Full Genome Sequence of Egg Drop Syndrome Virus Strain FJ12025 Isolated from Muscovy Duckling

Fu, Guanghua; Chen, Hongmei; Huang, Yu; Cheng, Longfei; Fu, Qiuling; Shi, Shaohua; Wan, Chunhe; Chen, Cuiteng; Lin, Juan

doi:10.1128/genomea.00623-13

Cited by 9 publications

(9 citation statements)

References 5 publications

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“…The Atadenovirus genus was observed to contain overall lower genomic G+C percentage in the avian and ruminant viruses that were initially sequenced [ 2 , 9 , 23 ], with an exception for PsAdV-3, which was shown to contain a balanced G+C content (53.54%) [ 11 ]. Similar to the balanced G+C contents in reptilian atadenoviruses and PsAdV-3 genome sequence [ 5 , 10 , 11 ], the novel PaAdV-1 genome sequence also showed a balanced G+C content (53.70), in contrast to the G+C content seen with most of the of avian and ruminant atadenoviruses ( Table 2 ).…”

Section: Discussionmentioning

confidence: 99%

“…Because of the limited sequence information for these passerine adenoviruses, there is still considerable uncertainty about their phylogenetic relationship to each other and other atadenoviruses. Additionally, only two avian atadenoviruses, the psittacine adenovirus 3 (PsAdV-3) and duck atadenovirus (DAdV), have been fully sequenced to date [ 11 , 23 , 24 ]. In this paper, we present the complete genome sequence of an atadenovirus from a passerine bird.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Characterisation of a Novel and Highly Divergent Passerine Adenovirus 1

Athukorala

Forwood

Phalen

et al. 2020

Viruses

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Wild birds harbour a large number of adenoviruses that remain uncharacterised with respect to their genomic organisation, diversity, and evolution within complex ecosystems. Here, we present the first complete genome sequence of an atadenovirus from a passerine bird that is tentatively named Passerine adenovirus 1 (PaAdV-1). The PaAdV-1 genome is 39,664 bp in length, which was the longest atadenovirus to be sequenced, to the best of our knowledge, and contained 42 putative genes. Its genome organisation was characteristic of the members of genus Atadenovirus; however, the novel PaAdV-1 genome was highly divergent and showed the highest sequence similarity with psittacine adenovirus-3 (55.58%). Importantly, PaAdV-1 complete genome was deemed to contain 17 predicted novel genes that were not present in any other adenoviruses sequenced to date, with several of these predicted novel genes encoding proteins that harbour transmembrane helices. Subsequent analysis of the novel PaAdV-1 genome positioned phylogenetically to a distinct sub-clade with all others sequenced atadenoviruses and did not show any obvious close evolutionary relationship. This study concluded that the PaAdV-1 complete genome described here is not closely related to any other adenovirus isolated from avian or other natural host species and that it should be considered a separate species.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Characterisation of a Novel and Highly Divergent Passerine Adenovirus 1

Athukorala

Forwood

Phalen

et al. 2020

Viruses

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“…EDS is characterized by healthy laying hens apparently changing to produce eggs of poor quality, with clinical signs including soft, thin-shelled eggs, and shell-less eggs ( Huang J. et al, 2015 ). EDS was first reported in Holland in 1976, and the infection then spread throughout the world and more recently appeared in China ( Baxendale, 1978 ; McFerran and Smyth, 2000 ; Ezeibe et al, 2008 ; Fu et al, 2013 ). The causative agent, egg drop syndrome virus (EDSV) belong to the genus Atadenovirus within the family Adenoviridae, which are non-enveloped viruses with an approximately 30–35 kb linear double-stranded DNA genome ( Fu et al, 2013 ; Larson et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

“…EDS was first reported in Holland in 1976, and the infection then spread throughout the world and more recently appeared in China ( Baxendale, 1978 ; McFerran and Smyth, 2000 ; Ezeibe et al, 2008 ; Fu et al, 2013 ). The causative agent, egg drop syndrome virus (EDSV) belong to the genus Atadenovirus within the family Adenoviridae, which are non-enveloped viruses with an approximately 30–35 kb linear double-stranded DNA genome ( Fu et al, 2013 ; Larson et al, 2015 ). The adenovirus size range is 75–80 nm, and the replicative cycle is divided into two phases: early protein expression (E1–E4) and late protein expression (L1–L5) ( Larson et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Autophagy Benefits the Replication of Egg Drop Syndrome Virus in Duck Embryo Fibroblasts

Wang

Yang

et al. 2018

Front. Microbiol.

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Egg drop syndrome virus (EDSV) is an economically important pathogen with a broad host range, and it causes disease that leads to markedly decreased egg production. Although EDSV is known to induce apoptosis in duck embryo fibroblasts (DEFs), the interaction between EDSV and its host needs to be further researched. Here, we provide the first evidence that EDSV infection triggers autophagy in DEFs through increases in autophagosome-like double-membrane vesicles, the conversion of LC3-I to LC3-II, and LC3 colocalization with viral hexon proteins. Conversely, P62/SQSTM1 degradation, LC3-II turnover, and colocalization of LAMP and LC3 confirmed that EDSV infection triggers complete autophagy. Furthermore, we demonstrated that inhibition of autophagy by chloroquine (CQ) and 3-methyladenine (3MA) or RNA interference targeting ATG-7 decreased the yield of EDSV progeny. In contrast, induction of autophagy by rapamycin increased the EDSV progeny yield. In addition, we preliminarily demonstrated that the class I phosphoinositide 3-kinase (PI3K)/Akt/mTOR pathway contributes to autophagic induction following EDSV infection. Altogether, these finding lead us to conclude that EDSV infection induces autophagy, which benefits its own replication in host cells. These findings provide novel insights into EDSV–host interactions.

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“…International Committee on Taxonomy of Viruses), with viruses that infect a diverse range of animals, including avian, ruminant, reptile and marsupial hosts [ 9, 17–20 ]. A complete or partial genome sequence has been determined for the genus members, including ovine adenovirus 7 (species Ovine atadenovirus D , OAdV-D; type species of the genus) [ 16 ], bovine adenovirus 4 [ 8 ] (within species Bovine atadenovirus D , BAdV-D), bovine adenovirus 6, duck adenovirus 1 [ 21 ], lizard adenovirus 2 [ 9 ], possum adenovirus 1 [ 17 ], psittacine adenovirus 3 [ 19 ] and snake adenovirus 1 [ 12, 14 ].…”

Section: Introductionmentioning

confidence: 99%

Whole-genome sequences of Odocoileus hemionus deer adenovirus isolates from deer, moose and elk are highly conserved and support a new species in the genus Atadenovirus

Miller

Cornish

Creekmore

et al. 2017

Journal of General Virology

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We present the first complete genome sequence of Odocoileus hemionus deer adenovirus 1 (OdAdV-1). This virus can cause sporadic haemorrhagic disease in cervids, although epizootics with high mortality have occurred in California. OdAdV-1 has been placed in the genus Atadenovirus, based on partial hexon, pVIII and fibre genes. Ten field isolates recovered from naturally infected mule deer (Odocoileus hemionus), white-tailed deer (Odocoileus virginiana) and moose (Alces alces) from Wyoming, black-tailed deer (Odocoileus hemionus columbianus) from California, and Rocky Mountain elk (Cervus elaphus nelsoni) from Colorado and Washington state were sequenced. The genome lengths ranged from 30 620 to 30 699 bp, contained the predicted proteins and gene organization typical of members of genus Atadenovirus, and had a high percentage of A/T nucleotides (66.7 %). Phylogenic analysis found that the closest ancestry was with ruminant atadenoviruses, while a divergence of the hexon, polymerase and penton base proteins of more than 15 % supports classification as a new species. Genetic global comparison between the 10 isolates found an overall 99 % identity, but greater divergence was found between those recovered from moose and elk as compared to deer, and a single variable region contained most of these differences. Our findings demonstrate that OdAdV-1 is highly conserved between 10 isolates recovered from multiple related cervid species, but genotypic differences, largely localized to a variable region, define two strains. We propose that the virus type name be changed to cervid adenovirus 1, with the species name Cervid atadenovirus A. Sequence data were used to develop molecular assays for improved detection and genotyping.

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Full Genome Sequence of Egg Drop Syndrome Virus Strain FJ12025 Isolated from Muscovy Duckling

Cited by 9 publications

References 5 publications

Molecular Characterisation of a Novel and Highly Divergent Passerine Adenovirus 1

Molecular Characterisation of a Novel and Highly Divergent Passerine Adenovirus 1

Autophagy Benefits the Replication of Egg Drop Syndrome Virus in Duck Embryo Fibroblasts

Whole-genome sequences of Odocoileus hemionus deer adenovirus isolates from deer, moose and elk are highly conserved and support a new species in the genus Atadenovirus

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