Fulvic acid supplementation and selenium deficiency disturb the structural integrity of mouse skeletal tissue. An animal model to study the molecular defects of Kashin-Beck disease

Yang, Chunlin; Niu, Congrong; Bodó, Michael; Gabriel, E.; Notbohm, Holger; Wolf, Eduard; Müller, P. K.

doi:10.1042/bj2890829

Cited by 64 publications

(36 citation statements)

References 19 publications

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“…To this point, as also suggested by the literature (14)(15)(16)(17), it is worth noting that a changed matrix is certainly relevant to the biological features of bone. Although the main difference between trabecular and cortical bone is the much greater surface area of the former, which explains the differences in bone turnover rates between cortical and trabecular bone, the hypothesis that a modified and possibly defective matrix could somehow be recognized and further removed by osteoclasts cannot be excluded.…”

Section: Discussionmentioning

confidence: 90%

17β-Estradiol and Tamoxifen Prevent the Over-Glycosylation of Rat Trabecular Bone Collagen Induced by Ovariectomy

Moro¹,

Bettica²,

Romanello³

et al. 1997

CCLM

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Summary:We have recently demonstrated that ovariectomy in the rat causes over-glycosylation of collagen which is restricted to trabecular bone. In order to obtain further evidence, we studied whether estrogen or tamoxifen treatment prevented over-glycosylation of trabecular bone collagen. Forty one-hundred-day-old female rats were subjected to ovariectomy (n = 30) or sham-operation (n = 10). Starting the day of the operation, sham-operated rats were treated with vehicle, while ovariectomized rats were divided into three groups and treated with vehicle (n = 10), estrogen (n = 10) or tamoxifen (n = 10). Five rats from each group were sacrificed at 115 and 145 days of age. Femurs and tibiae were separated into cortical and trabecular bone, demineralized, hydrolyzed and analyzed by HPLC for hydroxylysine glycosides and hydroxyproline content. Hydroxylysine glycoside content was expressed as a molar ratio with hydroxyproline. The results can be summarized as follows: 1) cortical bone collagen glycosylation did not vary among the different groups;2) over-glycosylation of trabecular bone collagen observed in the ovariectomized rats was prevented by the administration of either 17ß-estradiol or tamoxifen.These data demonstrated that estrogens affect glycosylation of trabecular bone collagen.

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Section: Discussionmentioning

confidence: 90%

17β-Estradiol and Tamoxifen Prevent the Over-Glycosylation of Rat Trabecular Bone Collagen Induced by Ovariectomy

Moro¹,

Bettica²,

Romanello³

et al. 1997

CCLM

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“…Detailed studies have been carried out on the thermal stability of collagen molecules with altered posttranslational modification patterns due to mutations [19], alterations of the enzymes and cofactors involved [20,211 or excess reactive oxygen [22]. Studies on the thermally induced overmodified collagen I from cell cultures demonstrated that over-glycosylation could decrease the diameter of collagen fibrils formed in vitro [7].…”

Section: Discussionmentioning

confidence: 99%

Collagen II from articular cartilage and annulus fibrosus

Yang

Rui

Mosler

et al. 1993

European Journal of Biochemistry

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Collagen I1 was isolated and characterized from hyaline cartilage (articular cartilage) and fibrocartilage (annulus fibrosus). Collagen I1 from the latter tissue has a substantially higher degree of hydroxylation and glycosylation than that isolated from articular cartilage. The higher degree of posttranslational modification was associated with a slower electrophoretic mobility, a greater resistance to mammalian collagenase digestion and a higher thermal stability. An increase of glycosylation accelerates the initial steps in fibril formation of collagen molecules but slows down the following lateral growth. The newly formed aggregates of collagen I1 from annulus fibrosus consisted of fibrils with a smaller diameter.Based on its histological appearance, cartilage is classified into three tissue entities, i. e. hyaline, elastic and fibrocartilage. Hyaline cartilage covers the articular surface of bone and supports the tracheal tubes, larynx and ventral ends of the ribs. Fibrocartilage is a transitional form between hyaline cartilage and fibrous connective tissue. It occurs in the menisci of joints, in the annulus fibrosus of intervertebral discs and in the attachment sites of tendons onto the bones [l]. Collagen, the main structural protein in both tissues, exhibits a high degree of organization based on fibrillar aggregates.Several factors have been suggested to play a role in the control of fibrillogenesis, such as the presence of proteoglycans [2], the mode of procollagen processing [3], the interaction of different collagen types [4] and non-collagenous proteins including integrins p]. Furthermore, experimental evidence showed that the posttranslational modification of collagen molecules plays a crucial role in the stability of individual molecules as well as the stabilization of collagen fibrils. Specifically, hydroxylation of prolyl residues determines the thermal stability of the triple helix of collagen molecules. Hydroxylation of lysyl residues contributes to the crosslinking of collagen molecules which, in turn, contributes to the biomechanical strength of collagen fibrils [6]. Recent circumstantial evidence provides further support that glycosylation of hydroxylysyl residues may regulate the diameter of collagen fibrils [7, 81. In this study, an approach was made to compare collagen I1 from hyaline cartilage (articular cartilage) and fibrocartilage (annulus fibrosus). The analysis showed that the collagen I1 from annulus fibrosus (AFII) has a substantially higher level of hydroxylation and glycosylation than the collagen I1 from articular cartilage (ACII). In order to clarify the influ- ence of different levels of posttranslational modification on the nature of collagen I1 molecules, their electrophoretic migration, thermal stability and susceptibility to mammalian collagenase were investigated. Additionally, we studied the impact of the varying pattern of collagen I1 modifications on the dynamics of the self-assembly and the structure of the fibrils formed in vitro. MATERIALS AND METHODS Extractio...

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“…Mycotoxins are indeed known to induce lipid peroxidation, an effect that can be prevented by antioxidants [5,11,12]. In animal experiments, FA and selenium deficiency have been reported to be associated with degeneration of articular cartilage, modification of collagen I in bone and collagen II in cartilage, development of fibrocartilage at the articular surface and impaired formation of subchondral bone [14]. The object of this study was to identify the risk factors for KBD in Tibet and to estimate the relative importance of each factor.…”

Section: Introductionmentioning

confidence: 99%

Epidemiological support for a multifactorial aetiology of Kashin-Beck disease in Tibet

Suetens

Moreno‐Reyes

Chasseur

et al. 2001

International Orthopaedics (SICOT)

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We carried out a cross-sectional study in 12 rural villages in order to identify the risk factors for Kashin-Beck disease in Tibet. Children aged 5-15 years (n=575) were examined and their corresponding houses were visited. Samples were collected in order to study fungal contamination of stored grain and the organic matter content of drinking water. Multivariate analysis was performed using logistic regression and population attributable fractions were computed to estimate the impact of each factor. The following variables were independently associated with the disease: age, gender, low socio-economic status, indicators of a poorly diversified diet, iodine deficiency and small water container size (with higher organic matter levels in small containers). Selenium deficiency was severe in all study subjects. The degree of fungal contamination of barley grain was related to the highest percentage of cases (65%) in a sample of the study population. Higher urinary iodine levels were not associated with decreasing prevalence rates when Alternaria sp. was isolated. The data that we report supports the hypothesis that Kashin-Beck disease occurs as a consequence of oxidative damage to cartilage and bone cells when associated with decreased antioxidant defence. Another mechanism that may coexist is bone remodelling stimulated by thyroid hormones whose actions can be blocked by certain mycotoxins.Résumé Nous avons réalisé une enquête transversale dans 12 villages ruraux afin d'identifier les facteurs de risque de la maladie de Kashin-Beck au Tibet. Les enfants âgés de 5 à 15 ans (n=575) et leurs maisons ont été examinés. La contamination fongique des céréales et le contenu en matières organiques de l'eau de boisson ont été étudiés dans des échantillons de la même population. L'analyse multivariée a été effectuée par régression logistique et les fractions attribuables ont été calculées afin d'estimer l'impact de chaque facteur. Les variables suivantes étaient indépendamment associées à la maladie: l'âge, le sexe, un bas état socio-économique, une alimentation peu diversifiée, la déficience en iode et une petite taille du réservoir à eau (présentant un contenu en matiè-res organiques plus élevé). La déficience en sélénium était sévère chez tous les enfants de l'étude. Le degré de contamination fongique des grains d'orge expliquait le plus haut pourcentage de cas (65%) dans le sous-modèle mycologique. L'iode urinaire n'était pas associé à la maladie lorsque Alternaria sp. était isolé. Les résultats sont compatibles avec des dommages oxydatifs aux cellules osseuses en présence d'une défense antioxidante défi-ciente. Un autre mécanisme co-existant possible est le remodelage osseux de l'os stimulé par les hormones thyroidiennes qui peut être bloqué par certaines mycotoxines.

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Fulvic acid supplementation and selenium deficiency disturb the structural integrity of mouse skeletal tissue. An animal model to study the molecular defects of Kashin-Beck disease

Cited by 64 publications

References 19 publications

17β-Estradiol and Tamoxifen Prevent the Over-Glycosylation of Rat Trabecular Bone Collagen Induced by Ovariectomy

17β-Estradiol and Tamoxifen Prevent the Over-Glycosylation of Rat Trabecular Bone Collagen Induced by Ovariectomy

Collagen II from articular cartilage and annulus fibrosus

Epidemiological support for a multifactorial aetiology of Kashin-Beck disease in Tibet

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