Full-length but not truncated CD34 inhibits hematopoietic cell differentiation of M1 cells

Fackler, MJ; Krause, DS; Smith, OM; Civin, CI; May, Win

doi:10.1182/blood.v85.11.3040.bloodjournal85113040

Cited by 91 publications

(44 citation statements)

References 39 publications

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“…Poblet et al (2006) showed that the function of CD34 may be related to the adhesion of the external root sheath to the stroma in anagen follicles. In addition, several other studies on the function of CD34 also indicated that CD34 can serve as an adhesion molecule to promote cell adhesion (Fackler et al 1995;Knapp et al 1995;Gangenahalli et al 2006). In our study, the expression of CD34 in Yorkshire pigs was barely detectable at the uterine luminal epithelium on day 15 of pregnancy and increased dramatically in all regions of uterine luminal epithelium on day 26 of pregnancy.…”

Section: Discussionsupporting

confidence: 68%

Expression Pattern of CD34 at the Maternal–Foetal Interface During Pregnancy in Pigs

Hong

Hou

et al. 2013

Reprod Domestic Animals

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The pig exhibits a non-invasive, epitheliochorial placentation. Adhesion molecules are indispensable for successful implantation and establishment of placentation. CD34 is an adhesion molecule belonging to the immunoglobulin superfamily (IgSF). To take the first step to investigate the role of CD34 in placentation, we examined the expression pattern of CD34 at the maternal-foetal interface in Yorkshire gilts on days 15, 26, 50 or 95 and in Meishan gilts on days 26, 50 or 95 of pregnancy (n = 3 gilts/breed/day of pregnancy) by immunohistochemical technique. The CD34-positive signals were detected in uterine luminal epithelium and trophectoderm in Yorkshire pigs; the staining for CD34 was located in trophectoderm but barely detectable at the uterine luminal epithelium on day 15 of pregnancy. Then, the expression of CD34 increased dramatically in both the uterine luminal epithelium and trophectoderm by day 26, and weak staining intensity was observed at the maternal-foetal interface on days 50 and 95 of pregnancy. The expression pattern of CD34 in Meishan pigs is similar to that in Yorkshire pigs except that only a few positive signals were observed at the luminal epithelium on day 26 of pregnancy. These results suggest that CD34 may be involved in mediating the cell-to-cell adhesion between trophectoderm and the luminal epithelial cells during early pregnancy in pigs.

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Section: Discussionsupporting

confidence: 68%

Expression Pattern of CD34 at the Maternal–Foetal Interface During Pregnancy in Pigs

Hong

Hou

et al. 2013

Reprod Domestic Animals

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“…AAF was administered by gavage (2.0 mg/d) for 8 days, and a standard two-thirds PH was performed after 4 days of AAF etic cell differentiation. 2 Results from other studies have also treatment. Animals were killed at various intervals (between 0 and suggested the possible involvement of CD34 in leukocyte salt solution followed by 0.1% collagenase and 0.1% pronase in Blots were washed twice each with 11 SSC/0.1% sodium dodecyl buffered William's solution.…”

Section: Methodsmentioning

confidence: 97%

Partial cloning of rat CD34 cDNA and expression during stem cell- dependent liver regeneration in the adult rat

Omori¹

1997

Hepatology

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adhesion and ''homing'' during the inflammatory process, The sialomucin CD34 is expressed on human and mouse and in stem/progenitor cell localization/adhesion in the bone hematopoietic stem cells and is used as an important marker marrow. 1 CD34 is not only expressed in hematopoietic stem/ for isolating the hematopoietic stem/progenitor cells. The progenitor cells, but also in small-vessel endothelial cells, involvement of hepatic stem cells in liver regeneration under and embryonic fibroblast. 1 Human, 3,4 mouse, 5-7 and canine 8 certain conditions in adult rats is now well supported. The homologues of CD34 have been reported, and extensive studobjective of the present research was to explore the idea that ies have shown a high degree of homology at the amino acid CD34 might also be expressed on hepatic stem cell progeny.level, especially in the intracellular domain (ú90%) among Polymerase chain reaction (PCR)-based cloning of rat CD34 these species, but not in the extracellular domain (Ç45%), was partially accomplished. During the hepatic stem cell actisuggesting a functional importance of the intracellular part. vation (2-acetylaminofluorene/partial hepatectomy [AAF/PH]In light of these observations, we decided to examine the model), the CD34 transcripts were increased and reached the expression of CD34 in the rat and to test the possible involvepeak level between 9 and 12 days after partial hepatectomy ment of this gene and its product in the activation and expanwhen the progenitor cells (e.g., oval cells, early hepatocytes in sion of hepatic stem cells 9 (and references therein) during basophilic foci, and intestinal type of cells) are most abundant.liver regeneration. Both in situ hybridization and immunohistochemistry, usingIt is well known that mature hepatocytes proliferate after anti-mouse CD34 antibody, which recognizes the cytoplasmic partial hepatectomy (PH), resulting in regeneration of the domain, clearly showed the expression of CD34 on oval cells liver mass. However, if PH is performed when the hepatoas well as on endothelial cells of large hepatic vessels. In cytes are mitoinhibited and/or functionally compromised, the addition, bile ductular epithelial (BDE) cells both in the AAF/ bile duct-like cells, commonly referred to as oval cells, ex-PH model and in normal liver expressed CD34, suggesting a pand instead of mature hepatocytes and regenerate the liver. close relationship between BDE cells and the hepatic stem-The oval cells are thought to be the progeny of the normally cell compartment. Taken together, the data indicate that dormant hepatic stem-cell compartment. 9 We have pre-CD34 would, similar to its role in the hematopoietic system, viously shown by using an experimental model that combe an important probe for characterizing the cellular biology bines PH and the administration of a noncarcinogenic dose of the hepatic stem-cell compartment. (HEPATOLOGY 1997; of 2-acetylaminofluorene (AAF/PH model) 10 that oval cells 26:720-727. ) are capable of differentiating into hepatocytes. In th...

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“…The CD34 ' progenitor cell line, M1, (light blue) can be induced to downregulate CD34 and differentiate into macrophages (dark blue) upon the addition of IL-6 or LIF. Forced expression of CD34 in this cell line appears to block differentiation [18]. (C) High endothelial venule (HEV)-specific epitopes present on CD34 facilitate L-selectin-mediated adhesion of naïve lymphocytes in lymph nodes [3].…”

Section: Mast Cells As a Model To Study Cd34 Functionmentioning

confidence: 99%

“…In another approach to elucidating the function of CD34, it was expressed in the myeloblastic cell line, M1 [18], ( Figure 1B). This line can normally be induced to differentiate into macrophages, but CD34 appears to block this differentiation pathway.…”

mentioning

confidence: 99%

CD34 is a Key Regulator of Hematopoietic Stem Cell Trafficking to Bone Marrow and Mast Cell Progenitor Trafficking in the Periphery

Nielsen

McNagny

2009

Microcirculation

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CD34 is a cell-surface sialomucin widely used for hematopoietic stem cell purification and as a marker of most vascular endothelial cells, including those of capillaries in the majority of tissues. Surprisingly, despite extensive research, the function of this sialomucin has remained elusive, with proposed roles ranging from enhancing proliferation or inhibiting differentiation to acting as a proadhesive L-selectin ligand. Here, we review our recent studies, which suggest that CD34 does, indeed, play a role in leukocyte and HSC trafficking, but that this is through its action as a regulated blocker of cell adhesion and enhancer of migration.

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Full-length but not truncated CD34 inhibits hematopoietic cell differentiation of M1 cells

Cited by 91 publications

References 39 publications

Expression Pattern of CD34 at the Maternal–Foetal Interface During Pregnancy in Pigs

Expression Pattern of CD34 at the Maternal–Foetal Interface During Pregnancy in Pigs

Partial cloning of rat CD34 cDNA and expression during stem cell- dependent liver regeneration in the adult rat

CD34 is a Key Regulator of Hematopoietic Stem Cell Trafficking to Bone Marrow and Mast Cell Progenitor Trafficking in the Periphery

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