Function and heterogeneity of human Fc receptors for immunoglobulin G.

Unkeless, Jay C.

doi:10.1172/jci113891

Cited by 247 publications

(98 citation statements)

References 76 publications

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“…In particular, Ick is expressed in T-lymphoid cells and natural killer cells and to a lesser degree in some B cells (8), blk is expressed in B-lymphoid cells (20), fgr is expressed in normal monocytic cells and granulocytes (37), and hck is expressed in monocytes and granulocytes and at low levels in some B cells (41 in a signal transduction pathway common to myeloid cells and B-lymphoid cells. In this regard, it is interesting to note that several membrane molecules are expressed in a similar pattern on hematopoietic cells, including the receptors for complement components and the Fc portion of immunoglobulin molecules (31,33). Cross-linking of these cell surface molecules induces rapid tyrosine phosphorylation of several cellular proteins (31) protease digestion patterns were very similar for all of the proteins, demonstrating their identity and indicating that phosphorylation occurred on comparable sites in immunoprecipitates.…”

Section: Discussionmentioning

confidence: 93%

Hematopoietic cells express two forms of lyn kinase differing by 21 amino acids in the amino terminus.

Yi¹,

Bolen²,

Ihle³

1991

Mol. Cell. Biol.

146

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Section: Discussionmentioning

confidence: 93%

Hematopoietic cells express two forms of lyn kinase differing by 21 amino acids in the amino terminus.

Yi¹,

Bolen²,

Ihle³

1991

Mol. Cell. Biol.

146

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“…38 Fc␥RI (CD64) binds monomeric as well as aggregated human IgG with high affinity, whereas Fc␥RII (CD32) and Fc␥RIII (CD16) are receptors of lower affinity that only bind IgG in the form of ICs with a preference for IgG 1 and IgG 3 . 39,40 Immunohistochemical analysis has shown the presence of all three classes of Fc␥Rs in human atherosclerotic coronary arteries, where the majority of the receptor-bearing cells were of mononuclear phagocyte origin. 41 Our present results showed that Fc␥RI, and possibly also Fc␥RII are involved in the OxLDL-IgG IC-mediated monocyte survival.…”

Section: Discussionmentioning

confidence: 99%

OxLDL–IgG Immune Complexes Induce Survival of Human Monocytes

Oksjoki

Kovanen

Lindstedt

et al. 2006

ATVB

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Objective-Immune complexes containing oxidatively modified low-density lipoprotein (oxLDL) particles are deposited in human atherosclerotic lesions during atherogenesis. Here we studied whether OxLDL-IgG immune complexes (OxLDL-IgG ICs) affect survival of human monocytes. Methods and Results-As demonstrated by light microscopy, and analysis of cell proliferation, caspase-3 activity, and DNA fragmentation, OxLDL-IgG ICs promoted survival of cultured human monocytes by decreasing their spontaneous apoptosis. OxLDL-IgG ICs induced a concentration-dependent production of the major monocyte growth factor, monocyte colony-stimulating factor (M-CSF), by the monocytes, but its inhibition was without effect on OxLDL-IgG IC-induced monocyte survival. Rather, OxLDL-IgG ICs induced rapid phosphorylation of Akt, suggesting a direct anti-apoptotic effect mediated by cross-linking of Fc␥ receptors. Experiments with receptor blocking antibodies revealed that the OxLDL-IgG IC-induced monocyte survival was mediated by Fc␥ receptor I. Key Words: atherosclerosis Ⅲ monocytes Ⅲ oxLDL A therosclerosis is characterized by early accumulation of lipids and macrophages in the intimal layer of the arterial wall. Lipids, mainly derived from serum low-density lipoprotein (LDL) particles, accumulate in the extracellular matrix of the intima, were they are exposed to various modifications, such as oxidation. 1 The process may begin early in life as epitopes of oxLDL are found the aortic intima of human fetuses of hypercholesterolemic women, and this is followed by infiltration of monocytes in the intima. 2 OxLDL is recognized and taken up by macrophages via scavenger receptors, which are then transformed into lipid-filled foam cells. Modified lipids on the surface of oxLDL are biologically active and are recognized by various pattern recognition receptors of the innate immune system, notably by the Toll-like receptor 4 (TLR4). 3,4 OxLDL is also able to activate the adaptive immune system when antigen-presenting cells present peptides derived from oxLDL particle on MHC class II molecules for recognition by CD4 ϩ T cells, 5 which then leads to the production of antibodies specific for oxLDL. Consistently, human atherosclerotic lesions contain clones of CD4 ϩ T cells that recognize epitopes of oxLDL and respond to oxLDL stimulation by proliferation and cytokine production. 6 Moreover, antibodies against oxLDL are present in the circulation, and their levels have been shown to correlate positively with cardiovascular disease and its complications. [7][8][9] Human atherosclerotic lesions also contain IgG that recognizes oxLDL and OxLDL-IgG immune complexes (OxLDL-IgG IC). 10 When added to cultured macrophages, OxLDL-IgG ICs promote foam cell formation and induce the production of proinflammatory cytokines, oxygen radicals, and matrix metalloproteinases by the macrophages. [11][12][13] Infiltration of monocytes into the arterial intima involves their attachment to activated endothelium via vascular cell adhesion molecule (VCAM)-1, and transmigrat...

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“…In contrast to the high-af®nity CD64-mediated binding of monomeric IgG 1 and IgG 3 [13,14], the low-af®nity receptors CD32 and CD16 bind IgGs ef®ciently only in the form of antigen± antibody (Ag±Ab) complexes [5,13,15]. CD64 is expressed constitutively by monocytes and macrophages, and its expression in PMN occurs only upon activation [5,13,14]. CD32 is present on the plasma membrane of all professional phagocytes, as well as on some B cells, but it appears to respond poorly to activation [5,13,15].…”

Section: Introductionmentioning

confidence: 96%

“…CD64 is expressed constitutively by monocytes and macrophages, and its expression in PMN occurs only upon activation [5,13,14]. CD32 is present on the plasma membrane of all professional phagocytes, as well as on some B cells, but it appears to respond poorly to activation [5,13,15]. CD16 is found on professional phagocytes, eosinophils and natural killer (NK) cells and does not appear to respond to cell activation [5, 16±20].…”

Section: Introductionmentioning

confidence: 99%

Expression of Fcγr1 (CD64) on Polymorphonuclear Leucocytes during Progression to Acquired Immunodeficiency Syndrome in Perinatally Human Immunodeficiency Virus‐Infected Children

Moallem

Kalaycı

Homel³

et al. 2000

Scand J Immunol

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CD64, the high‐affinity receptor in the family of FCγ receptors, is not expressed constitutively in polymorphonuclear leucocytes (PMN). CD64 is expressed by PMNs in the late stages of human immunodeficiency virus (HIV) infection in adults. We followed the expression of CD64 on PMNs in perinatally HIV‐infected children during disease progression. Peripheral blood leucocytes (PBL) from 45 perinatally HIV‐infected paediatric patients and 13 healthy age‐matched controls were analysed using cytofluorimetry after reaction with a fluorophore‐labelled monoclonal antibody (MoAb) to CD64. In parallel, we examined the expression of CD32, CD16, CD11b and the human neutrophil‐specific BH2‐Ag using fluorophore‐labelled MoAbs. We found that up to 79.5% of the PMNs in children in class C3 express CD64. Most importantly, we observed a continuous and significant increase in the appearance of CD64+ PMNs as a function of CDC classification (P < 0.001) but no changes in the expression of CD32, CD16, CD11b and BH2‐Ag. This suggests that following the expression of CD64 on PMNs can be useful in evaluating the progression of HIV infection in perinatally HIV‐infected children.

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Function and heterogeneity of human Fc receptors for immunoglobulin G.

Cited by 247 publications

References 76 publications

Hematopoietic cells express two forms of lyn kinase differing by 21 amino acids in the amino terminus.

Hematopoietic cells express two forms of lyn kinase differing by 21 amino acids in the amino terminus.

OxLDL–IgG Immune Complexes Induce Survival of Human Monocytes

Expression of Fcγr1 (CD64) on Polymorphonuclear Leucocytes during Progression to Acquired Immunodeficiency Syndrome in Perinatally Human Immunodeficiency Virus‐Infected Children

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