Function of Lipid Storage Droplet 1 (Lsd1) in Wing Development of Drosophila melanogaster

Men, Tran Thanh; Binh, Tran Duy; Yamaguchi, Masamitsu; Huy, Nguyen Tien; Kamei, Kaeko

doi:10.3390/ijms17050648

Cited by 18 publications

(12 citation statements)

References 38 publications

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“…In starvation conditions, acute TG lipolysis is induced in Drosophila , which is attenuated in the FB cells of AKHR mutant flies. Also, in vitro and in vivo studies have identified that Lsd-1 (PLIN1) is a PKA phosphorylation target that has a central role as a pro-lipolytic effector of the AKH/AKHR pathway on lipid droplet surface 73 . Our results show that Dome loss in the FB significantly reduced the effect of a HSD on AKHR and FASN expression, which may explain the decrease in TGs storage and a low expression of lsd-1 , despite the energy oversupply of a HSD (Fig.…”

Section: Discussionmentioning

confidence: 99%

Domeless receptor loss in fat body tissue reverts insulin resistance induced by a high-sugar diet in Drosophila melanogaster

Lourido

Quenti

Salgado-Canales

et al. 2021

Sci Rep

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Insulin resistance is a hallmark of type 2 diabetes resulting from the confluence of several factors, including genetic susceptibility, inflammation, and diet. Under this pathophysiological condition, the dysfunction of the adipose tissue triggered by the excess caloric supply promotes the loss of sensitivity to insulin at the local and peripheral level, a process in which different signaling pathways are involved that are part of the metabolic response to the diet. Besides, the dysregulation of insulin signaling is strongly associated with inflammatory processes in which the JAK/STAT pathway plays a central role. To better understand the role of JAK/STAT signaling in the development of insulin resistance, we used a simple organism, Drosophila melanogaster, as a type 2 diabetes model generated by the consumption of a high-sugar diet. In this model, we studied the effects of inhibiting the expression of the JAK/STAT pathway receptor Domeless, in fat body, on adipose metabolism and glycemic control. Our results show that the Domeless receptor loss in fat body cells reverses both hyperglycemia and the increase in the expression of the insulin resistance marker Nlaz, observed in larvae fed a high sugar diet. This effect is consistent with a significant reduction in Dilp2 mRNA expression and an increase in body weight compared to wild-type flies fed high sugar diets. Additionally, the loss of Domeless reduced the accumulation of triglycerides in the fat body cells of larvae fed HSD and also significantly increased the lifespan of adult flies. Taken together, our results show that the loss of Domeless in the fat body reverses at least in part the dysmetabolism induced by a high sugar diet in a Drosophila type 2 diabetes model.

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Section: Discussionmentioning

confidence: 99%

Domeless receptor loss in fat body tissue reverts insulin resistance induced by a high-sugar diet in Drosophila melanogaster

Lourido

Quenti

Salgado-Canales

et al. 2021

Sci Rep

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“…Later in Drosophila development, wing imaginal discs accumulate surprisingly high levels of LDs that depend upon perilipin expression, which in turn is induced by a developmental transcription factor, vestigial ( Fauny et al, 2005 ). If perilipin is knocked down, mitochondrial-derived reactive oxygen species accumulate in the wing disc, many cells undergo apoptosis, and the wing remains small and misshapen ( Men et al, 2016 ). As a final example, Drosophila neuronal stem cells are maintained in a niche where the surrounding glia cells contain ample LDs to protect lipids from peroxidation reactions ( Bailey et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Fat-containing cells are eliminated duringDictyosteliumdevelopment

Kornke

Maniak

2017

Biology Open

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Triacylglycerol is a universal storage molecule for metabolic energy in living organisms. However, Dictyostelium amoebae, that have accumulated storage fat from added fatty acids do not progress through the starvation period preceding the development of the durable spore. Mutants deficient in genes of fat metabolism, such as fcsA, encoding a fatty acid activating enzyme, or dgat1 and dgat2, specifying proteins that synthesize triacylglycerol, strongly increase their chances to contribute to the spore fraction of the developing fruiting body, but lose the ability to produce storage fat efficiently. Dictyostelium seipin, an orthologue of a human protein that in patients causes the complete loss of adipose tissue when mutated, does not quantitatively affect fat storage in the amoeba. Dictyostelium seiP knockout mutants have lipid droplets that are enlarged in size but reduced in number. These mutants are as vulnerable as the wild type when exposed to fatty acids during their vegetative growth phase, and do not efficiently enter the spore head in Dictyostelium development.

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“…The wandering 3rd-instar larvae were dissected in PBS to collect wing imaginal discs. Approximately 20 to 30 wing imaginal discs were fixed in 4% formaldehyde in PBS and reacted with antibodies as described previously [67]. Anti-dLipin antibody [20], provided kindly by Prof. Dr. Michael Lehmann (University of Arkansas, Arkansas, USA), was used at a 1:3000 dilution, and then anti-rabbit IgG Alexa Fluor TM 594 (Molecular Probes, Invitrogen, Carlsbad, CA, USA) was used at a dilution of 1:800.…”

Section: Methodsmentioning

confidence: 99%

The Function of Lipin in the Wing Development of Drosophila melanogaster

Binh

Pham

Nishihara

et al. 2019

IJMS

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Lipin is evolutionarily conserved from yeast to mammals. Although its roles in lipid metabolism in adipocyte tissue, skeletal muscle, and the liver, and as a transcriptional co-activator are known, its functions during development are still under investigation. In this study, we analyzed the role of Drosophila lipin (dLipin) in development. Specifically, we showed that the tissue-selective knockdown of dLipin in the wing pouch led to an atrophied wing. Elevated DNA damage was observed in the wing imaginal disc of dLipin-knockdown flies. dLipin dysfunction induced accumulation of cells in S phase and significantly reduced the number of mitotic cells, indicating DNA damage-induced activation of the G2/M checkpoint. Reduced expression of cyclin B, which is critical for the G2 to M transition, was observed in the margin of the wing imaginal disc of dLipin-knockdown flies. The knockdown of dLipin led to increased apoptotic cell death in the wing imaginal disc. Thus, our results suggest that dLipin is involved in DNA replication during normal cell cycle progression in wing development of Drosophila melanogaster.

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Function of Lipid Storage Droplet 1 (Lsd1) in Wing Development of Drosophila melanogaster

Cited by 18 publications

References 38 publications

Domeless receptor loss in fat body tissue reverts insulin resistance induced by a high-sugar diet in Drosophila melanogaster

Domeless receptor loss in fat body tissue reverts insulin resistance induced by a high-sugar diet in Drosophila melanogaster

Fat-containing cells are eliminated duringDictyosteliumdevelopment

The Function of Lipin in the Wing Development of Drosophila melanogaster

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