Abstract:Peptide ligands are
widely used in protein purification by affinity
chromatography. Here, we applied a fully automated two-stage library
screening method that avoids false positive peptidyl-bead selection
and applied it to tetanus toxoid purification. The first library screening
was performed using only sulforhodamine (a fluorescent dye), and fluorescent
beads were isolated automatically by flow cytometry and discarded.
A second screening was then performed with the rest of the library,
using the target protei… Show more
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.