Fully automated assay for the determination of sumatriptan in human serum using solid-phase extraction and high-performance liquid chromatography with electrochemical detection

High performance liquid chromatographic method for the determination of sumatriptan with fluorescence detection in human plasma

“…In this study, fluorescence detection was used instead of coulometric detection [5][6][7][8] or mass spectrometry detection [9,10]. This mode of detection is simple, stable, robust and gave a similar limit of quantitation (1 ng/ml when using 0.5 ml of plasma) to those previously published methods.…”

“…Among the methods described in the literature, there is high performance liquid chromatography (HPLC) with coulometric detection [5][6][7][8] and HPLC with mass spectrometric detection [9][10][11]. In these methods, the sumatriptan was extracted from plasma samples using liquid-liquid extraction or solid phase extraction.…”

Challenges in the simultaneous quantitation of sumatriptan and naproxen in human plasma: Application to a bioequivalence study

“…Several methods are reported for the determination of SUM in biological matrices by HPLC with fluorescence [9,10], electrochemical [11][12][13], ultraviolet detection [14,15] and LC-MS/MS [16][17][18][19][20][21][22]. In majority of these published methods, the sensitivity was ≥1 ng/mL [9][10][11][12]16,20,22] with high volume of biological fluid (≥0.5 mL) for sample processing [9][10][11][12][13]16,17,[19][20][21].…”

Analysis of CP-122,288 at femto-molar levels from human plasma using solid-supported extraction and HPLC column switching

“…Other assays for compounds of this class such as Sumatriptan [2] have been developed using electrochemical detection, the presence of the indole moiety in the compound facilitates this [3]. HPLC with electrochemical detection was investigated for CP-122,288 in plasma but even with extensive sample preparation techniques, chemical noise and baseline instability compromised the robustness of such an assay approach.…”