Fuji surge technique and continuous in‐line filtration to improve the quality of single donor platelet concentrates

Abstract

White blood cell (WBC)-reduced single donor platelet concentrates (SDPs) can be collected by most cell separators. WBC reduction can be achieved directly during plateletpheresis or by filtration. Continuous filtration with low filtration rates provides SDPs of good purity. To compensate the platelet (PLT) loss due to filtration, the PLT yield in the unfiltered primary product should be optimal. Fifty donors underwent plateletpheresis with the MCS+ blood cell separator (Haemonetics) with the new Fuji surge technique and continuous WBC filtration. Twelve SDPs were analysed for PLT yield, red blood cells (RBC), WBC, and pH after collection (Day 0) and at the end of storage (Day 5). Thereafter, further 38 SDPs were measured for PLT and WBC content in routine production at Day 0. PLT were determined electronically, RBC and WBC were counted manually (Neubauer and Nageotte chamber, respectively). For pH measurement, a pH-meter was used. Mean blood volume processed was 2621 +/- 112 ml in a donation time of 76 +/- 10 min. An average PLT yield of 3.45 +/- 0.88 x 10(11) was collected in a product volume of 325 +/- 77 ml. The collection efficiency was 60.0 +/- 5.5%. WBC contamination of all units tested was 0.046 +/- 0.059 x 10(6) and the RBC content of the SDPs analysed at Day 0 was 0.014 +/- 0.003 x 10(9). The pH was well maintained over the storage period of 5 days. The data indicate that Fuji surge technique and continuous in-line leukocyte filtration allow for the collection of SDPs with high platelet yield and low leukocyte contamination, meeting the Council of Europe quality standards.

Improvement of plateletpheresis via technical modification on the MCS+

“…The study showed that 80·51% (95 in 118) of the products after modification was qualified (Table ). Previous study demonstrated that MCS+ was the highest capacity for leukodepletion compared with other two devices of Amicus and Accel (Bueno et al , ) because of a continuous‐filtration technique for leukodepletion (Seghatchian & Krailadsiri, ; Seghatchian et al , ; Moog, ). The modification on the MCS+ resulted in less than 1 × 10 8 L −1 leukocyte in the selected samples, which complied with the international standard requirements (MnU‐B, ).…”

A randomized crossover trial comparing three plateletpheresis machines

“…Some of the common features shared by these systems are the possibility of obtaining a SDP (3.5 × 10 11 platelets [PLTs] per unit in our center), with an integrated leukodepletion system, as well as a plasma unit. For leukodepletion, the MCS Plus uses a continuous‐filtration technique 3,4 . Accel uses fluidized particle‐bed technology, 5‐7 whereas Amicus uses the elutriation principle 5,8‐12 .…”

“…One differential feature is that the Amicus uses saline for set priming at the beginning of the procedure, whereas the MCS Plus and the Accel use acid citrate dextrose‐A (ACD‐A). Various publications 4,9 , 10,13 have explained the function of these systems in great detail. The manufacturing companies configured the machines at their discretion to obtain the best possible results according to the objectives of the study.…”

Multicomponent apheresis

“…All cytapheresis systems are capable to produce up to three white cell poor platelet units either in the apheresis procedure [9–12,14,15,25–34] or through an additional filtration step [27,29–31]. Multicomponent apheresis procedures using these techniques enable the concurrent collection of up to three platelet units alone or in combination with fresh frozen plasma fulfilling all quality criteria without the need of further manipulations [21,35–39].…”