Fully-automated and field-deployable blood leukocyte separation platform using multi-dimensional double spiral (MDDS) inertial microfluidics

Jeon, Hyungkook; Jundi, Bakr; Choi, Kyungyong; Ryu, Hyunryul; Levy, Bruce D.; Lim, Geunbae; Han, Jongyoon

doi:10.1039/d0lc00675k

Cited by 58 publications

(77 citation statements)

References 71 publications

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“…Inertial microfluidics as a size-based separation method is suitable for isolating WBCs from blood samples owing to the relatively distinct size difference in different blood cell types. Many previous studies utilized inertial microfluidic designs to directly sort WBCs from blood [30][31][32]. However, they only demonstrated sorting of 3 μm [31], 5 μm [30], or even 6 μm [32] diameter cells from 10 μm diameter ones regardless of the subpopulations of WBCs such as lymphocytes with an average size of only 7 μm.…”

Section: Introductionmentioning

confidence: 99%

“…Many previous studies utilized inertial microfluidic designs to directly sort WBCs from blood [30][31][32]. However, they only demonstrated sorting of 3 μm [31], 5 μm [30], or even 6 μm [32] diameter cells from 10 μm diameter ones regardless of the subpopulations of WBCs such as lymphocytes with an average size of only 7 μm. Considering the complete population of WBCs during blood assessment can reflect a more accurate heath situation [7].…”

Section: Introductionmentioning

confidence: 99%

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A low‐cost and high‐throughput benchtop cell sorter for isolating white blood cells from whole blood

Tayebi

2021

Electrophoresis

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The ability to isolate and purify white blood cells (WBCs) from mixed ensembles such as blood would benefit autologous cell-based therapeutics as well as diagnosis of WBC disorders. Current WBCs isolation methods have the limitations of low purity or requiring complex and expensive equipment. In addition, due to the overlap in size distribution between lymphocytes (i.e., a sub-population of WBCs) and red blood cells (RBCs), it is challenging to achieve isolation of entire WBCs populations. In this work, we developed an inertial microfluidics-based cell sorter, which enables size-based, high-throughput isolation, and enrichment of WBCs from RBC-lysed whole blood. Using the developed inertial microfluidic chip, the sorting resolution is sharpened within 2 μm, which achieved separation between 3 and 5 μm diameter particles. Thus, with the present cell sorter, a full population of WBCs can be isolated from RBC-lysed blood samples with recovery ratio of 92%, and merely 5% difference in the composition percentage of the three subpopulations of granulocytes, monocytes, and lymphocytes compared to the original sample. Furthermore, our cell sorter is designed to enable broad application of size-based inertial cell sorting by supplying a series of microchips with different sorting cutoff size. This strategy allows us to further enrich the lymphocytes population by twofold using another microchip with a cutoff size between 10 and 15 μm. With simplicity and efficiency, our cell sorter provides a powerful platform for isolating and sorting of WBCs and also envisions broad potential sorting applications for other cell types.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A low‐cost and high‐throughput benchtop cell sorter for isolating white blood cells from whole blood

Tayebi

2021

Electrophoresis

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“…2a) in the original PDMS MDDS device and the plastic MDDS device, respectively, under the same flow rate condition of 9.2 mL min −1 (2.3 mL min −1 ×4 for the quad-version); 2.3 mL min −1 is the optimized flow rate condition of the original PDMS MDDS device for blood separation as well as particle separation. 39 The results clearly show that the plastic MDDS device has a smaller dimension than the actual dimension of the original PDMS device that is deformed under pressure. Particles experienced a higher lift force so that the trajectory of 6 μm particles was shifted closer to the inner wall compared to the PDMS device.…”

Section: Analysis Of Channel Deformation In the Pdms Spiral Devicesmentioning

confidence: 94%

“…To overcome the limitations in the PDMS device, we developed deformation-free and mass-producible plastic inertial microfluidic systems (Fig. 1c), where we previously optimized PDMS inertial devices for blood separation 39 and Chinese hamster ovary (CHO) cell retention, [40][41][42] respectively. In inertial microfluidics, the channel dimension (especially the height of the channel) is a critical parameter determining the particle focusing in the channel by affecting particleexperienced forces (lift and Dean drag forces).…”

Section: Overview Of the Plastic Spiral Inertial Microfluidic Devicementioning

confidence: 99%

“…The first spiral device translated to the plastic device is the multi-dimensional double spiral (MDDS) device designed for leukocyte isolation from the blood sample. 39 The MDDS device consists of sequentially connected two spiral channels having different dimensions to integrate two different functions, sample focusing and separation, in a single device without sheath flow (Fig. 2a).…”

Section: Analysis Of Channel Deformation In the Pdms Spiral Devicesmentioning

confidence: 99%

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Engineering a deformation-free plastic spiral inertial microfluidic system for CHO cell clarification in biomanufacturing

et al. 2022

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Microfluidic Label‐Free Hydrodynamic Separation of Blood Cells: Recent Developments and Future Perspectives

Lee

Kim

Yang

2023

Adv Materials Technologies

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The biophysical characteristics and counts of blood cells provide useful information regarding pathological conditions. Therefore, the separation of blood components is a crucial task in biology, clinical diagnosis, therapeutics, and personalized medicine. Recently, microfluidics has gained significant interest as an effective technology for separating target cells from heterogeneous cell populations. Within cellular-scale microchannels, cells of interest are separated based on intrinsic properties, such as size, shape, and deformability, followed by further downstream or off-chip analysis. In this review, microfluidic-based hydrodynamic cell-separation technologies used in label-free approaches by categorizing them according to their key working principles are discussed: i) flow fractionation, ii) deterministic lateral displacement, iii) hydrophoresis, iv) inertial migration, and v) microfiltration. An overview of the major separation mechanisms is provided, and the relative performances and features of these technologies are thoroughly discussed. In addition, future perspectives regarding microfluidic system commercialization and standardization are briefly provided for their widespread use and applications.

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Fully-automated and field-deployable blood leukocyte separation platform using multi-dimensional double spiral (MDDS) inertial microfluidics

Abstract: A fully-automated and portable leukocyte separation platform was developed based on a new type of inertial microfluidic device, multi-dimensional double spiral (MDDS) device, as an alternative to centrifugation. By combining...

Cited by 58 publications

References 71 publications

A low‐cost and high‐throughput benchtop cell sorter for isolating white blood cells from whole blood

A low‐cost and high‐throughput benchtop cell sorter for isolating white blood cells from whole blood

Engineering a deformation-free plastic spiral inertial microfluidic system for CHO cell clarification in biomanufacturing

Microfluidic Label‐Free Hydrodynamic Separation of Blood Cells: Recent Developments and Future Perspectives

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