Full genome sequences and preliminary molecular characterization of three tick-borne encephalitis virus strains isolated from ticks and a bank vole in Slovak Republic

Frey, Stefan; Eßbauer, Sandra; Zöller, Gudrun; Klempa, Boris; Dobler, Gerhard; Pfeffer, Martin

doi:10.1007/s11262-013-0985-0

Cited by 14 publications

(7 citation statements)

References 22 publications

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“…Modern molecular techniques enabled further characterisation of virus strains formerly isolated from ticks and rodents in Slovakia, comparison of nucleotide sequences of strains isolated from vectors and hosts, and contributed to the understanding of local genetic diversity and history of evolution and spread of TBEV strains in Central Europe (Weidmann et al 2013;Frey et al 2014). Similarity of the isolates from I. ricinus and bank vole with the Neudoerfl strain and high degree of identity of the stains from the vector and bank vole were revealed, suggesting no specific association of TBEV strains to hosts (Frey et al 2014). Moreover, phylogenetic analyses indicated a recent spread of virus strains in Central Europe from east to the west, particularly from the Czech Republic and Slovakia to Germany via Danube River system (Weidmann et al 2013).…”

Section: Tick-borne Encephalitis Virusmentioning

confidence: 99%

Ticks and their epidemiological role in Slovakia: from the past till present

et al. 2021

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In Slovakia, 22 tick species have been found to occur to date. Among them, Ixodes ricinus, Dermacentor reticulatus, D. marginatus and marginally Haemaphysalis concinna, H. inermis and H. punctata have been identified as the species of public health relevance. Ticks in Slovakia were found to harbour and transmit zoonotic and/or potentially zoonotic agents such as tickborne encephalitis virus (TBEV), spirochaetes of the Borrelia burgdorferi sensu lato (s.l.) complex, the relapsing fever sprirochaete Borrelia miyamotoi, bacteria belonging to the orders Rickettsiales (Rickettsia spp., Anaplasma phagocytophilum, Neoehrlichia mikurensis), Legionellales (Coxiella burnetii), and Thiotrichales (Francisella tularensis), and Babesia spp. parasites (order Piroplasmida). Ixodes ricinus is the principal vector of the largest variety of microorganisms including viruses, bacteria and piroplasms. TBEV, B. burgdorferi s.l., rickettsiae of the spotted fever group, C. burnetii and F. tularensis have been found to cause serious diseases in humans, whereas B. miyamotoi, A. phagocytophilum, N. mikurensis, Babesia microti, and B. venatorum pose lower or potential risk to humans. Distribution of TBEV has a focal character. During the last few decades, new tick-borne encephalitis (TBE) foci and their spread to new areas have been registered and TBE incidence rates have increased. Moreover, Slovakia reports the highest rates of alimentary TBE infections among the European countries. Lyme borreliosis (LB) spirochaetes are spread throughout the distribution range of I. ricinus. Incidence rates of LB have shown a slightly increasing trend since 2010. Only a few sporadic cases of human rickettsiosis, anaplasmosis and babesiosis have been confirmed thus far in Slovakia. The latest large outbreaks of Q fever and tularaemia were recorded in 1993 and 1967, respectively. Since then, a few human cases of Q fever have been reported almost each year. Changes in the epidemiological characteristics and clinical forms of tularaemia have been observed during the last few decades. Global changes and development of modern molecular tools led to the discovery and identification of emerging or new tick-borne microorganisms and symbionts with unknown zoonotic potential. In this review, we provide a historical overview of research on ticks and tick-borne pathogens in Slovakia with the most important milestones and recent findings, and outline future directions in the investigation of ticks as ectoparasites and vectors of zoonotic agents and in the study of tick-borne diseases.Keywords Tick-borne pathogens . Tick-borne encephalitis virus . Borrelia . Anaplasma . Rickettsia . Coxiella burnetii . Francisella tularensis . Babesia

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Section: Tick-borne Encephalitis Virusmentioning

confidence: 99%

Ticks and their epidemiological role in Slovakia: from the past till present

et al. 2021

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“…3). The closest genetic relatives (bootstrap support = 90 %) are a cluster of sequences from central European strains from Wagnitz, Austria; Bavaria, Germany, Joutseno; Finland, central Bohemia and Czech Republic and several strains from Slovakia (Formanová et al, 2015;Frey et al, 2014Frey et al, , 2013Kupča et al, 2010;Uzcategui et al, 2012). Phylogenetic analyses of 56 TBEV-Eu E genes are available in supplementary material S1.…”

Section: Phylogenetic Analysismentioning

confidence: 99%

Phylogenetic characterization of tick-borne encephalitis virus from Bornholm, Denmark

Andersen

Bestehorn

Chiţimia-Dobler

et al. 2019

Ticks and Tick-borne Diseases

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“…Of late, D. reticulatus has extended its former geographical distribution by at least 200 km further to the north and by approximately 300 m into higher altitudes up to 520 m above sea D. reticulatus tick is associated with a number of different pathogens and currently considered the second most significant reservoir and vector of numerous pathogens causing bacterial, protozoal, rickettsial, and viral diseases in its hosts [38,43,[64][65][66][67][68]. Its role in the transmission of disease to humans is currently small; however, it might play an important role in the maintenance of pathogens in enzootic cycles [69][70][71][72].…”

Section: Findings Of the Mhv-68 In Ticksmentioning

confidence: 99%

Murine Gammaherpesvirus 68 (MHV-68), a Newly Discovered Tick Borne Virus

Kúdelová¹,

Štibrániová²

2019

Ticks and Tick-Borne Pathogens

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MHV-68, closely related to human gammaherpesviruses (Epstein-Barr virus and Kaposi's sarcoma herpesvirus), is a natural pathogen of murid rodents commonly infested with ticks. After the first finding of MHV-68 in immature Ixodes ricinus ticks removed from wild green lizards, its occurrence was proved in free-living Dermacentor reticulatus, I. ricinus, and Haemaphysalis concinna ticks. Next, finding of live MHV-68 in salivary glands, intestine, and ovaries of D. reticulatus ticks strongly supported the idea that MHV-68 could be transmitted from infected to uninfected host via blood-feeding ticks. Recently, experimental transmission of MHV-68 between I. ricinus ticks and mouse and vice versa proved that MHV-68 could be vertically and horizontally transmitted from F0 to F1 tick generation, and thus, MHV-68 is a tick-borne virus (arbovirus). Therefore, ticks commonly attack humans transmitting important pathogens (e.g., tick-borne encephalitis virus and the Lyme disease spirochete); there is the speculation that MHV-68 can also infect humans via ticks. Earlier studies documented antibodies to MHV-68 in the sera of laboratory workers, hunters, and general population as well. In future, we need to carefully test whether people bitten by ticks are at real risk of infection with MHV-68 that normally infects murid rodents, and what effect it may have.

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Full genome sequences and preliminary molecular characterization of three tick-borne encephalitis virus strains isolated from ticks and a bank vole in Slovak Republic

Cited by 14 publications

References 22 publications

Ticks and their epidemiological role in Slovakia: from the past till present

Ticks and their epidemiological role in Slovakia: from the past till present

Phylogenetic characterization of tick-borne encephalitis virus from Bornholm, Denmark

Murine Gammaherpesvirus 68 (MHV-68), a Newly Discovered Tick Borne Virus

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