Fully automated method for the determination of 24,25(OH)2 and 25(OH) D3 hydroxyvitamins, and Vitamins A and E in human serum by HPLC

An optimized and validated RP-HPLC/UV detection method for simultaneous determination of all-trans-Retinol (Vitamin A) and α-Tocopherol (Vitamin E) in human serum: Comparison of different particulate reversed-phase HPLC columns

“…This is due to the short-lived nature of reactive oxygen/nitrogen species formed and lake of well-established methods for measuring oxidative stress status in humans [17]. Although several methods for the determination of these vitamins in plasma and serum have been reported, information on their analytical quality is lacking [18][19][20][21][22][23]. To solve this discordance the accurate measurement of serum concentration of retinol and ␣-tocopherol is an important issue [4].…”

Voltammetric analysis of DL‐α‐tocopherol with a paste electrode

“…1 A sensitive, simple yet low-cost analytical technique is necessary to determine the amount of VE in food, medicines and other biological substances. Various analytical techniques have been devised, including high-performance liquid chromatography (HPLC), 2 -4 HPLC-fluorescence detection and HPLC-MS, 5,6 LC with electrochemical Coulometric detection, 7 spectrophotometric methods, 8 LC with fluorescence 9 -11 and gas chromatography LC-MS. 12 All these systems approach sensitive concentration ranges.…”

HPLC method for simultaneous determination of retinoids and tocopherols in human serum for monitoring of anticancer therapy

“…Plasma was extracted with tert-butyl methyl ether, using Extrelut columns, followed by a second clean-up on amino columns [13]. Vitamin E was extracted with other vitamins using a robotic station for liquid -liquid extraction, connected on-line with an automatic system for solid-phase extraction [38]. iological and pharmacological concentrations of retinoids and tocopherols in serum samples is presented.…”