Function and culture requirements of snow leopard (Panthera uncia) spermatozoa in vitro

Roth, Terri L.; Howard, Jeremy; Donoghue, Ann M.; Swanson, William F.; Wildt, D. E.

doi:10.1530/jrf.0.1010563

Cited by 29 publications

(20 citation statements)

References 8 publications

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“…; n = 8. Results demonstrate that (a) PBS enhances (P < 0 05) sperm motility overall compared with Ham's F10 (data adapted from Roth et al 1994) and (b) sodium bicarbonate supplementation to PBS has a profound detrimental effect on sperm motility. cheetah (Donoghue et al 1992b), tiger (Donoghue et al 1992c) and clouded leopard ) are capable of binding and penetrating both the outer and inner layers of the domestic cat zona in vitro.…”

Section: Importance Of Species Specificity To the Wildlife Spermatolomentioning

confidence: 94%

“…It is interesting that snow leopard spermatozoa from high quality electroejaculates abruptly lose motility in Ham's F10 (Fig. 8a) (Roth et al 1994). In contrast, snow leopard spermatozoa exposed to simple phosphate-buffered saline (PBS) sustain a higher (P < 0 • 05) sperm motility index profile in vitro for at least 6 h. This unique medium preference indicates a species-specific sensitivity not observed among other felids studied to-date.…”

Section: Importance Of Species Specificity To the Wildlife Spermatolomentioning

confidence: 99%

“…Zona-binding and penetration did not differ (P>005) between media. Data adapted from Roth et al 1994. domestic cat and its wild relatives has been considered to occur readily in the presence of medium containing protein (Howard et al 1991a;Andrews et al 1992). Snow leopard sperm are different.…”

Section: Importance Of Species Specificity To the Wildlife Spermatolomentioning

confidence: 99%

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Spermatology for understanding, managing and conserving rare species

Wildt¹,

Pukazhenthi²,

Brown³

et al. 1995

Reprod. Fertil. Dev.

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Abstract. Most conventional spermatology research involves common mammalian species including livestock, laboratory animals and humans. Yet, there are more than 4500 mammalian species inhabiting the planet for which little is known about basic reproductive biology, including sperm characteristics and function. This information is important, not just as adjunct knowledge, but because the majority of these species are threatened with extinction, largely due to human-induced pressures. The field of conservation is changing rapidly, and global cooperation is emerging among a variety of wildlife enthusiasts, ranging from management authorities of nature reserves to curators of rare zoological collections. Conservation progress depends on systematic, multidisciplinary research first to answer basic questions, with new data then applied to endangered species management plans. The reproductive physiologist is a crucial component of this scheme. Reproduction is the essence of species survival, and enormous effort needs to be directed at these 'untraditionaP research species, subspecies and populations. Spermatology research combined with simultaneous efforts in endocrinology, embryology and cryopreservation (among others) can lead to the successful application of assisted reproduction. Examples from this laboratory include an array of wild fetid species and a rare cervid and mustelid. Obstacles to success are formidable, including unique species-specificities, diminished genetic diversity and a general lack of resources. Nonetheless, the field offers tremendous opportunities for generating unique knowledge of comparative interest and with conservation utility.

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Section: Importance Of Species Specificity To the Wildlife Spermatolomentioning

confidence: 94%

Section: Importance Of Species Specificity To the Wildlife Spermatolomentioning

confidence: 99%

Section: Importance Of Species Specificity To the Wildlife Spermatolomentioning

confidence: 99%

See 1 more Smart Citation

Spermatology for understanding, managing and conserving rare species

Wildt¹,

Pukazhenthi²,

Brown³

et al. 1995

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

show abstract

“…Captive snow leopards have been successfully immobilized with tiletamine-zolazepam (TZ; Roth et al, 1994), medetomidine (MED)-ketamine (Jalanka, 1989a), and a combination of xylazine and ketamine (Jalanka, 1989b). The three field studies that involved multiple immobilizations of wild snow leopards employed ketamine (Jackson, 1996), a combination of medetomidine and ketamine (Oli, 1997), and tiletamine-zolazepam (McCarthy et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Reversible Immobilization of Free-Ranging Snow Leopards (Panthera Uncia) With a Combination of Medetomidine and Tiletamine-Zolazepam

Johansson

Malmsten

Mishra

et al. 2013

Journal of Wildlife Diseases

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ABSTRACT:Conservation and research of the elusive snow leopard (Panthera uncia) have been hampered by inadequate knowledge about its basic life history. Global positioning system (GPS) collars can provide useful information, but there has been limited information available on safe capture methods, drug doses, and efficacy for effective immobilization of free-ranging snow leopards. We describe a drug protocol using a combination of medetomidine and tiletaminezolazepam for the chemical immobilization of free-ranging snow leopards. We also describe physiologic responses to immobilization drugs, including rectal temperature, heart rate, respiratory rate, and relative hemoglobin oxygen saturation (SpO 2 ) recorded every 10 min. Our study was carried out in the Tost Mountains adjacent to the Great Gobi Desert, in southern Mongolia, between August 2008 and April 2012. Eighteen snow leopards were captured or recaptured with foot-snares on 42 occasions and anesthetized for marking with GPS collars. The snow leopards received on average (6SD) 0.02060.04 mg/kg body mass medetomidine and 2.1760.45 mg/kg tiletamine-zolazepam. The duration of ensuing anesthesia was 69613 min, including an induction period of 10 (64) min. Anesthesia was reversed with 4 mg (0.1060.04 mg/ kg) atipamezole administered intramuscularly. The mean value for SpO 2 for the 37 captures where we could record physiologic values was 9164. The SpO 2 increased significantly during anesthesia (+0.0660.02%/min), whereas rectal temperature (average 38.160.7 C/min, change 20.0460.003 C/min), heart rate (average 9769 beats/min, change 20.2060.03 beats/min), and respiratory rate (average 2666 breaths/min, change 20.1160.03 breaths/min) decreased significantly. A dose of 80 mg tiletamine-zolazepam (2 mg/kg body weight) and 0.72 mg medetomidine (0.02 mg/kg body weight) safely immobilized all adult and subadult snow leopards (weight 25-45 kg) in our study. All measured physiologic values remained within clinically acceptable limits.

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“…Moreover, some researchers have reported successful semen collection from wild felids by using electroejaculation, such as tigers (Panthera tigris- Donoghue et al, 1992), snow leopards (Panthera uncia- Roth et al, 1994), Indian leopards (Panthera pardus- Jayaprakash et al, 2001), caracals (Caracal caracal- Pope et al, 2001), jaguars (Panthera onca- Morato et al, 2001;Silva et al, 2003), ocelots (Leopardus pardalis- Queiroz et al, 2002), margays (L. wiedii) and tigrinas (L. tigrinus- Morais et al, 2002). Graham et al (1978) reported the use of electroejaculation to collect semen from nondomestic felids.…”

Section: Electroejaculationmentioning

confidence: 99%