβ1‐induced HK‐2 cells. This finding was further confirmed in renal tissues obtained from LN patients and animal model. Interestingly, among the five phosphorylated forms of vimentin, only vimentin phosphorylated at Ser72 was upregulated in LN. Through the establishment of stable vimentin and its phosphorylated forms overexpression in HK‐2 cells, we found that the overexpression of vimentin and its phosphorylated forms at Ser72 significantly enhances the cell migration.ConclusionsVimentin phosphorylated on Ser72 is important for renal epithelial cell migration, which would enhance the progression of vimentin‐induced epithelial–mesenchymal transition during LN development.

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supporting

confidence: 92%

Phosphorylated vimentin at Ser72 is associated with epithelial–mesenchymal transition in lupus nephritis

Wang,

Zhang,

et al. 2023

Int J of Rheum Dis

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“…The studies by Peng and colleagues have shown that, in patients with CKD, quercetin can improve azotemia, reduce hyperuricemia, and relieve the inflammatory response, but it does not reduce serum creatinine levels in mice ( 45 ). Additionally, the Fufang Shenhua tablet, a traditional Chinese medicine (TCM) with quercetin as its major component, has been found to improve renal fibrosis ( 46 ). Abelmoschus manihot , a herbal flowering plant rich in quercetin used in TCM, primarily exerts its therapeutic effect by mitigating inflammatory responses, reducing oxidative stress, and improving renal fibrosis ( 23 ).…”

Section: Discussionmentioning

confidence: 99%

Association between consumption of flavonol and its subclasses and chronic kidney disease in US adults: an analysis based on National Health and Nutrition Examination Survey data from 2007–2008, 2009–2010, and 2017–2018

Liu,

Tang,

et al. 2024

Front. Nutr.

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BackgroundThere is little research on the relationship between flavonol consumption and chronic kidney disease (CKD). This study aimed to examine the link between flavonol consumption and the risk of CKD among US adults, using data from the 2007–2008, 2009–2010 and 2017–2018 National Health and Nutrition Examination Survey (NHANES).MethodsA cross-sectional approach was used, drawing on data from three NHANES cycles. The flavonol consumption of the participants in this study was assessed using a 48 h dietary recall interview. CKD was diagnosed based on an estimated glomerular filtration rate below 60 mL/min/1.73 m2 or a urine albumin-to-creatinine ratio of 30 mg/g or higher.ResultsCompared to the lowest quartile of flavonol intake (Q1), the odds ratios for CKD were 0.598 (95% CI: 0.349, 1.023) for the second quartile (Q2), 0.679 (95% CI: 0.404, 1.142) for the third quartile (Q3), and 0.628 (95% CI: 0.395, 0.998) for the fourth quartile (Q4), with a p value for trend significance of 0.190. In addition, there was a significant trend in CKD risk with isorhamnetin intake, with the odds ratios for CKD decreasing to 0.860 (95% CI: 0.546, 1.354) in the second quartile, 0.778 (95% CI: 0.515, 1.177) in the third quartile, and 0.637 (95% CI: 0.515, 1.177) in the fourth quartile (p for trend = 0.013).ConclusionOur analysis of the NHANES data spanning 2007–2008, 2009–2010, and 2017–2018 suggests that high consumption of dietary flavonol, especially isorhamnetin, might be linked to a lower risk of CKD in US adults. These findings offer new avenues for exploring strategies for managing CKD.

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“…Excessive and suppressed PI3K/AKT signaling has been linked to various pathologies. [ 43 , 44 ]. The activation of the PI3K/AKT pathway has been reported to induce the epithelial–mesenchymal transition and the proliferation of epithelial cells [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

miR–122–5p Promotes Cowshed Particulate Matter2.5-Induced Apoptosis in NR8383 by Targeting COL4A1

Sun,

et al. 2024

Toxics

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It is well known that Particulate Matter2.5 (PM2.5) has a major adverse effect on the organism. However, the health hazards of livestock farm PM2.5 to humans and animals are not yet known, and the role of miRNAs in the cellular damage induced by livestock farm PM2.5 is also unclear. Therefore, our study used cowshed PM2.5 to stimulate rat alveolar macrophage NR8383 to construct an in vitro injury model to investigate the effect of miR−122−5p on PM2.5-induced apoptosis in the NR8383. The level of apoptosis was quantified by flow cytometry and Hoechst 33342/PI double staining. Furthermore, the potential target gene Collagen type IV alpha (COL4A1) of miR−122−5p was identified through the use of bioinformatics methods. The results demonstrated a decline in cell viability and an increase in apoptosis with rising PM2.5 concentrations and exposure durations. The transfection of miR−122−5p mimics resulted in an upregulation of the pro-apoptotic protein Bcl−xL/Bcl−2 and activation of cleaved caspase−3 while inhibiting the anti-apoptotic protein B−cell lymphoma−2. The experimental data indicate that miR−122−5p is involved in the apoptotic process by targeting COL4A1. Furthermore, the overexpression of COL4A1 was observed to enhance the PM2.5-activated PI3K/AKT/NF−κB signaling pathway, which contributed to the inhibition of apoptosis. This finding offers a promising avenue for the development of therapeutic strategies aimed at mitigating cellular damage induced by PM2.5 exposure.

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Fufang Shenhua tablet inhibits renal fibrosis by inhibiting PI3K/AKT

Cited by 14 publications

References 26 publications

Phosphorylated vimentin at Ser72 is associated with epithelial–mesenchymal transition in lupus nephritis

Phosphorylated vimentin at Ser72 is associated with epithelial–mesenchymal transition in lupus nephritis

Association between consumption of flavonol and its subclasses and chronic kidney disease in US adults: an analysis based on National Health and Nutrition Examination Survey data from 2007–2008, 2009–2010, and 2017–2018

miR–122–5p Promotes Cowshed Particulate Matter2.5-Induced Apoptosis in NR8383 by Targeting COL4A1

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