Full-length sequence of a hepatitis C virus genome having poor homology to reported isolates: Comparative study of four distinct genotypes

Okamoto, Hiroaki; Kurai, Kiyohiko; Okada, Shunichi; Yamamoto, Keijiro; Lizuka, Hisao; Tanaka, Takeshi; Fukuda, Satoko; Tsuda, Fumio; Mishiro, Shunji

doi:10.1016/0042-6822(92)90762-e

Cited by 552 publications

(314 citation statements)

References 23 publications

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“…1, were tailed with dATP or dTTP homopolymer by a terminal deoxynucleotidyl transferase (Boehringer Mannheim), and amplified by single-sided PCR with 43-mer oligonucleotides containing (T) "( or (A) "( following the method described previously (Okamoto et al, 1992). For the GT230 isolate, 27 clones were obtained by poly(T) tailing and 34 by poly(A) tailing.…”

Section: Methodsmentioning

confidence: 99%

“…1, was attempted by single-sided PCR (Okamoto et al, 1992). However, no sequences were amplifiable by PCR with sense primers specific for GT230 or GT110 and the 20-mer primer (F166), representing a part of the 43-mer oligonucleotide (Okamoto et al, 1992), on cDNA that had been reverse-transcribed from extracted RNAs with the 43-mer oligonucleotide with either (T) "( or (A) "( . Therefore, the 3h ends of extracted RNAs were tailed with poly(A) by poly(A) polymerase (TaKaRa Biochemicals), reverse-transcribed with a 43-mer oligonucleotide containing (T) "( , and then amplified by the single-sided PCR.…”

Section: Methodsmentioning

confidence: 99%

“…PCR products were separated by gel electrophoresis and cloned into M13 phage vectors by the method described previously (Okamoto et al, 1992). Nucleotide sequences were determined for each cDNA clone on both plus and minus strands by ALF AutoRead DNA sequencing kits (Pharmacia) and Thermo Sequenase fluorescent-labelled primer cycle sequencing kit with 7-deaza-dGTP (Amersham).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

The entire nucleotide sequences of two GB virus C/hepatitis G virus isolates of distinct genotypes from Japan.

Okamoto

Nakao

Inoue

et al. 1997

Journal of General Virology

100

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Recently, putative viral agents responsible for human non-A to E hepatitis have been independently reported by two groups of investigators and designated GB virus C (GBV-C) and hepatitis G virus (HGV), respectively. The entire nucleotide sequences were determined for two viral genomes isolated from Japanese blood donors with GBV-C RNA. One of them (GT230) had a total genomic length of 9390 nucleotides (nt) with 5h and 3h untranslated regions of 551 and 313 nt, while the other (GT110) had genomic lengths of 9395, 281 and 315 nt, respectively. They both had a single long open reading frame, encoding 2842 amino acids (aa) in GT230 and 2933 aa in GT110. Surprisingly, they both lacked a clearly identifiable core gene, and possessed the E1/E2 gene with only four potential

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

The entire nucleotide sequences of two GB virus C/hepatitis G virus isolates of distinct genotypes from Japan.

Okamoto

Nakao

Inoue

et al. 1997

Journal of General Virology

100

View full text Add to dashboard Cite

show abstract

“…HCV has a positivestranded RNA as its genome. Nucleotide sequence analyses of HCV clones isolated in different places in the world revealed that there are significant sequence diversities (8)(9)(10)(11)(12)(13)(14)(15). The analysis of the region of non-structure 5 (NS5) of the HCV genome found, at least five different HCV genotypes in the world (16).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Hepatitis C Virus Strains Obtained From Hemodialysis Patients

Chou

Lin

Sheu

et al. 1993

JJMSB

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“…Tanto o HCV como o HIV podem ser subdividos em diferentes genótipos e subtipos, que apresentam diferentes níveis de divergência das seqüências originais do vírus, dependendo da região genômica estudada, resultantes de erros durante a replicação viral causadas pela ausência de atividade de reparo da enzima RNA polimerase [11][12][13] .…”

Section: Introductionunclassified

Co-infecção HIV/HCV em pacientes de Botucatu e região

Corvino

Henriques

Grotto

et al. 2007

Rev. bras. epidemiol.

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Devido à similaridade nas rotas de transmissão, a co-infecção HIV/HCV é freqüente, afetando em média 30 a 50% dos portadores de HIV. O presente estudo visou avaliar uma possível associação entre os subtipos do HIV e genótipos do HCV em pacientes co-infectados, com base na análise das freqüências em pacientes mono e co-infectados. Para determinação da freqüência dos subtipos HIV e genótipos HCV, foram analisados respectivamente 124 e 496 pacientes mono-infectados. O estudo da co-infecção foi realizado num grupo de 150 pacientes HIV positivos e esteve presente em 22 (14,7%) dos pacientes. A freqüência dos subtipos do HIV-1 em mono-infectados foi: subtipo B (85,5%), subtipo F (12,9%) e recombinante B/F (1,6%), enquanto nos genótipos HCV foi: 1a (25%), 1b (29,4%), 1a/1b (3,6%), 3a (35%), 2 (1,8%) e 5 (0,4%). Nos co-infectados o padrão de distribuição dos subtipos HIV-1 é semelhante aos mono-infectados, ou seja, subtipo B (85,0%), seguido do subtipo F (15,0%). A distribuição de freqüência de genótipos HCV nos co-infectados foi: 1a (36,3%), 1b (27,3%), 1a/1b (9,1%) e 3a (27,3%) mostrando um aumento de 10% na freqüência do genótipo 1, queda de 7,7% no genótipo 3 e ausência de outros genótipos. A análise estatística de associação entre os subtipos HIV e genótipos HCV (Goodman) mostrou que no genótipo 1 (HCV) ocorreu predominância do subtipo B, enquanto no genótipo 3 (HCV) a distribuição dos subtipos B e F (HIV-1) foi casual. Isto aponta para a necessidade de mais estudos desse grupo e um maior valor amostral.

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Full-length sequence of a hepatitis C virus genome having poor homology to reported isolates: Comparative study of four distinct genotypes

Cited by 552 publications

References 23 publications

The entire nucleotide sequences of two GB virus C/hepatitis G virus isolates of distinct genotypes from Japan.

The entire nucleotide sequences of two GB virus C/hepatitis G virus isolates of distinct genotypes from Japan.

Comparison of Hepatitis C Virus Strains Obtained From Hemodialysis Patients

Co-infecção HIV/HCV em pacientes de Botucatu e região

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