2005
DOI: 10.1038/nature03353
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Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans

Abstract: A key challenge of functional genomics today is to generate well-annotated data sets that can be interpreted across different platforms and technologies. Large-scale functional genomics data often fail to connect to standard experimental approaches of gene characterization in individual laboratories. Furthermore, a lack of universal annotation standards for phenotypic data sets makes it difficult to compare different screening approaches. Here we address this problem in a screen designed to identify all genes … Show more

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Cited by 839 publications
(789 citation statements)
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References 21 publications
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“…Table 1). Based on the RNAi phenocopy Sonnichsen et al, 2005), cystatin is thought to be at least superficially dispensable for viability and gross morphology. However, the elevated level of transcripts for this gene in daf-2 adults provokes a question about the possible importance of this gene for longevity.…”
Section: Correlation Of the Sage Data With Differences In Longevitymentioning
confidence: 99%
“…Table 1). Based on the RNAi phenocopy Sonnichsen et al, 2005), cystatin is thought to be at least superficially dispensable for viability and gross morphology. However, the elevated level of transcripts for this gene in daf-2 adults provokes a question about the possible importance of this gene for longevity.…”
Section: Correlation Of the Sage Data With Differences In Longevitymentioning
confidence: 99%
“…Complete listing of all KEGG mappings including graphical representation is available at http:// www.nematode.net (Wylie et al, 2004). RNA interference (RNAi) technology was first developed and has been successfully applied to genome-wide gene silencing to reveal gene functions in C. elegans (Fire et al, 1998;Kamath et al, 2003;Rual et al, 2004;Sonnichsen et al, 2005). Caenorhabditis elegans RNAi phenotypes can be further extrapolated in understanding functions of orthologous genes in other nematodes, especially the parasitic nematodes in which complexity of obligate parasitic life cycles and lack of proper laboratory culture system makes high-throughput screening impractical .…”
Section: Functional Categorization and Other Sequence Analysismentioning
confidence: 99%
“…To know whether down-regulation of these proteins has a critical effect on larval development of C. elegans, we observed the RNAi phenotypes of these genes for two generations in CN condition. RNAi phenotypes of sams-1 (Gottschalk et al, 2005), pmt-2 (Sönnichsen et al, 2005), R07H5.8 (Sönnichsen et al, 2005), eef-1A.1 (Simmer et al, 2003), and rla-0 (Rual et al, 2004) were previously reported that they showed larval developmental defects. Unlike those previous reports, we found that RNAi of two out of six CS down-regulated genes, F25H2.10 (rla-0) and R07H5.8, caused severe larval arrest phenotype in the second generation in CN.…”
Section: Discussionmentioning
confidence: 91%