Full field strain measurements of collagenous tissue by tracking fiber alignment through vector correlation

Quinn, Kyle P.; Winkelstein, Beth A.

doi:10.1016/j.jbiomech.2010.05.008

Cited by 24 publications

(25 citation statements)

References 12 publications

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“…To measure variability in fiber density surrounding the cell structure, the mean and standard deviation of fiber density from triangular elements within 20 mm of the cell structure edge were calculated. Similar to techniques used to smooth and display full-field strain maps, 25 the average fiber density at each point was computed from all elements defined using the point. The fiber density field was displayed with the fluorescence and SHG images.…”

Section: Analysis Of Collagen Organization By Shgmentioning

confidence: 99%

Hormonal Regulation of Epithelial Organization in a Three-Dimensional Breast Tissue Culture Model

Speroni

Whitt

Xylas

et al. 2014

Tissue Engineering Part C: Methods

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The establishment of hormone target breast cells in the 1970's resulted in suitable models for the study of hormone control of cell proliferation and gene expression using two-dimensional (2D) cultures. However, to study mammogenesis and breast tumor development in vitro, cells must be able to organize in three-dimensional (3D) structures like in the tissue. We now report the development of a hormone-sensitive 3D culture model for the study of mammogenesis and neoplastic development. Hormone-sensitive T47D breast cancer cells respond to estradiol in a dose-dependent manner by forming complex epithelial structures. Treatment with the synthetic progestagen promegestone, in the presence of estradiol, results in flat epithelial structures that display cytoplasmic projections, a phenomenon reported to precede side-branching. Additionally, as in the mammary gland, treatment with prolactin in the presence of estradiol induces budding structures. These changes in epithelial organization are accompanied by collagen remodeling. Collagen is the major acellular component of the breast stroma and an important player in tumor development and progression. Quantitative analysis of second harmonic generation of collagen fibers revealed that collagen density was more variable surrounding budding and irregularly shaped structures when compared to more regular structures; suggesting that fiber organization in the former is more anisotropic than in the latter. In sum, this new 3D model recapitulates morphogenetic events modulated by mammogenic hormones in the breast, and is suitable for the evaluation of therapeutic agents.

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Section: Analysis Of Collagen Organization By Shgmentioning

confidence: 99%

Hormonal Regulation of Epithelial Organization in a Three-Dimensional Breast Tissue Culture Model

Speroni

Whitt

Xylas

et al. 2014

Tissue Engineering Part C: Methods

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“…24 Briefly, a grid of virtual markers with 4-pixel spacing was assigned to the first alignment map created from the QPLI images. 24 The fiber alignment within a 9 9 9 pixel window surrounding each virtual marker was correlated with the fiber alignment in the subsequent frame. Marker displacements were calculated by maximizing the correlation of the local fiber alignment pattern between maps during the retraction.…”

Section: Analyses Of Joint Mechanics and Fiber Kinematics During Retrmentioning

confidence: 99%

“…Marker displacements were calculated by maximizing the correlation of the local fiber alignment pattern between maps during the retraction. 24 A mesh of three-node elements was generated to estimate strains through Delaunay triangulation using the virtual marker positions in the first alignment map. Using plane strain theory, Green's strain tensor was derived for each element in each alignment map.…”

Section: Analyses Of Joint Mechanics and Fiber Kinematics During Retrmentioning

confidence: 99%

“…A change in the vector correlation of a virtual marker between maps was used to determine the extent to which the fiber alignment surrounding that marker had changed after retraction. 24 Full-field maps of e 1 , c max , and the change in vector correlation after retraction were generated for each specimen. In those maps, a node was classified as sustaining either unrecovered strain or altered fiber alignment after retraction if the value at that node exceeded the measurement's entire distribution of error values obtained from all nodes in all specimens.…”

Section: Analyses Of Altered Joint Function and Microstructure Measuredmentioning

confidence: 99%

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Detection of Altered Collagen Fiber Alignment in the Cervical Facet Capsule After Whiplash-Like Joint Retraction

Quinn

Winkelstein

2011

Ann Biomed Eng

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The cervical facet joint has been identified as the source of pain in patients with whiplash-associated disorders, but most clinical studies report no radiographic evidence of tissue injury in these disorders. The goal of this study was to utilize quantitative polarized light imaging to assess the potential for altered collagen fiber alignment in human cadaveric cervical facet capsule specimens (n = 8) during and after a joint retraction simulating whiplash exposure. Although no evidence of ligament damage was detected during whiplash-like retraction, mechanical and microstructural changes were identified after loading. Retraction produced significant decreases in ligament stiffness (p = 0.0186) and increases in laxity (p = 0.0065). In addition, image analysis indicated that 21.1 ± 17.1% of the capsule sustained principal strains that were unrecovered immediately after retraction. Altered collagen fiber alignment was detected in 32.7 ± 22.9% of the capsule after retraction. The capsule regions with unrecovered strain and altered fiber alignment after retraction were significantly co-localized with each other (p < 0.0001), suggesting the altered mechanical function may relate to a change in the tissue's fiber organization. The identification of altered fiber alignment in this ligament following retraction without any tears implicates the whiplash kinematic as a potential cause of microstructural damage that is not detectable using standard clinical imaging techniques.

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“…49,76,79,98 Using this approach, collagen fiber realignment during tensile and posterior retraction loading of isolated human cadaveric facets, and tensile loading of isolated rat facets, has been defined. 70,72,[74][75][76] In particular, those studies collectively demonstrate that areas with the highest fiber realignment correspond to those regions with microstructural damage to the collagen matrix (FIGURE 3B). These studies have shown that areas of greatest fiber realignment are significantly correlated with subsequent rupture, 70,72,76 but do not necessarily correspond to areas of peak maximum principal or shear strain.…”

Section: Kinematics and Facet Tissue Injury During Whiplashmentioning

confidence: 72%

The Physiological Basis of Cervical Facet-Mediated Persistent Pain: Basic Science and Clinical Challenges

Ita¹,

Zhang²,

Holsgrove³

et al. 2017

Journal of Orthopaedic & Sports Physical Therapy

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FIGURE 1. Schematic illustrating the anatomy in the periphery of the facet joint and the relevant neuronal connections to the central nervous system. Afferents that innervate the facet joint and its capsular ligament have cell bodies in the DRG and synapse with neurons in the spinal dorsal horn. Nociceptive information is encoded by many types of afferents, including IB4-positive nonpeptidergic neurons and peptidergic fibers that produce neuropeptides, such as CGRP and substance P. Noxious stimuli are translated into electrical (eg, action potentials) and biochemical (eg, neurotransmitter) signals. In persistent pain, central sensitization occurs, with neuronal hyperexcitability and altered neurotransmitter production and release in the spinal dorsal horn. Abbreviations: CGRP, calcitonin gene-related peptide; DRG, dorsal root ganglion; IB4, isolectin B4.

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Full field strain measurements of collagenous tissue by tracking fiber alignment through vector correlation

Cited by 24 publications

References 12 publications

Hormonal Regulation of Epithelial Organization in a Three-Dimensional Breast Tissue Culture Model

Hormonal Regulation of Epithelial Organization in a Three-Dimensional Breast Tissue Culture Model

Detection of Altered Collagen Fiber Alignment in the Cervical Facet Capsule After Whiplash-Like Joint Retraction

The Physiological Basis of Cervical Facet-Mediated Persistent Pain: Basic Science and Clinical Challenges

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