Full genome sequence of bovine alphaherpesvirus 2 (BoHV-2)

Pfaff, Florian; Neubauer-Juric, Antonie; Krebs, Stefan; Hauser, Andreas; Singer, Stefanie; Blum, Helmut; Hoffmann, Bernd

doi:10.1007/s00705-020-04895-x

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…Additionally, for V511 and V516, the parameters -nano-hq and -nano-raw were added, respectively. The Illumina reads were aligned to the contig representing LCHV to detect genome termini by visual inspection and read depth distribution [41]. Homopolymer regions in the genomes were inspected, and based on Illumina reads manually corrected, using Geneious Prime 2022.1.1 (Geneious version 2022.1 created by Biomatters, available from https://www.geneious.com; accessed on15 February 2024).…”

Section: Genome Assembly and Comparisonmentioning

confidence: 99%

Discovery, Pathogenesis, and Complete Genome Characterization of Lates calcarifer Herpesvirus

Simmelink,

Coolen,

Vogels

et al. 2024

Genes

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In 2015 and 2016, two Barramundi (Lates calcarifer) farms in Singapore reported a disease outbreak characterized by lethargic behavior, pronounced inappetence, generalized skin lesions, erosions of the fins and tail, and ultimately high mortality in their fish. Next-generation sequencing and PCR confirmed presence of a novel virus belonging to the Alloherpesviridae family, Lates calcarifer herpesvirus (LCHV), which was subsequently isolated and cultured. We characterize, for the first time, the complete genome of two cultured LCHV isolates. The genome contains a long unique region of approximately 105,000 bp flanked by terminal repeats of approximately 24,800 bp, of which the first 8.2 kb do not show any similarity to described genomes in the Alloherpesviridae family. The two cultured isolates share 89% nucleotide identity, and their closest relatives are the viruses belonging to the genus Ictalurivirus. Experimental infections using one of the cultured LCHV isolates resulted in identical clinical signs as originally described in the index farm, both in intraperitoneal-injection infected fish and cohabitant fish, with mortality in both groups. Histopathological analysis showed pronounced abnormalities in the gills. Virus culture and PCR analysis confirmed the replication of LCHV in the infected fish, and thus Koch’s postulates were fulfilled.

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Section: Genome Assembly and Comparisonmentioning

confidence: 99%

Discovery, Pathogenesis, and Complete Genome Characterization of Lates calcarifer Herpesvirus

Simmelink,

Coolen,

Vogels

et al. 2024

Genes

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“…The OriL is located between two E genes ( ul29 and ul30 ) that play essential roles in DNA replication while the 2 copies of OriS are surrounded by the IE genes icp4 and us1 in the internal repeat of the US region (IRS) and by icp4 and us12 genes within the terminal repeat of the US region (TRS). The Oris in other Simplexviruses such as HSV-2 27 and bovine alphaherpesvirus 2 (BoHV-2 28 ) are mapped in the same genomic loci.…”

Section: Introductionmentioning

confidence: 99%

Novel Herpesvirus Transcripts with Putative Regulatory Roles in DNA Replication and Global Transcription

Torma

Tombácz

Almsarrhad

et al. 2023

Preprint

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In the last couple of years, the rapid advances and decreasing costs of sequencing technologies have revolutionized transcriptomic research. Long-read sequencing (LRS) techniques are able to detect full-length RNA molecules in a single run without the need for additional assembly steps. LRS studies have revealed an unexpected transcriptomic complexity in a variety of organisms, including viruses. A number of transcripts with proven or putative regulatory role, mapping close to or overlapping the replication origins (Oris) and the nearby transcription activator genes, have been described in herpesviruses. In this study, we applied both newly generated and previously published LRS and short-read sequencing datasets to discover additional Ori-proximal transcripts in nine herpesviruses belonging to all of the three subfamilies (alpha, beta and gamma). We identified novel long non-coding RNAs (lncRNAs), as well as splice and length isoforms of mRNAs and lncRNAs. Furthermore, our analysis disclosed an intricate meshwork of transcriptional overlaps at the examined genomic regions. Our results suggest the existence of a super regulatory center, which controls both the replication and the global transcription through multilevel interactions between the molecular machineries.

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“…Its genome is typical of most simplexviruses, and it has been shown to have several genetic similarities to that of human alphaherpesvirus 1 (HHV1). In particular, the comparison of glycoprotein B (gB) sequences of BoAHV2 and HHV1 was used as a basis for the classification of their taxonomic group [8]. BoAHV2 is associated with two main clinical forms in cattle: bovine herpetic mammillitis (BHM) characterized by vesicular, ulcerated or necrotic lesions of the teat and udder skin, and pseudo-lumpy skin disease (PSLD) characterized by generalized superficial skin lesions [9].…”

Section: Introductionmentioning

confidence: 99%

Infectious Bovine Rhinotracheitis Post-Eradication Program in the Autonomous Province of Bolzano, Italy: A Retrospective Study on Potential Bovine Herpesvirus Type 2 Cross-Reactivity

Bettini,

Stella,

Precazzini

et al. 2023

Animals

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Bovine alphaherpesviruses, BoAHV, can cause respiratory, genital and neurological disorders. In particular, bovine alphaherpesvirus type 1 (BoAHV1) is one of the most significant ruminant pathogens worldwide and it can heavily damage the livestock industry. BoAHV1 can cause infectious bovine rhinotracheitis (IBR) along with fertility disorders. Bovine alphaherpesvirus type 2 (BoAHV2) can cause two different conditions as well: pseudo-lumpy skin disease (PSLD) and bovine herpetic mammillitis (BHM). The autonomous province of Bolzano (Italy) has adopted several strategies to control and eradicate IBR, and it was declared in 2000 to be IBR-free by the European Commission. Since 2001, a post-eradication monitoring program has overseen the serological analysis of bulk milk and, in the presence of a positive result, a follow-up examination is performed on the individual blood serum of all bovines older than 24 months that belong to bulk milk-positive herds. Despite the detection of positives in both bulk milk and serum samples, South Tyrol has been declared IBR-free, as these positives have never been confirmed through seroneutralization. Between 2014 and 2022, approximately 41,000 bulk milk (averaging 4300 samples/year) and 3229 serum samples were tested for BoAHV1. The aim of this study was to evaluate the post-eradication program for IBR with a particular focus on the potential cross-reactivity with BoAHV2; for this reason, serum samples were also tested for BoAHV2 antibodies. This study could be of great importance for those countries that submit herds to an IBR monitoring and eradication program; performing further analyses to confirm and explain false positive outcomes would increase the reliability of the obtained results.

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Full genome sequence of bovine alphaherpesvirus 2 (BoHV-2)

Cited by 3 publications

References 17 publications

Discovery, Pathogenesis, and Complete Genome Characterization of Lates calcarifer Herpesvirus

Discovery, Pathogenesis, and Complete Genome Characterization of Lates calcarifer Herpesvirus

Novel Herpesvirus Transcripts with Putative Regulatory Roles in DNA Replication and Global Transcription

Infectious Bovine Rhinotracheitis Post-Eradication Program in the Autonomous Province of Bolzano, Italy: A Retrospective Study on Potential Bovine Herpesvirus Type 2 Cross-Reactivity

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