Function and safety evaluation of Staphylococcus epidermidis with high esterase activity isolated from strong flavor Daqu

Inoculation of Zaopei with biofortification: Inducing variation in community structure and improving flavor compounds in Nongxiangxing baijiu

“…As a characteristic flavor substance, ethyl hexanoate (apple‐like flavor) is the main factor that inhibits the yield of excellent‐grade of N. baijiu . In the brewing process of baijiu , ethyl hexanoate is produced mainly by the pathway in which esterase (an extracellular enzyme) catalyzes hexanoic acid and ethanol as substrates (Xu et al., 2023). This combination might occur mainly in B. subtilis and S. epidermidis which have high esterifying power under facultative anaerobic and anaerobic conditions (Xu et al., 2022).…”

“…As a member of the high‐temperature‐resistant genus, Millerozyma was observed in Jiangxiangxing Daqu (Du et al., 2022), soy sauce (Wei et al., 2013), and soybean paste (Mallet et al., 2012), but there are few studies on the biofortification of Millerozyma farinosa using high esterase‐producing enzymes. The functional strain named Staphylococcus epidermidis YTPW‐4 exhibited high esterase activity, good environmental tolerance, and good safety characteristics (Xu, Yang et al., 2023). We hypothesized that: (1) biofortified inoculation led to the recombination of microbial communities in the ZP.…”

“…(2) Introducing specific strains of microorganisms into the fermentation process has the potential to enhance the levels of ethyl hexanoate and other aromatic compounds in baijiu . In our previous research, our team has documented the utilization of three highly esterifying strains ( B. subtilis , S. epidermidis , and M. farinosa ) in the production of bioenhanced Daqu (Xu, Wu et al., 2023). This provides valuable data support for us to improve the content of ethyl hexanoate in the baijiu by biofortified fermentation.…”

Assessment of the antibacterial effect of Barium Titanate nanoparticles against Staphylococcus epidermidis adhesion after addition to maxillofacial silicone

“…This test was performed to evaluate the antibacterial activity of BaTiO 3 against S. epidermidis , depending on optical density (OD) measurement using a spectrophotometer (APEL PD-303, Japan) set at 600 nm. 18 Brain heart infusion broth was used to grow and create the bacterial suspension. It was prepared according to the manufacturer’s instructions by suspending 34.5 grams of powder in one liter of distilled water and dissolving it completely, then autoclaved at 15 lbs.…”

Assessment of the antibacterial effect of Barium Titanate nanoparticles against Staphylococcus epidermidis adhesion after addition to maxillofacial silicone

“…This test was performed to evaluate the antibacterial activity of BaTiO 3 against S. epidermidis, depending on optical density (OD) measurement using a spectrophotometer (APEL PD-303, Japan) set at 600 nm. 16 Brain heart infusion broth was used to grow and create the bacterial suspension. It was prepared according to the manufacturer's instructions by suspending 34.5 grams of powder in one liter of distilled water and dissolving it completely, then autoclaved at 15 lbs.…”