FUN26 (Function Unknown Now 26) Protein from Saccharomyces cerevisiae Is a Broad Selectivity, High Affinity, Nucleoside and Nucleobase Transporter

Boswell-Casteel, Rebba C.; Johnson, Jennifer M.; Duggan, Kelli D.; Roe-Žurž, Zygy; Schmitz, Hannah; Burleson, Carter; Hays, Franklin A.

doi:10.1074/jbc.m114.553503

Cited by 14 publications

(46 citation statements)

References 56 publications

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“…Early characterization of ENTs was done in erythrocytes (54, 55), followed by mammalian tumor cell lines (56, 57), then Xenopus laevis oocytes (3), and this eventually lead to attempts of characterizing purified transporters (50–53, 58) – but the ENTs proved to be resistant to characterization in detergent solubilized, purified form. Only recently have reports been made that ENTs are amenable to heterologous expression, purification, and functional characterization (59, 60). …”

Section: Functional Characterization Of Mammalian Ent Proteinsmentioning

confidence: 99%

“…Transporters from A. thaliana display broad substrate selectivity and affinity (3 to 100 μM), function as substrate-proton symporters (with exception to AtENT7), and are insensitive to the inhibitors NMBPR, dilazep, and dipryidamol (128). In addition to these transporters, FUN26 (Function Unknown Now 26) from Saccharomyces cerevisiae was recently identified as a broadly selective, high-affinity, nucleoside and nucleobase transporter (59). FUN26 is not stimulated by a pH differential nor is it sensitive to NBMPR (59, 130).…”

Section: Functional Characterization Of Non-mammalian Ent Proteinsmentioning

confidence: 99%

“…In addition to these transporters, FUN26 (Function Unknown Now 26) from Saccharomyces cerevisiae was recently identified as a broadly selective, high-affinity, nucleoside and nucleobase transporter (59). FUN26 is not stimulated by a pH differential nor is it sensitive to NBMPR (59, 130). Like hENT3, FUN26 has also demonstrated limited ability to transport nucleotides, albeit with lower affinity than nucleosides or nucleobases (Boswell-Casteel, unpublished data).…”

Section: Functional Characterization Of Non-mammalian Ent Proteinsmentioning

confidence: 99%

“…Like hENT3, FUN26 has also demonstrated limited ability to transport nucleotides, albeit with lower affinity than nucleosides or nucleobases (Boswell-Casteel, unpublished data). Importantly, FUN26 and AtENT7 are the first ENTs to be functionally characterized in purified form (59, 60), and this marks a major advancement in efforts to obtain a molecular structure of an ENT protein.…”

Section: Functional Characterization Of Non-mammalian Ent Proteinsmentioning

confidence: 99%

“…ENT3 is expected to be glycosylated, but this is yet to be proved (65). Multiple studies have identified residues that affect substrate transport or inhibitor binding (8, 25, 36, 67, 82, 135–153), mitochondria targeting (144), and targeting to the plasma membrane (59, 142, 149, 150). A list of mutated residues with their extrapolated function is available in Table 2 for human ENTs and Table 3 for the non-mammalian ENTs.…”

Section: Mutagenesis Studies Of Entsmentioning

confidence: 99%

See 4 more Smart Citations

Equilibrative nucleoside transporters—A review

Boswell-Casteel

Hays

2016

Nucleosides, Nucleotides and Nucleic Acids

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173

155

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Equilibrative nucleoside transporters (ENTs) are polytopic integral membrane proteins that mediate the transport of nucleosides, nucleobases, and therapeutic analogs. The best-characterized ENTs are the human transporters hENT1 and hENT2. However, non-mammalian eukaryotic ENTs have also been studied (e.g., yeast, parasitic protozoa). ENTs are major pharmaceutical targets responsible for modulating the efficacy of more than 30 approved drugs. However, the molecular mechanisms and chemical determinants of ENT-mediated substrate recognition, binding, inhibition, and transport are poorly understood. This review highlights findings on the characterization of ENTs by surveying studies on genetics, permeant and inhibitor interactions, mutagenesis, and structural models of ENT function.

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Section: Functional Characterization Of Mammalian Ent Proteinsmentioning

confidence: 99%

Section: Functional Characterization Of Non-mammalian Ent Proteinsmentioning

confidence: 99%

Section: Functional Characterization Of Non-mammalian Ent Proteinsmentioning

confidence: 99%

Section: Functional Characterization Of Non-mammalian Ent Proteinsmentioning

confidence: 99%

Section: Mutagenesis Studies Of Entsmentioning

confidence: 99%

See 3 more Smart Citations

Equilibrative nucleoside transporters—A review

Boswell-Casteel

Hays

2016

Nucleosides, Nucleotides and Nucleic Acids

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173

155

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Vitamin requirements and biosynthesis in Saccharomyces cerevisiae

Perli

Wronska

Ortiz‐Merino

et al. 2020

Yeast

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Chemically defined media for yeast cultivation (CDMY) were developed to support fast growth, experimental reproducibility, and quantitative analysis of growth rates and biomass yields. In addition to mineral salts and a carbon substrate, popular CDMYs contain seven to nine B‐group vitamins, which are either enzyme cofactors or precursors for their synthesis. Despite the widespread use of CDMY in fundamental and applied yeast research, the relation of their design and composition to the actual vitamin requirements of yeasts has not been subjected to critical review since their first development in the 1940s. Vitamins are formally defined as essential organic molecules that cannot be synthesized by an organism. In yeast physiology, use of the term “vitamin” is primarily based on essentiality for humans, but the genome of the Saccharomyces cerevisiae reference strain S288C harbours most of the structural genes required for synthesis of the vitamins included in popular CDMY. Here, we review the biochemistry and genetics of the biosynthesis of these compounds by S. cerevisiae and, based on a comparative genomics analysis, assess the diversity within the Saccharomyces genus with respect to vitamin prototrophy.

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Less is more: Nutrient limitation induces cross-talk of nutrient sensing pathways with NAD+ homeostasis and contributes to longevity

Tsang

Lin

2015

Front. Biol.

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Nutrient sensing pathways and their regulation grant cells control over their metabolism and growth in response to changing nutrients. Factors that regulate nutrient sensing can also modulate longevity. Reduced activity of nutrient sensing pathways such as glucose-sensing PKA, nitrogen-sensing TOR and S6 kinase homolog Sch9 have been linked to increased life span in the yeast, Saccharomyces cerevisiae, and higher eukaryotes. Recently, reduced activity of amino acid sensing SPS pathway was also shown to increase yeast life span. Life span extension by reduced SPS activity requires enhanced NAD+ (nicotinamide adenine dinucleotide, oxidized form) and nicotinamide riboside (NR, a NAD+ precursor) homeostasis. Maintaining adequate NAD+ pools has been shown to play key roles in life span extension, but factors regulating NAD+ metabolism and homeostasis are not completely understood. Recently, NAD+ metabolism was also linked to the phosphate (Pi)-sensing PHO pathway in yeast. Canonical PHO activation requires Pi-starvation. Interestingly, NAD+ depletion without Pi-starvation was sufficient to induce PHO activation, increasing NR production and mobilization. Moreover, SPS signaling appears to function in parallel with PHO signaling components to regulate NR/NAD+ homeostasis. These studies suggest that NAD+ metabolism is likely controlled by and/or coordinated with multiple nutrient sensing pathways. Indeed, cross-regulation of PHO, PKA, TOR and Sch9 pathways was reported to potentially affect NAD+ metabolism; though detailed mechanisms remain unclear. This review discusses yeast longevity-related nutrient sensing pathways and possible mechanisms of life span extension, regulation of NAD+ homeostasis, and cross-talk among nutrient sensing pathways and NAD+ homeostasis.

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FUN26 (Function Unknown Now 26) Protein from Saccharomyces cerevisiae Is a Broad Selectivity, High Affinity, Nucleoside and Nucleobase Transporter

Cited by 14 publications

References 56 publications

Equilibrative nucleoside transporters—A review

Equilibrative nucleoside transporters—A review

Vitamin requirements and biosynthesis in Saccharomyces cerevisiae

Less is more: Nutrient limitation induces cross-talk of nutrient sensing pathways with NAD+ homeostasis and contributes to longevity

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