Function of the mammalian oviductal sperm reservoir

Töpfer‐Petersen, Edda; Wagner, Andrea; Friedrich, Julia; Petrunkina, A. M.; Ekhlasi-Hundrieser, Mahnaz; Waberski, Dagmar; Drommer, W.

doi:10.1002/jez.1157

Cited by 96 publications

(67 citation statements)

References 24 publications

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“…Indeed, in species with a long interval between the onset of oestrus and ovulation, mechanisms to suppress completion of capacitation need to assume dominance over those that would promote achievement of full capacitation (Hunter, 1996(Hunter, , 1997. These interpretations have now been largely accepted (e.g., Smith, 1998;Rodriguez-Martinez et al, 2001Töpfer-Petersen et al, 2002;Tienthai et al, 2003Tienthai et al, , 2004.…”

Section: An Evolutionary Perspectivementioning

confidence: 99%

“…For example, bovine spermatozoa bind to fucose-containing molecules on the surface of the caudal isthmus Suarez, 1998Suarez, , 2001Revah et al, 2000) whereas complex mannose structures appear to be involved in the initial sperm binding mechanism in pig oviducts . Recent reviews on the functional sperm reservoir have been given by Suarez (2001), Töpfer-Petersen et al (2002) and Rodriguez-Martinez et al (2005).…”

Section: Functional Sperm Reservoirmentioning

confidence: 99%

“…An interpretation that remains valid at the time of writing this essay is that peri-ovulatory activation of bound spermatozoa is prompted in part by an influx of Ca 2þ ions from the endosalpinx (see Petrunkina et al, 2001;Töpfer-Petersen et al, 2002). Specific ion channels would clearly be required and these have yet to be Molecular Reproduction and Development.…”

Section: Catecholamine and Ca 2þ Ionmentioning

confidence: 99%

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Sperm release from oviduct epithelial binding is controlled hormonally by peri‐ovulatory graafian follicles

Hunter

2007

Molecular Reproduction Devel

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To avoid inappropriate conclusions being drawn from the extensive use of in vitro preparations of sperm-oviduct epithelial binding, it is recalled that events in the genital tract of mammals are regulated by the gonads, primarily by their changing secretion of steroid hormones. Key observations from in vivo models are used to emphasise the dynamic interactions between viable sperm cells and the caudal (distal) portion of the oviduct isthmus, the site of the functional sperm reservoir. These include (1) preovulatory arrest and epithelial binding of intact sperm cells and thereby suppression of completion of capacitation, (2) peri-ovulatory activation and release from binding of discrete sub-populations of competent spermatozoa, and (3) post-ovulatory liberation of large numbers of spermatozoa. These observations underline the influence of endocrine regulation of sperm binding and release by peri-ovulatory Graafian follicles, a point brought out by the enhanced sperm release prompted by diverse treatments with solutions of progesterone. In the light of this evidence, the suitability of in vitro preparations for clarifying physiological events should be questioned, especially if myosalpingeal catecholamines diffusing out of the autonomic nervous system contribute to sperm activation and/or release. None of this is to infer that sperm cells themselves are without influence on their epithelial binding reaction(s). Nor is it to suggest that in vitro models of sperm-oviduct binding are without relevance to the development of sperm evaluation technologies. However, pre-ovulatory sperm-epithelial binding and a regulated peri-ovulatory release should be seen as vital tactics in the overall strategy of achieving successful monospermic fertilisation. Mol. Reprod. Dev. 75: 167-174, 2008.

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Section: An Evolutionary Perspectivementioning

confidence: 99%

Section: Functional Sperm Reservoirmentioning

confidence: 99%

Section: Catecholamine and Ca 2þ Ionmentioning

confidence: 99%

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Sperm release from oviduct epithelial binding is controlled hormonally by peri‐ovulatory graafian follicles

Hunter

2007

Molecular Reproduction Devel

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“…Instead, a second population reaches the oviduct several hours after insemination and most are trapped in the isthmus and are held until ovulation is eminent. During this time, spermatozoa bind to ciliated epithelial cells of the isthmus forming what is called the sperm reservoir or oviductal reservoir [8,9]. In the pig, at least 4,000 -5,000 spermatozoa are present in the isthmus before ovulation occurs [10].…”

Section: Introductionmentioning

confidence: 99%

Oviduct binding ability of porcine spermatozoa develops in the epididymis and can be advanced by incubation with caudal fluid

et al. 2015

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Abstract.The sperm reservoir is formed when spermatozoa bind to the epithelium of the utero-tubal junction and caudal isthmus of the oviduct. It is an important mechanism that helps synchronize the meeting of gametes by regulating untimely capacitation and polyspermic fertilisation. This study investigated the influence of epididymal maturation and caudal fluid on the ability of spermatozoa to bind to oviduct epithelium using a model porcine oviduct explant assay. Spermatozoa from the rete testis, middle caput (E2-E3), middle corpus (E6) and cauda (E8) of Large White or Large White x Landrace boars at 10-14 months of age were diluted in modified Androhep solution and incubated with porcine oviduct explants. Results reported in this study support our hypothesis that testicular spermatozoa need to pass through the regions of the epididymis in order to acquire the ability to bind to the oviduct. There was a sequential increase in the number of spermatozoa that bound to oviduct explants from the rete testis to caudal epididymis. Binding of caudal spermatozoa to isthmic explants was the highest (15.0 ± 1.2 spermatozoa per 1.25 mm 2 ; mean ± standard error of the mean; P ≤ 0.05) and lowest by spermatozoa from the rete testis (2.0 ± 0.3 per 1.25 mm 2 ), and higher to isthmus from sows compared to gilts (35.8 ± 6.7 per 1.25 mm 2 vs. 14.8 ± 3.0 per 1.25 mm 2 ; P ≤ 0.05). Binding of ejaculated spermatozoa to porcine isthmus was higher than for caudal spermatozoa (26.3 ± 1.4 per 1.25 mm 2 vs. 15.0 ± 0.8 per 1.25 mm 2 ; P ≤ 0.05), and higher to porcine than to bovine isthmus (26.3 ± 2.3 per 1.25 mm 2 vs. 18.8 ± 1.9 per 1.25 mm 2 ; P ≤ 0.05). Incubation of spermatozoa from the caput and corpus in caudal fluid 2 increased the ability of spermatozoa to bind to oviduct epithelium (P ≤ 0.05). In conclusion, the capacity of testicular spermatozoa to bind to oviduct epithelium increases during their maturation in the epididymis, and can be advanced by components of the caudal fluid.

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“…BSP proteins play a crucial role in the formation of the oviductal sperm reservoir (Gwathmey et al 2006), which serves to store sperm and reduce the chances of polyspermic fertilization (Hunter 1973). This is of paramount importance: for fertilization to be successful, functional, competent gametes must meet at the site of fertilization at the right time (Töpfer-Petersen et al 2002). Sperm coated with excess amounts of one or more of the three BSP proteins might not be released from the reservoir in a timely manner.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation increases coating of bull sperm by seminal plasma binder of sperm proteins BSP1, BSP3, and BSP5

Ardón

Suárez

2013

Reproduction

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Artificial insemination with frozen semen allows affordable, worldwide dissemination of gametes with superior genetics. Nevertheless, sperm are damaged by the cryopreservation process. Elucidating the molecular effects of cryopreservation on sperm could suggest methods for improving fertility of frozen/thawed semen. This study was undertaken to examine the effect of cryopreservation on the coating of sperm by binder of sperm (BSP) proteins in seminal plasma. BSP proteins are secreted by the seminal vesicles and coat the surface of sperm by partially intercalating into the outer leaflet of the sperm plasma membrane. The BSP proteins are known to play roles in the formation of the oviductal sperm storage reservoir and in sperm capacitation. We investigated the effects of cryopreservation on the sperm BSP protein coat using Bovipure to separate live sperm from extended semen and then assaying the amounts of BSP proteins on sperm using quantitative western blotting with custom-made antibodies against unique sequences of each BSP protein. Greater amounts of all three BSP proteins (BSP1, BSP3, and BSP5) were detected on frozen/thawed sperm than on fresh sperm. Furthermore, the reduction of BSP3 from 15 to 13 kDa in mass, which occurs during incubation of sperm under mild capacitating conditions, was enhanced by cryopreservation. We concluded that freezing alters the BSP protein coating on sperm, which could account in part for reduced fertility of cryopreserved semen samples.

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Function of the mammalian oviductal sperm reservoir

Cited by 96 publications

References 24 publications

Sperm release from oviduct epithelial binding is controlled hormonally by peri‐ovulatory graafian follicles

Sperm release from oviduct epithelial binding is controlled hormonally by peri‐ovulatory graafian follicles

Oviduct binding ability of porcine spermatozoa develops in the epididymis and can be advanced by incubation with caudal fluid

Cryopreservation increases coating of bull sperm by seminal plasma binder of sperm proteins BSP1, BSP3, and BSP5

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