In recent years, the assessment of the shear resistance of existing post‐tensioned multispan bridges has become a frequent task for civil engineers. New models developed for this purpose need to be verified, since the number of shear‐related results of tests executed under real loading conditions at the interior support of multispan systems is very limited. In this study, eight shear tests carried out on four specimens are presented. The effects of different prestressing grades, cross‐sections, and amounts of transverse reinforcement are investigated. Two test setups were used in order to reproduce the internal forces occurring at the interior and end supports, respectively, of actual multispan bridges. With the aid of close‐range photogrammetry, detailed measurements of the kinematics of the critical flexural‐shear cracks were performed. In this study, it is demonstrated that arching action plays a major role for the shear capacity of slender post‐tensioned beams. The predictions of the shear capacity determined with various shear design models show that these models are conservative, especially with respect to the shear capacity for the region around the interior supports. However, the evaluation of the models also shows that the flexural‐shear crack model yields very promising results of the shear capacity of post‐tensioned beams with low shear reinforcement ratios.