Full-Length Complementary DNA and the Derived Amino Acid Sequence of Horse Uteroglobin1

Müller-Schöttle, Frank; Bogusz, Agata M.; Grötzinger, Joachim; Herrler, Andreas; Krusche, Claudia A.; Beier-Hellwig, K.; Beier, Henning M.

doi:10.1095/biolreprod66.6.1723

Cited by 17 publications

(14 citation statements)

References 32 publications

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“…Tissues were selected on the basis of their strong total SCGB1A1 transcript expression [11]. Primers were developed in conserved regions of the genes to avoid gene-specific preference during the amplification process.…”

Section: Resultsmentioning

confidence: 99%

“…Expression of SCGB1A1 has also variously been described in extra-pulmonary tissues from diverse species. In horses, SCGB1A1 transcripts were present in uterine and prostatic tissues and absent in liver, kidney, heart, spleen, thyroid, pituitary and adrenal gland tissues [11]. …”

Section: Introductionmentioning

confidence: 99%

“…This organizational structure is remarkably conserved between species; however, the length of the SCGB1A1 genomic locus fluctuates [12-17]. In horses, the first reported sequence was described as a unique cDNA and was ascribed to a single gene [11]. However, the recent availability of the complete Equus caballus genome sequence provided evidence of three highly similar SCGB1A1 gene sequences on chromosome 12, suggesting the horse has diverged from the “single copy” SCGB1A1 consensus.…”

Section: Introductionmentioning

confidence: 99%

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Multiple secretoglobin 1A1 genes are differentially expressed in horses

et al. 2012

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BackgroundSecretoglobin 1A1 (SCGB 1A1), also called Clara cell secretory protein, is the most abundantly secreted protein of the airway. The SCGB1A1 gene has been characterized in mammals as a single copy in the genome. However, analysis of the equine genome suggested that horses might have multiple SCGB1A1 gene copies. Non-ciliated lung epithelial cells produce SCGB 1A1 during inhalation of noxious substances to counter airway inflammation. Airway fluid and lung tissue of horses with recurrent airway obstruction (RAO), a chronic inflammatory lung disease affecting mature horses similar to environmentally induced asthma of humans, have reduced total SCGB 1A1 concentration. Herein, we investigated whether horses have distinct expressed SCGB1A1 genes; whether the transcripts are differentially expressed in tissues and in inflammatory lung disease; and whether there is cell specific protein expression in tissues.ResultsWe identified three SCGB1A1 gene copies on equine chromosome 12, contained within a 512-kilobase region. Bioinformatic analysis showed that SCGB1A1 genes differ from each other by 8 to 10 nucleotides, and that they code for different proteins. Transcripts were detected for SCGB1A1 and SCGB1A1A, but not for SCGB1A1P. The SCGB1A1P gene had most inter-individual variability and contained a non-sense mutation in many animals, suggesting that SCGB1A1P has evolved into a pseudogene. Analysis of SCGB1A1 and SCGB1A1A sequences by endpoint-limiting dilution PCR identified a consistent difference affecting 3 bp within exon 2, which served as a gene-specific “signature”. Assessment of gene- and organ-specific expression by semiquantitative RT-PCR of 33 tissues showed strong expression of SCGB1A1 and SCGB1A1A in lung, uterus, Fallopian tube and mammary gland, which correlated with detection of SCGB 1A1 protein by immunohistochemistry. Significantly altered expression of the ratio of SCGB1A1A to SCGB1A1 was detected in RAO-affected animals compared to controls, suggesting different roles for SCGB 1A1 and SCGB 1A1A in this inflammatory condition.ConclusionsThis is the first report of three SCGB1A1 genes in a mammal. The two expressed genes code for proteins predicted to differ in function. Alterations in the gene expression ratio in RAO suggest cell and tissue specific regulation and functions. These findings may be important for understanding of lung and reproductive conditions.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multiple secretoglobin 1A1 genes are differentially expressed in horses

et al. 2012

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“…During the past two decades, the isolation and characterization of cDNAs encoding UG from the mouse (14), pig (15), Syrian hamster (16), horse (17), and human (18,19) have been reported.…”

Section: Ifn-␥ Stimulates the Expression Of A Novel Secretoglobin Thatmentioning

confidence: 99%

IFN-γ Stimulates the Expression of a Novel Secretoglobin That Regulates Chemotactic Cell Migration and Invasion

Choi

Ray

Zhang

et al. 2004

The Journal of Immunology

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IFNs are a family of cytokines that alert the immune system against viral infections of host cells. The IFNs (IFN-α, IFN-β, and IFN-γ) interact with specific cellular receptors and stimulate the production of second messengers, leading to the expression of antiviral and immunomodulatory proteins. We report in this study that IFN-γ stimulates the expression of a novel gene that encodes a protein with 30% amino acid sequence identity with uteroglobin, the founding member of the newly formed Secretoglobin (SCGB) superfamily. We named this protein IFN-γ-inducible SCGB (IIS), because its expression in lymphoblast cells is augmented by IFN-γ treatment. IIS is expressed in virtually all tissues, and the highest level of expression is detectable in lymph nodes, tonsil, cultured lymphoblasts, and the ovary. Interestingly, although the expression of IIS mRNA is not significantly different in resting lymphoid cells, it is markedly elevated in activated CD8+ and CD19+ cells. Furthermore, treatment of lymphoblast cells with IIS antisense phosphorothioate (S)-oligonucleotides prevents chemotactic migration and invasion. Taken together, these results raise the possibility that this novel SCGB has immunological functions.

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“…This globular protein has been suggested to act as a carrier, in particular for lipophilic molecules such as progesterone, phospholipids, and polychlorinated biphenyls. 362 Furthermore, uteroglobin has been shown to be able to modulate immunological activity. 363 Uteroglobin may be particularly suitable as a marker molecule because it is one of the few markers whose intrauterine concentration decreases when the implantation window closes.…”

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confidence: 99%

Endometrial receptivity: Clinical assessment in relation to fertility, infertility, and antifertility

Makker

Singh

2006

Med. Res. Rev.

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Fertility in humans and other mammalian species depends absolutely on synchronous events that render the developing blastocyst and the receiving uterus competent for implantation. Endometrial receptivity is defined as the period during which the endometrial epithelium acquires functional, but transient, ovarian steroid-dependent status supportive to blastocyst acceptance and implantation. Once inside the uterus, the blastocyst is surrounded by an intact luminal epithelium, which is considered to act as barrier to its attachment, except for this short period of high endometrial receptivity to blastocyst signal(s). Its transport and permeability properties, in conjunction with cellular action of the endometrium and the embryo, have been suggested to influence creation and maintenance of informational and nutritional status of uterine luminal milieu. This period, also termed as the 'window of implantation,' is limited to days 20-24 of menstrual cycle in humans. However, establishment of endometrial receptivity is still a biological mystery that remains unsolved despite marked advances in our understanding of endometrial physiology following extensive research associated with its development and function. This review deals with various structural, biochemical, and molecular events in the endometrium coordinated within the implantation window that constitute essential elements in the repertoire that signifies endometrial receptivity and is aimed to achieve a better understanding of its relationship to fertility, infertility, and for the development of targeted antifertility agents for human use and welfare.

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Full-Length Complementary DNA and the Derived Amino Acid Sequence of Horse Uteroglobin1

Cited by 17 publications

References 32 publications

Multiple secretoglobin 1A1 genes are differentially expressed in horses

Multiple secretoglobin 1A1 genes are differentially expressed in horses

IFN-γ Stimulates the Expression of a Novel Secretoglobin That Regulates Chemotactic Cell Migration and Invasion

Endometrial receptivity: Clinical assessment in relation to fertility, infertility, and antifertility

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