Fumonisin B1 induces oxidative stress in oesophageal (SNO) cancer cells

Khan, René; Phulukdaree, Alisa; Chuturgoon, Anil A.

doi:10.1016/j.toxicon.2017.12.041

Cited by 42 publications

(17 citation statements)

References 45 publications

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“…The present results showed that repeated daily exposure (per os) to FB1 led to increased jejunal mitochondrial levels of malondialdehyde (MDA), conjugated dienes (CD), and carbonyl proteins (CP), and decreased levels of mitochondrial catalase (CAT, key anti-oxidant enzyme) and total protein sulfhydryl (PSH, re ecting primarily the anti-oxidant glutathione) levels in a mouse model. These ndings of increases in mitochondrial oxidative stress were in accordance with previous studies of FB1 toxicity (Theumer et al 2010;Domijan and Abramov 2011;Abbès et al 2016;Khan et al 2018). One possible explanation for the presence of these stress/oxidative markers was an over-production of reactive oxygen species (ROS) secondary to a blockage in mitochondrial membrane potentials (Domijan and Abramov 2011).…”

Section: Discussionsupporting

confidence: 92%

Lactobacillus Paracasei BEJ01 Protects Against Fumonisin B1-Induced Intestinal Toxicity in Balb/c Mice

Ezdini

Salah-Abbès

Belgacem

et al. 2021

Preprint

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Fumonisin B1 (FB1) is a carcinogenic (Class 2B) mycotoxin produced by Fusarium fungi and is responsible for several types of mycotoxicoses in animals and humans. Lactobacillus paracasei (LP), as a probiotic, is known to impart a wide range of advantageous effects on host health. The objective of the current study was to evaluate if LP (type BEJ01) isolated from Tunisian artisanal butter, could potentially help protect a host against intestinal alterations caused by exposure to FB1. Here, adult male Balb/c mice were randomly assigned to four groups, i.e., control (vehicle only), treated with FB1 (100 µg/kg BW), LP (2 × 109 CFU/ml [≈ 2 mg/kg BW]) and FB1 (100 µg/kg BW) + LP (2 × 109 CFU/ml) and treated per os daily for 10 days. At 24 hr after the final treatment, mice were euthanized and their jejunum harvested for examination of intestinal alterations induced by the FB1. The data showed that a variety of negative effects in jejunal tissue were induced by the FB1, including DNA fragmentation, oxidative stress, apoptotic cell death, and histopathological alterations. The results also showed that co-treatment of LP with FB1 was able to mitigate the harmful FB1 effects. LP alone imparted no damage to jejunal tissues. These results show that apart from potential use as a bio-preservative for extending the shelf-life of food and feeds, LP may also provide a benefit of helping prevent untoward effects from a potent mycotoxin like FB1.

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Section: Discussionsupporting

confidence: 92%

Lactobacillus Paracasei BEJ01 Protects Against Fumonisin B1-Induced Intestinal Toxicity in Balb/c Mice

Ezdini

Salah-Abbès

Belgacem

et al. 2021

Preprint

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“…Initially, we evaluated the effects of FBs on GES-1 cells in many different ways. These were important indexes to evaluate the toxicity of fumonisin in many studies [ 10 , 22 , 46 ]. Our data showed that FBs could markedly inhibit cell proliferation and increase cell membrane permeability, cell mortality, and the expression level of ER stress markers in varying degrees.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Individual and Combined Toxicity of Fumonisin Mycotoxins in Human Gastric Epithelial Cells

Jia

Liu

et al. 2020

IJMS

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Fumonisin contaminates food and feed extensively throughout the world, causing chronic and acute toxicity in human and animals. Currently, studies on the toxicology of fumonisins mainly focus on fumonisin B1 (FB1). Considering that FB1, fumonisin B2 (FB2) and fumonisin B3 (FB3) could coexist in food and feed, a study regarding a single toxin, FB1, may not completely reflect the toxicity of fumonisin. The gastrointestinal tract is usually exposed to these dietary toxins. In our study, the human gastric epithelial cell line (GES-1) was used as in vitro model to evaluate the toxicity of fumonisin. Firstly, we found that they could cause a decrease in cell viability, and increase in membrane leakage, cell death and the induction of expression of markers for endoplasmic reticulum (ER) stress. Their toxicity potency rank is FB1 > FB2 >> FB3. The results also showed that the synergistic effect appeared in the combinations of FB1 + FB2 and FB1 + FB3. Nevertheless, the combinations of FB2 + FB3 and FB1 + FB2 + FB3 showed a synergistic effect at low concentration and an antagonistic effect at high concentration. We also found that myriocin (ISP-1) could alleviate the cytotoxicity induced by fumonisin in GES-1 cells. Finally, this study may help to determine or optimize the legal limits and risk assessment method of mycotoxins in food and feed and provide a potential method to block the fumonisin toxicity.

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“…Fumonisins traditionally associated with cereals and cereal-based products can induce both acute and chronic toxic effects in poultry and animals. Fumonisin-contaminated cereal-based products have increased the incidence of esophageal and liver cancer ( Khan et al, 2018 ; Arumugam et al, 2019 ) while causing neural discomforts in humans. In animals, fumonisin is associated with several mycotoxicoses, pulmonary edema in pigs, and decreased bodyweight, immune disorders, and liver sclerosis in poultry ( Pósa et al, 2014 ; Vendruscolo et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Probiotic and Antifungal Attributes of Levilactobacillus brevis MYSN105, Isolated From an Indian Traditional Fermented Food Pozha

et al. 2021

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The use of probiotics and antifungal capabilities of the lactic acid bacteria (LAB) isolated from different niches is a strategy to prepare functional cultures and biopreservatives for food/feed industries. In the present study, LAB strains isolated from an Indian traditional fermented food, Pozha, were evaluated for their probiotic properties and biocontrol potential. A total of 20 LAB isolates were selected from Pozha samples collected aseptically and screened for their antagonistic activity against Fusarium verticillioides. Among the bioactive isolates, Lacticaseibacillus brevis MYSN105 showed the highest antifungal activity in vitro, causing some morphological alterations such as damaged mycelia and deformed conidia. Cell-free supernatant (CFS) from L. brevis MYSN105 at 16% concentration effectively reduced the mycelial biomass to 0.369 g compared to 1.938 g in control. Likewise, the conidial germination was inhibited to 20.12%, and the seed treatment using CFS induced a reduction of spore count to 4.1 × 106 spores/ml compared to 1.1 × 109 spores/ml for untreated seeds. The internal transcribed spacer (ITS) copy number of F. verticillioides decreased to 5.73 × 107 and 9.026 × 107 by L. brevis MYSN105 and CFS treatment, respectively, compared to 8.94 × 1010 in control. The L. brevis MYSN105 showed high tolerance to in vitro gastrointestinal conditions and exhibited high adhesive abilities to intestinal epithelial cell lines. The comparative genome analysis demonstrated specific secondary metabolite region coding for bacteriocin and T3PKS (type III polyketide synthase) possibly related to survival and antimicrobial activity in the gut environment. Our results suggest that L. brevis MYSN105 has promising probiotic features and could be potentially used for developing biological control formulations to minimize F. verticillioides contamination and improve food safety measures.

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Fumonisin B1 induces oxidative stress in oesophageal (SNO) cancer cells

Cited by 42 publications

References 45 publications

Lactobacillus Paracasei BEJ01 Protects Against Fumonisin B1-Induced Intestinal Toxicity in Balb/c Mice

Lactobacillus Paracasei BEJ01 Protects Against Fumonisin B1-Induced Intestinal Toxicity in Balb/c Mice

Evaluation of the Individual and Combined Toxicity of Fumonisin Mycotoxins in Human Gastric Epithelial Cells

Probiotic and Antifungal Attributes of Levilactobacillus brevis MYSN105, Isolated From an Indian Traditional Fermented Food Pozha

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