Function and subcellular localization of Gcn5, a histone acetyltransferase in Candida albicans

Chang, Peng; Fan, Xueyi; Chen, Jiangye

doi:10.1016/j.fgb.2015.01.011

Cited by 67 publications

(76 citation statements)

References 60 publications

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“…This virus-specific requirement of AGL2 for the dcl2 up-regulation adds complexity to the model. invasive hyphal growth, induction of a thick stubby pseudohyphaelike form, and loss of pathogenesis (43). It is conceivable that a portion of genes regulated by SAGA includes essential genes for normal growth.…”

Section: Saga Mediates Virus-responsive Induction Of Rnai Key Genesmentioning

confidence: 99%

SAGA complex mediates the transcriptional up-regulation of antiviral RNA silencing

Andika

Jamal

Kondō

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Pathogen recognition and transcriptional activation of defenserelated genes are crucial steps in cellular defense responses. RNA silencing (RNAi) functions as an antiviral defense in eukaryotic organisms. Several RNAi-related genes are known to be transcriptionally up-regulated upon virus infection in some host organisms, but little is known about their induction mechanism. A phytopathogenic ascomycete, Cryphonectria parasitica (chestnut blight fungus), provides a particularly advantageous system to study RNAi activation, because its infection by certain RNA viruses induces the transcription of dicer-like 2 (dcl2) and argonaute-like 2 (agl2), two major RNAi players. To identify cellular factors governing activation of antiviral RNAi in C. parasitica, we developed a screening protocol entailing multiple transformations of the fungus with cDNA of a hypovirus mutant lacking the RNAi suppressor (CHV1-Δp69), a reporter construct with a GFP gene driven by the dcl2 promoter, and a random mutagenic construct. Screening for GFP-negative colonies allowed the identification of sgf73, a component of the SAGA (Spt-Ada-Gcn5 acetyltransferase) complex, a well-known transcriptional coactivator. Knockout of other SAGA components showed that the histone acetyltransferase module regulates transcriptional induction of dcl2 and agl2, whereas histone deubiquitinase mediates regulation of agl2 but not dcl2. Interestingly, full-scale induction of agl2 and dcl2 by CHV1-Δp69 required both DCL2 and AGL2, whereas that by another RNA virus, mycoreovirus 1, required only DCL2, uncovering additional roles for DCL2 and AGL2 in viral recognition and/or RNAi activation. Overall, these results provide insight into the mechanism of RNAi activation.Cryphonectria parasitica | hypovirus | RNAi | mycovirus | antiviral defense

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Section: Saga Mediates Virus-responsive Induction Of Rnai Key Genesmentioning

confidence: 99%

SAGA complex mediates the transcriptional up-regulation of antiviral RNA silencing

Andika

Jamal

Kondō

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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show abstract

“…To express the wild-type MRR2 from the ADH1 promoter, the full-length of MRR2 was amplified with the primers MRR2-F/R and was inserted into pCPC20 (the general structure of pCPC20 is shown in Supplementary Fig. S1) [16] using ClonExpress TM II One-step Cloning Kit (Vazyme Biotech, Nanjing, China) to obtain pWY21. Primers CP19/28 were used to amplify (Table 4) were also cloned into pCPC20 to obtain pWY22 for expression of the mutated MRR2 alleles.…”

Section: Plasmid Constructionmentioning

confidence: 99%

Mutations in transcription factor Mrr2p contribute to fluconazole resistance in clinical isolates of Candida albicans

Wang

Liu

Shi

et al. 2015

International Journal of Antimicrobial Agents

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“…Paralleling Mediator, the overall SAGA complexes of S. cerevisiae and C. albicans show excellent subunit sequence similarity ( Figure 3). Functional analysis of the catalytic subunit of the complex encoded by GCN5 showed its involvement in invasive and filamentous growth, in stress response, and ultimately in virulence [40]. Genomic analysis of the binding of the coactivator Ada2 to chromatin, and of the transcriptional consequences of loss of Ada2 itself, has established roles for SAGA in response to oxidative stress and drugs as well as in glycolysis [41].…”

Section: Sagamentioning

confidence: 99%

“…Common names in C. albicans have not been selected for the underlined S. cerevisiae genes. [38,40,68,69].…”

Section: Nua4mentioning

confidence: 99%

Metabolic regulation in model ascomycetes – adjusting similar genomes to different lifestyles

et al. 2015

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Function and subcellular localization of Gcn5, a histone acetyltransferase in Candida albicans

Cited by 67 publications

References 60 publications

SAGA complex mediates the transcriptional up-regulation of antiviral RNA silencing

SAGA complex mediates the transcriptional up-regulation of antiviral RNA silencing

Mutations in transcription factor Mrr2p contribute to fluconazole resistance in clinical isolates of Candida albicans

Metabolic regulation in model ascomycetes – adjusting similar genomes to different lifestyles

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