Function and repair of dental enamel – Potential role of epithelial transport processes of ameloblasts

Varga, Gábor; Kerémi, Beáta; Bori, Erzsébet; Földes, Anna

doi:10.1016/j.pan.2015.01.012

Cited by 13 publications

(24 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To test this possibility, we investigated whether fluoride exposure modulates transcellular HCO − 3 secretion in HAT-7 cells. Our data clearly showed that acute exposure to a wide range of fluoride concentrations caused no change in the rate of acidification of the cells when basolateral HCO − 3 uptake was blocked (Racz et al, 2017), indicating that fluoride has no direct acute inhibitory effect on HCO − 3 secretion, which we consider to be a crucial requirement for mineralization (Varga et al, 2015). Interestingly, we found that instead tight-junction formation was reduced in the presence of 1 mM fluoride, a concentration that resulted in no, or very little, change in HAT-7 cell viability.…”

Section: Significance Of and Potential Applications Of Gained Knowlmentioning

confidence: 60%

“…Bicarbonate ions secreted by ameloblasts neutralize protons that are produced during mineralization of the enamel suggesting a key role of bicarbonate as a buffer in tooth development (Bori et al, 2016;Josephsen et al, 2010;Lacruz, Nanci, Kurtz, et al, 2010;Lyaruu et al, 2008;Smith, 1998;Varga et al, 2015). In mouse incisors, maturationstage enamel stained with pH indicators shows acidic wide bands and neutral narrow stripes (Damkier et al, 2014;Josephsen et al, 2010).…”

Section: Present Knowledge On the Role Of Bicarbonate In Controllinmentioning

confidence: 99%

“…is not yet determined (Robinson, 2014;Varga, Kerémi, Bori, & Földes, 2015). In the secretory phase, the whole thickness of the enamel is formed, but its mineralization is only approximately 20%-30%.…”

Section: Introductionmentioning

confidence: 99%

“…Histochemical evidence has been presented for the expression of specific ion transporters in the ameloblast membrane (Bronckers, ; Josephsen et al., ), but their exact role in crystal growth is not yet determined (Robinson, ; Varga, Kerémi, Bori, & Földes, ). In the secretory phase, the whole thickness of the enamel is formed, but its mineralization is only approximately 20%–30%.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Importance of bicarbonate transport in pH control during amelogenesis — need for functional studies

et al. 2017

View full text Add to dashboard Cite

Dental enamel, the hardest mammalian tissue, is produced by ameloblasts. Ameloblasts show many similarities to other transporting epithelia although their secretory product, the enamel matrix, is quite different. Ameloblasts direct the formation of hydroxyapatite crystals, which liberate large quantities of protons that then need to be buffered to allow mineralization to proceed. Buffering requires a tight pH regulation and secretion of bicarbonate by ameloblasts. Many investigations have used immunohistochemical and knockout studies to determine the effects of these genes on enamel formation, but up till recently very little functional data were available for mineral ion transport. To address this, we developed a novel 2D in vitro model using HAT-7 ameloblast cells. HAT-7 cells can be polarized and develop functional tight junctions. Furthermore, they are able to accumulate bicarbonate ions from the basolateral to the apical fluid spaces. We propose that in the future, the HAT-7 2D system along with similar cellular models will be useful to functionally model ion transport processes during amelogenesis. Additionally, we also suggest that similar approaches will allow a better understanding of the regulation of the cycling process in maturation-stage ameloblasts, and the pH sensory mechanisms, which are required to develop sound, healthy enamel. K E Y W O R D Sameloblast, amelogenesis, bicarbonate, enamel, functional model, HAT-7, in vitro, ion transport, microfluorometry, pH regulation, transepithelial resistance, transwell

show abstract

Section: Significance Of and Potential Applications Of Gained Knowlmentioning

confidence: 60%

Section: Present Knowledge On the Role Of Bicarbonate In Controllinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Importance of bicarbonate transport in pH control during amelogenesis — need for functional studies

et al. 2017

View full text Add to dashboard Cite

show abstract

“…After that, the replacement of the degradated enamel matrix protein by tissue fl uid occurs at the transition stage. At the maturation stage, residual organic matrix is replaced by tissue fl uid which is replaced by mineral uptake associated with hydroxyapatite crystal formation and their growth in width and thickness 7,8) . During enamel formation, enzymes such as amelogenin, enamelin, ameloblastin and tuftalin control processes of mineral ion binding, crystal growth, formation of prismatic structure, cell signaling, secretion and protection of the mineral phase 9,10) .…”

Section: Introductionmentioning

confidence: 99%

Interrelationships between Bone Metabolism Markers and Deciduous Teeth and Mandible Properties in Polish Merino Sheep

Szabelska

Tatara

Krupski

2017

J. Hard Tissue Biology.

View full text Add to dashboard Cite

Considering existing similarities of bones and teeth formation and following mineralization, the aim of the study was to evaluate biochemical bone metabolism markers in growing Polish Merino sheep and their relationships with morphometric, densitometric and mechanical properties of deciduous mandibular teeth and mandible. Blood samples were collected from 28-day and 150-day-old sheep to determine serum concentrations of insulin-like growth factor I (IGF-I), bone-specifi c alkaline phosphatase (BAP), osteocalcin, C-terminal propeptide of type I procollagen (CICP), C-terminal telopeptides of type I collagen (CTX-I) and C-terminal telopeptides of type II collagen (CTX-II). Bone and tooth samples were collected from 150-day-old sheep and were evaluated morphologically, densitometrically and mechanically. Serum concentrations of BAP, OC and CTX-II were significantly lower in 150-day-old sheep when compared to the measurements performed 4 months earlier (P < 0.01). Negative correlation between CTX-I and OC concentrations in 150-day-old sheep was stated (P < 0.05). Majority of the significant correlations between tooth properties and serum biochemical bone metabolism indices were found in 28-day-old sheep indicating more intensive odontogenesis process of deciduous teeth than 4 months later. Positive correlations between morphological and densitometric properties of mandible and serum CICP dominated in 28-day-old sheep, while 4 months later CTX-II was the only one marker positively correlated with morphological, densitometric and mechanical properties of mandible (P < 0.05). Evaluation of bone and cartilage metabolism markers in serum may be utilized in further studies on regulation of dental and skeletal metabolism with environmental, physiological, pharmacological, nutritional and toxicological factors infl uencing mineralized tissue metabolism.

show abstract

KDF1 promotes ameloblast differentiation by inhibiting the IKK/IκB/NF‐κB axis

Liu,

Yu,

Sun

et al. 2024

Journal Cellular Physiology

View full text Add to dashboard Cite

Enamel protects teeth from external irritation and its formation involves sequential differentiation of ameloblasts, a dental epithelial cell. Keratinocyte differentiation factor 1 (KDF1) is important in the development of epithelial tissues and organs. However, the specific role of KDF1 in enamel formation and corresponding regulatory mechanisms are unclear. This study demonstrated that KDF1 was persistently expressed in all stages of ameloblast differentiation, through RNAscope in situ hybridization. KDF1 expression in the mouse ameloblast cell line LS8 was demonstrated via immunofluorescence assay. KDF1 was knocked out in LS8 cells using the CRISPR/Cas‐9 system or overexpressed in LS8 cells through lentiviral infection. In vitro ameloblast differentiation induction, quantitative reverse transcription PCR, western blot analysis, and alkaline phosphatase (ALP) assay indicated that knockout or overexpression of KDF1 in LS8 cells decreased or increased the mRNA and protein levels of several key amelogenesis markers, as well as ALP activity. Furthermore, liquid chromatography‐mass spectrometry and co‐immunoprecipitation analyses revealed that KDF1 can interact with the IKK complex, thereby inhibiting the NF‐κB pathway. Suppressing NF‐κB activity partially recovered the decreased ameloblast differentiation in LS8 cells induced by KDF1‐knockout. This study demonstrated that KDF1 can promote ameloblast differentiation of LS8 cells by inhibiting the IKK/IκB/NF‐κB axis, and is a potential target for functional enamel regeneration.

show abstract

Function and repair of dental enamel – Potential role of epithelial transport processes of ameloblasts

Cited by 13 publications

References 58 publications

Importance of bicarbonate transport in pH control during amelogenesis — need for functional studies

Importance of bicarbonate transport in pH control during amelogenesis — need for functional studies

Interrelationships between Bone Metabolism Markers and Deciduous Teeth and Mandible Properties in Polish Merino Sheep

KDF1 promotes ameloblast differentiation by inhibiting the IKK/IκB/NF‐κB axis

Contact Info

Product

Resources

About