Function and Regulation of the
            <i>Saccharomyces cerevisiae ENA</i>
            Sodium ATPase System

Ruiz, Amparo; Ariño, Joaquín

doi:10.1128/ec.00337-07

Cited by 103 publications

(109 citation statements)

References 102 publications

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“…The TOR (via Gln3/Ure2) (Crespo et al, 2001), Snf1 (via Mig1/Mig2 and Nrg1/Nrg2) (Platara et al, 2006), Rim101 (via Nrg1/Nrg2) (Lamb et al, 2001;Lamb & Mitchell, 2003;Platara et al, 2006) and the calcineurin pathways all converge on the ENA1 promoter (Cyert, 2003;Haro et al, 1991;Ruiz & Arino, 2007). The TOR and Snf1 pathways respond to nutrient availability, and the calcineurin pathway responds to intracellular ion concentrations, while Rim101 and Snf1 also regulate the yeast response to alkaline stress.…”

Section: (A) (B)mentioning

confidence: 99%

“…For example, treatment of yeast cells with 0.8 M NaCl results in the phosphorylation of Snf1 on Thr 210 , thereby activating the kinase (McCartney & Schmidt, 2001). When yeast cells are exposed to increasing concentrations of sodium ions, the cells respond by inducing the transcription of ENA1, which encodes a Na + /Li + efflux pump needed for sodium and lithium tolerance and growth in alkaline media (Cyert, 2003;Haro et al, 1991;Ruiz & Arino, 2007). Many signalling pathways affect the transcription of ENA1.…”

Section: Introductionmentioning

confidence: 99%

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Nsf1/Ypl230w participates in transcriptional activation during non-fermentative growth and in response to salt stress in Saccharomyces cerevisiae

et al. 2008

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Section: (A) (B)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nsf1/Ypl230w participates in transcriptional activation during non-fermentative growth and in response to salt stress in Saccharomyces cerevisiae

et al. 2008

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“…First, we measured the activation of the ENA1 promoter by means of a translational ENA1-lacZ fusion reporter. ENA1 encodes a Na + -ATPase (also able to pump out Li + ), which represents a major component for cation homeostasis and is essentially regulated at the transcriptional level by alkaline cation and high pH stresses (Ruiz and Arino 2007). As shown in Figure 4A, overexpression of Ypi1 results in increased expression from the ENA1 promoter in wild-type cells exposed to 0.2 M LiCl for 1 hr.…”

Section: Ypi1 Controls Intracellular Lithium Accumulation Through Botmentioning

confidence: 97%

“…For instance, efflux of cations through the ENA1 ATPase is mainly regulated at the transcriptional level, involving several signaling pathways that allow fine-tuned control of its expression. These pathways include diverse kinases (Hog1, PKA, or Snf1), and phosphatases, such as calcineurin, Ptc1, or Ppz1/Hal3 (Ruiz and Arino 2007). In contrast, Nha1 levels do not significantly vary, although Nha1's regulation also entails protein kinase and phosphatase activities in response to cation stress (Proft and Struhl 2004;Manlandro et al 2005;Kinclova-Zimmermannova and Sychrova 2006).…”

mentioning

confidence: 96%

“…Usually the ENA genes appear in clusters and different strains can have a different number of genes (from 1 to 5). ENA genes are normally expressed at very low levels but at least one member of the cluster (ENA1) is rapidly activated by salt stress or alkalinization of the medium (see Ruiz and Arino 2007; Rodriguez-Navarro and Benito 2010 for reviews). Besides sodium, the Ena1 ATPase can also transport potassium and lithium cations.…”

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Modulation of Yeast Alkaline Cation Tolerance by Ypi1 Requires Calcineurin

et al. 2012

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Ypi1 was discovered as an essential protein able to act as a regulatory subunit of the Saccharomyces cerevisiae type 1 protein phosphatase Glc7 and play a key role in mitosis. We show here that partial depletion of Ypi1 causes lithium sensitivity and that high levels of this protein confer a lithium-tolerant phenotype to yeast cells. Remarkably, this phenotype was independent of the role of Ypi1 as a Glc7 regulatory subunit. Lithium tolerance in cells overexpressing Ypi1 was caused by a combination of increased efflux of lithium, mediated by augmented expression of the alkaline cation ATPase ENA1, and decreased lithium influx through the Trk1,2 high-affinity potassium transporters. Deletion of CNB1, encoding the regulatory subunit of the calcineurin phosphatase, blocked Ypi1-induced expression of ENA1, normalized Li + fluxes, and abolished the Li + hypertolerant phenotype of Ypi1-overexpressing cells. These results point to a complex role of Ypi1 on the regulation of cation homeostasis, largely mediated by the calcineurin phosphatase.

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Rapid strain improvement through optimized evolution in the cytostat

Gilbert

Sangurdekar

Srienc

2009

Biotech & Bioengineering

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Acetate is present in lignocellulosic hydrolysates at growth inhibiting concentrations. Industrial processes based on such feedstock require strains that are tolerant of this and other inhibitors present. We investigated the effect of acetate on Saccharomyces cerevisiae and show that elevated acetate concentrations result in a decreased specific growth rate, an accumulation of cells in the G1 phase of the cell cycle, and an increased cell size. With the cytostat cultivation technology under previously derived optimal operating conditions, several acetate resistant mutants were enriched and isolated in the shortest possible time. In each case, the isolation time was less than 5 days. The independently isolated mutant strains have increased specific growth rates under conditions of high acetate concentrations, high ethanol concentrations, and high temperature. In the presence of high acetate concentrations, the isolated mutants produce ethanol at higher rates and titers than the parental strain and a commercial ethanol producing strain that has been analyzed for comparison. Whole genome microarray analysis revealed gene amplifications in each mutant. In one case, the LPP1 gene, coding for lipid phosphate phosphatase, was amplified. Two mutants contained amplified ENA1, ENA2, and ENA5 genes, which code for P-type ATPase sodium pumps. LPP1 was overexpressed on a plasmid, and the growth data at elevated acetate concentrations suggest that LPP1 likely contributes to the phenotype of acetate tolerance. A diploid cross of the two mutants with the amplified ENA genes grew faster than either individual haploid parent strain when 20 g/L acetate was supplemented to the medium, which suggests that these genes contribute to acetate tolerance in a gene dosage dependent manner.

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Function and Regulation of the Saccharomyces cerevisiae ENA Sodium ATPase System

Cited by 103 publications

References 102 publications

Nsf1/Ypl230w participates in transcriptional activation during non-fermentative growth and in response to salt stress in Saccharomyces cerevisiae

Nsf1/Ypl230w participates in transcriptional activation during non-fermentative growth and in response to salt stress in Saccharomyces cerevisiae

Modulation of Yeast Alkaline Cation Tolerance by Ypi1 Requires Calcineurin

Rapid strain improvement through optimized evolution in the cytostat

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