Full-depth epidermis tomography using a Mirau-based full-field optical coherence tomography

Tsai, Chien-Chung; Chang, Chun-Lin; Hsu, Kuang–Yu; Ho, Tuan-Shu; Lin, Ming-Yi; Tjiu, Jeng-Wei; Huang, Sheng‐Lung

doi:10.1364/boe.5.003001

Cited by 48 publications

(44 citation statements)

References 35 publications

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“…Our preliminary study provided promising results on distinguishing precursor lesions from normal mucosa but future multicenter studies are necessary to validate the efficiency and accuracy of these criteria. Although demonstrated with μOCT, these understandings on the biological meaning of reflectance contrast should be directly applicable to other optical reflectance techniques including but not limited to RCM, OCM, and FF‐OCT . Looking forward, with the rapid advances in the development of cellular‐resolution optical reflectance technologies, diagnostic criteria based on these new understandings would complement the current morphology‐ or architecture‐based criteria to specifically decode the pathophysiological events within tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Although demonstrated with μOCT, these understandings on the biological meaning of reflectance contrast should be directly applicable to other optical reflectance techniques including but not limited to RCM, 13,33 OCM, 34 and FF-OCT. 52 Looking forward, with the rapid advances in the development of cellular-resolution optical reflectance technologies, 17,18,53,54 diagnostic criteria based on these new understandings would complement the current morphology-or architecture-based criteria to specifically decode the pathophysiological events within tissues. The success in this effort would ultimately have a substantial impact for the screening and surveillance of epithelial diseases by reducing costs and risks from unnecessary resections and sampling errors.…”

Section: Human Esophagus (Nonkeratinized) With Intraepithelial Neopmentioning

confidence: 99%

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Understanding optical reflectance contrast for real‐time characterization of epithelial precursor lesions

Chen

Liu

et al. 2019

Bioengineering & Transla Med

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Detecting early‐stage epithelial cancers and their precursor lesions are challenging as lesions could be subtle and focally or heterogeneously distributed over large mucosal areas. Optical coherence tomography (OCT) that enables wide‐field imaging of subsurface microstructures in vivo is a promising screening tool for epithelial diseases. However, its diagnostic capability has not been fully appreciated since the optical reflectance contrast is poorly understood. We investigated the back‐scattered intensities from clustered or packed nanometer scale intracellular scatterers using finite‐difference time‐domain method and 1‐μm resolution form of OCT, and uncovered that there existed correlations between the reflectance contrasts and the ultrastructural clustering or packing states of these scatterers, which allows us to interpret the physiological state of the cells. Specifically, both polarized goblet cells and foveolar cells exhibited asymmetric reflectance contrast, but they could be differentiated by the optical intensity of the mucin cup due to the different ultrastructural make‐ups of the mucin granules; keratinocytes could demonstrate varied cytoplasmic intensity and their cytoplasmic contrast was closely correlated with the packing state of keratin filaments. Further preliminary study demonstrated that these new understandings of OCT image contrast enables the characterization of precancerous lesions, which could complement the current morphology‐based criteria in realizing “virtual histology” and would have a profound impact for the screening and surveillance of epithelial cancers.

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Section: Discussionmentioning

confidence: 99%

Section: Human Esophagus (Nonkeratinized) With Intraepithelial Neopmentioning

confidence: 99%

Understanding optical reflectance contrast for real‐time characterization of epithelial precursor lesions

Chen

Liu

et al. 2019

Bioengineering & Transla Med

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“…A Mirau‐based FF‐OCM system was designed for in vivo imaging, having a rather long acquisition time of 230 ms, but yielding high quality images of human skin . Nevertheless, the use of a Mirau configuration has several drawbacks including a loss of light compared to the Linnik geometry due to the presence of the reference mirror in the illumination and detection paths, and the necessity to use 2 beam splitters (BSs).…”

Section: In Vivo Imaging Attempts With Ff‐ocmmentioning

confidence: 99%

A compact high‐speed full‐field optical coherence microscope for high‐resolution in vivo skin imaging

Ogien

Dubois

2018

Journal of Biophotonics

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A compact high-speed full-field optical coherence microscope has been developed for high-resolution in vivo imaging of biological tissues. The interferometer, in the Linnik configuration, has a size of 11 × 11 × 5 cm and a weight of 210 g. Full-field illumination with low-coherence light is achieved with a high-brightness broadband light-emitting diode. High-speed full-field detection is achieved by using part of the image sensor of a high-dynamic range CMOS camera. En face tomographic images are acquired at a rate of 50 Hz, with an integration time of 0.9 ms. The image spatial resolution is 0.9 μm × 1.2 μm (axial × transverse), over a field of view of 245 × 245 μm . Images of human skin, revealing in-depth cellular-level structures, were obtained in vivo and in real-time without the need for stabilization of the subject. The system can image larger fields, up to 1 × 1 mm , but at a reduced depth.

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“…Up to now, FF-OCT has been used in studies in developmental biology and cancer diagnosis. Examples include non-invasive 3D sub-cellular live imaging of preimplantation mouse embryos with no need of dye labeling for quantitatively measuring the factors that relate to early pattering and polarity in preimplantation embryonic [4] , obtaining en-face images of in vivo human lip [5] , and observing the blood vessel in dermis and tracing the flowing of the red blood cells [6] .…”

Section: Introductionmentioning

confidence: 99%

High-resolution imaging of biological tissue with full-field optical coherence tomography

Zhu

Gao

2015

SPIE Proceedings

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A new full-field optical coherence tomography system with high-resolution has been developed for imaging of cells and tissues. Compared with other FF-OCT (Full-field optical coherence tomography, FF-OCT) systems illuminated with optical fiber bundle, the improved Köhler illumination arrangement with a halogen lamp was used in the proposed FF-OCT system. High numerical aperture microscopic objectives were used for imaging and a piezoelectric ceramic transducer (PZT) was used for phase-shifting. En-face tomographic images can be obtained by applying the five-step phase-shifting algorithm to a series of interferometric images which are recorded by a smart camera. Three-dimensional images can be generated from these tomographic images. Imaging of the chip of Intel Pentium 4 processor demonstrated the ultrahigh resolution of the system (lateral resolution is 0.8 m μ ), which approaches the theoretical resolution 0.7En-face images of cells of onion show an excellent performance of the system in generating en-face images of biological tissues. Then, unstained pig stomach was imaged as a tissue and gastric pits could be easily recognized using FF-OCT system. Our study provides evidence for the potential ability of FFOCT in identifying gastric pits from pig stomach tissue. Finally, label-free and unstained ex vivo human liver tissues from both normal and tumor were imaged with this FFOCT system. The results show that the setup has the potential for medical diagnosis applications such liver cancer diagnosis.

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Full-depth epidermis tomography using a Mirau-based full-field optical coherence tomography

Cited by 48 publications

References 35 publications

Understanding optical reflectance contrast for real‐time characterization of epithelial precursor lesions

Understanding optical reflectance contrast for real‐time characterization of epithelial precursor lesions

A compact high‐speed full‐field optical coherence microscope for high‐resolution in vivo skin imaging

High-resolution imaging of biological tissue with full-field optical coherence tomography

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