Effective wound care management system demands the emergence
of
smart bandages with wound healing techniques. Electrical stimulation
(ES)-based wound healing has shown excellent results in faster wound
closure by mimicking the endogenous electric field naturally produced
at the sight of a wound and assisting the same. The present work highlights
the development of a flexible copper-based micro-electrode array (MEA)
patch over a polyimide substrate for triggering ES-induced accelerated
wound healing for applied DC potentials only. Simulation results highlight
the ES optimization for varying maximum DC potentials of 0.2, 0.4,
and 1.0 V toward the generation of effective electric field-induced
electrotaxis. A simulated patch was fabricated using a unique screen-printed
masking technique involving the use of polyvinyl chloride (PVC) ink
as a masking material over a copper polyimide film to realize the
MEA structures. An integrated pH sensor was also fabricated using
screen printing of Ag/AgCl ink over the same to monitor in situ blood
pH levels. The developed low-cost MEA patches showed good electrical
continuity as well as excellent flexibility and skin conformality.
It was thereafter mounted over an 8 mm diameter cutaneous wound on
a rat model, and appropriate ES was applied as per the simulation
data. Post-healing results were visually verified as well as examined
for histological changes in tissue cross-sections, and an excellent
correlation between the simulation findings and visual observations
was obtained. A significant reduction in the healing time was achieved
(9 days) through this study for an optimized DC potential of 0.4 V,
unlike the control samples, which took 13 days for healing. The results
also mimicked the natural endogenous potential, thereby clearly demonstrating
the effectiveness of the fabricated patch toward accelerated wound
healing.