Full-color structured illumination optical sectioning microscopy

Qian, Jia; Lei, Ming; Dan, Dan; Zhou, Xing; Yang, Yue; Yan, Shaohui; Min, Junwei; Yu, Xianghua

doi:10.1038/srep14513

Cited by 45 publications

(29 citation statements)

References 35 publications

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“…For the optical sectioning, we only used the intensity of each pixel of the raw images rather than the phase unwrapping method [ 35 , 36 , 37 ]. Sectioned images were obtained from the root-mean-square (RMS) of the sum of the squared differences between each raw image from the same focal plane.…”

Section: Resultsmentioning

confidence: 99%

Development of Three-Dimensional Dental Scanning Apparatus Using Structured Illumination

Ahn

Park

Kim

et al. 2017

Sensors

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We demonstrated a three-dimensional (3D) dental scanning apparatus based on structured illumination. A liquid lens was used for tuning focus and a piezomotor stage was used for the shift of structured light. A simple algorithm, which detects intensity modulation, was used to perform optical sectioning with structured illumination. We reconstructed a 3D point cloud, which represents the 3D coordinates of the digitized surface of a dental gypsum cast by piling up sectioned images. We performed 3D registration of an individual 3D point cloud, which includes alignment and merging the 3D point clouds to exhibit a 3D model of the dental cast.

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Section: Resultsmentioning

confidence: 99%

Development of Three-Dimensional Dental Scanning Apparatus Using Structured Illumination

Ahn

Park

Kim

et al. 2017

Sensors

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“…The acquisition of artifact‐free raw data is specifically important for most methods of secondary, calculation‐based image analyses, including superresolution microscopy with structured illumination (SIM), hyperspectral imaging, FRET or FRAP imaging, single particle tracking, and others. LED‐based SIM imaging has already been reported in the epifluorescence mode , and laser‐illuminated TIRF‐SIM is well established . Thus it is self‐evident that a combination of both is feasible.…”

Section: Discussionmentioning

confidence: 99%

Artifact‐free objective‐type multicolor total internal reflection fluorescence microscopy with light‐emitting diode light sources—Part I

Kögel

Urban

Schaefer

2019

Journal of Biophotonics

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Total internal reflection fluorescence excitation (TIRF) microscopy allows the selective observation of fluorescent molecules in immediate proximity to an interface between different refractive indices. Objective-type or prism-less TIRF excitation is typically achieved with laser light sources. We here propose a simple, yet optically advantageous light-emitting diode (LED)-based implementation of objective-type TIRF (LED-TIRF). The proposed LED-TIRF condenser is affordable and easy to set up at any epifluorescence microscope to perform multicolor TIRF and/or combined TIRF-epifluorescence imaging with even illumination of the entire field of view. Electrical control of LED light sources replaces mechanical shutters or optical modulators. LED-TIRF microscopy eliminates safety burdens that are associated with laser sources, offers favorable instrument lifetime and stability without active cooling. The non-coherent light source and the type of projection eliminate interference fringing and local scattering artifacts that are associated with conventional laser-TIRF. Unlike azimuthal spinning laser-TIRF, LED-TIRF does not require synchronization between beam rotation and the camera and can be monitored with either global or rolling shutter cameras. Typical implementations, such as live cell multicolor imaging in TIRF and epifluorescence of imaging of short-lived, localized translocation events of a Ca 2+ -sensitive protein kinase C α fusion protein are demonstrated. K E Y W O R D Slight-emitting diodes, multicolor cell imaging, objective-type prismless TIRF microscopy, protein kinase C translocation, total internal reflection fluorescence excitation

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“…Decreasing the depth of field requires a lower f-number that can be achieved, for instance, with a wider effective aperture (i.e., a wider lens). Equipping a camera drone with a lens several meters rather than millimeters wide is clearly infeasible, but would enable the kind of optical sectioning used in traditional light microscopes, which applies high numerical aperture optics [22][23][24].…”

Section: Methodsmentioning

confidence: 99%

Airborne Optical Sectioning

2018

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Drones are becoming increasingly popular for remote sensing of landscapes in archeology, cultural heritage, forestry, and other disciplines. They are more efficient than airplanes for capturing small areas, of up to several hundred square meters. LiDAR (light detection and ranging) and photogrammetry have been applied together with drones to achieve 3D reconstruction. With airborne optical sectioning (AOS), we present a radically different approach that is based on an old idea: synthetic aperture imaging. Rather than measuring, computing, and rendering 3D point clouds or triangulated 3D meshes, we apply image-based rendering for 3D visualization. In contrast to photogrammetry, AOS does not suffer from inaccurate correspondence matches and long processing times. It is cheaper than LiDAR, delivers surface color information, and has the potential to achieve high sampling resolutions. AOS samples the optical signal of wide synthetic apertures (30-100 m diameter) with unstructured video images recorded from a low-cost camera drone to support optical sectioning by image integration. The wide aperture signal results in a shallow depth of field and consequently in a strong blur of out-of-focus occluders, while images of points in focus remain clearly visible. Shifting focus computationally towards the ground allows optical slicing through dense occluder structures (such as leaves, tree branches, and coniferous trees), and discovery and inspection of concealed artifacts on the surface.

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Full-color structured illumination optical sectioning microscopy

Cited by 45 publications

References 35 publications

Development of Three-Dimensional Dental Scanning Apparatus Using Structured Illumination

Development of Three-Dimensional Dental Scanning Apparatus Using Structured Illumination

Artifact‐free objective‐type multicolor total internal reflection fluorescence microscopy with light‐emitting diode light sources—Part I

Airborne Optical Sectioning

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