2022
DOI: 10.1101/2022.12.22.521222
|View full text |Cite
Preprint
|
Sign up to set email alerts
|

Full site-specific addressability in DNA origami-templated silica nanostructures

Abstract: DNA nanotechnology allows for the fabrication of nano-meter-sized objects with high precision and selective addressability as a result of the programmable hybridization of complementary DNA strands. Such structures can template the formation of other materials, including metals and complex silica nanostructures, where the silica shell simultaneously acts to protect the DNA from external detrimental factors. However, the formation of silica nanostructures with site-specific addressability has thus far not been … Show more

Help me understand this report
View published versions

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

0
4
0

Year Published

2023
2023
2023
2023

Publication Types

Select...
2

Relationship

1
1

Authors

Journals

citations
Cited by 2 publications
(4 citation statements)
references
References 43 publications
0
4
0
Order By: Relevance
“…Finally, we also tested our lyophilization procedure on silicified DNA origami samples. DNA origami-templated silica nanostructures have recently gained more and more attention due to their excellent combination of properties of both the DNA origami and the inorganic silica component. ,,, They could present excellent candidates for in vivo applications due to their high stability and excellent biocompatibility. Therefore, the ability to form such structures and store them at high concentrations without an onset of aggregation is highly desirable.…”
Section: Resultsmentioning
confidence: 99%
See 2 more Smart Citations
“…Finally, we also tested our lyophilization procedure on silicified DNA origami samples. DNA origami-templated silica nanostructures have recently gained more and more attention due to their excellent combination of properties of both the DNA origami and the inorganic silica component. ,,, They could present excellent candidates for in vivo applications due to their high stability and excellent biocompatibility. Therefore, the ability to form such structures and store them at high concentrations without an onset of aggregation is highly desirable.…”
Section: Resultsmentioning
confidence: 99%
“…Therefore, the ability to form such structures and store them at high concentrations without an onset of aggregation is highly desirable. We employed our previously established silicification protocol ,, to 24HB and 4LB samples including a purification step after the silicification. The purified samples were diluted to 10 nM in ultrapure water, shock-frozen in liquid nitrogen, lyophilized, and resuspended to high concentrations as before.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…To realize such measurements, the nanosensors could be further protected by a layer of silica to preserve their integrity in cells. Importantly, site-specific silanization of DNA origami has already been demonstrated 63,64 , showing that it is indeed possible to selectively protect nanoantennas without hindering the binding of analyte molecules to be detected.…”
Section: -1250mentioning
confidence: 99%