Full-Length Genome Sequence of a Novel European Antigenic Variant Strain of Infectious Bursal Disease Virus

Molinet, Annonciade; Courtillon, Céline; Men, Maryvonne Le; Amenna-Bernard, Nadia; Retaux, Charlotte; Leroux, Aurélie; Lucas, Pierrick; Blanchard, Yannick; Nooruzzaman, Mohammed; Islam, Mohammad Rafiqul; Briand, François-Xavier; Grasland, Béatrice; Eterradossi, Nicolas; Soubies, Sébastien Mathieu

doi:10.1128/mra.00102-22

Cited by 2 publications

(2 citation statements)

References 12 publications

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“…A similar situation is observed across Europe, hitherto dominated by vvIBDV, and now facing a wave of A3B1 IBDV infections [ 18 , 19 ]. Furthermore, the occurrence of a new A9B1 genotype was shown in Portugal [ 13 ] and antigenic variants in France [ 35 ]. The present study aimed to update the epidemiological situation of IBDV in Poland in order to detect potential threats to domestic poultry production.…”

Section: Discussionmentioning

confidence: 99%

Epidemiology of Infectious Bursal Disease Virus in Poland during 2016–2022

Pikuła

Lisowska

Domańska-Blicharz

2023

Viruses

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Infectious bursal disease virus is an immunosuppressive ubiquitous pathogen that causes serious economic losses in poultry production. The virus is prone to genetic changes through mutations and reassortment, which drive the emergence of new variants and lead to a change in the epidemiological situation in a field. Such a situation is currently being reported due to a large wave of IBDV A3B1 reassortant infections in northwestern Europe. On the other hand, in Poland, which is the largest producer of chicken meat in the EU, the IBDVs of genotypes A3B2 and A3B4 were circulating just before the emergence of A3B1 reassortants. The purpose of the presented study was to update the IBDV epidemiological situation. The performed molecular survey based on the sequence of both genome segments showed the presence of very virulent strains (A3B2) and reassortants of genotypes A3B4 and A3B1; moreover, two of these genotypes are newly introduced IBDV lineages. In addition, a number of amino acid substitutions were demonstrated, including within antigenic epitopes and virulence determinants. In conclusion, the results obtained indicated a dynamic epidemiological situation in Poland, which highlights the need for further monitoring studies in the region and verification of protection conferred by the vaccines used against infection with detected IBDV.

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Section: Discussionmentioning

confidence: 99%

Epidemiology of Infectious Bursal Disease Virus in Poland during 2016–2022

Pikuła

Lisowska

Domańska-Blicharz

2023

Viruses

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“…Furthermore, the data used to supply the machine learning algorithms contained a limited number of strains for each pathotype. Including more variant and reassortant IBDV strains would enhance the model relevance regarding the pathotyping of recently appeared strains of that kind [ 50 – 52 ].…”

Section: Discussionmentioning

confidence: 99%

Infectious bursal disease virus: predicting viral pathotype using machine learning models focused on early changes in total blood cell counts

Molinet,

Courtillon,

Bougeard

et al. 2023

Vet Res

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Infectious bursal disease (IBD) is an avian viral disease caused in chickens by infectious bursal disease virus (IBDV). IBDV strains (Avibirnavirus genus, Birnaviridae family) exhibit different pathotypes, for which no molecular marker is available yet. The different pathotypes, ranging from sub-clinical to inducing immunosuppression and high mortality, are currently determined through a 10-day-long animal experiment designed to compare mortality and clinical score of the uncharacterized strain with references strains. Limits of this protocol lie within standardization and the extensive use of animal experimentation. The aim of this study was to establish a predictive model of viral pathotype based on a minimum number of early parameters measured during infection, allowing faster pathotyping of IBDV strains with improved ethics. We thus measured, at 2 and 4 days post-infection (dpi), the blood concentrations of various immune and coagulation related cells, the uricemia and the infectious viral load in the bursa of Fabricius of chicken infected under standardized conditions with a panel of viruses encompassing the different pathotypes of IBDV. Machine learning algorithms allowed establishing a predictive model of the pathotype based on early changes of the blood cell formula, whose accuracy reached 84.1%. Its accuracy to predict the attenuated and strictly immunosuppressive pathotypes was above 90%. The key parameters for this model were the blood concentrations of B cells, T cells, monocytes, granulocytes, thrombocytes and erythrocytes of infected chickens at 4 dpi. This predictive model could be a second option to traditional IBDV pathotyping that is faster, and more ethical.

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Full-Length Genome Sequence of a Novel European Antigenic Variant Strain of Infectious Bursal Disease Virus

Abstract: We report the full-length genome sequence (compared to reference sequences) of a novel European variant strain of infectious bursal disease virus (IBDV), designated 19P009381 (AxB1). This should help to further identify such viruses in Europe.

Cited by 2 publications

References 12 publications

Epidemiology of Infectious Bursal Disease Virus in Poland during 2016–2022

Epidemiology of Infectious Bursal Disease Virus in Poland during 2016–2022

Infectious bursal disease virus: predicting viral pathotype using machine learning models focused on early changes in total blood cell counts

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