Function of the pentose phosphate pathway and its key enzyme, transketolase, in the regulation of the meiotic cell cycle in oocytes

Kim, Yunna; Kim, Eun Young; Seo, You‐Mi; Yoon, Tae Ki; Lee, Woo-Sik; Lee, Kyung‐Ah

doi:10.5653/cerm.2012.39.2.58

Cited by 33 publications

(33 citation statements)

References 26 publications

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“…6A-C). Because pentose phosphate pathway (PPP) was known to control maturation and development [30], expression level of PPP-related factors, such as Gpi1 , G6pd2 , Rpe , Rpia , Tkt , Taldo1 , Rbks , and Prps1 . The expression levels of the majority of PPP-related genes were reduced in Prkar2b -deficient oocytes, especially in Rpe and Tkt cases (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…In most organisms, the PPP is essential for the synthesis of nicotinamide adenine dinucleotide phosphate (NADPH), as well as five-carbon sugars [39], nucleotides, fatty acids, and neurotransmitters [30]. Recently, several studies reported that the PPP is involved in the metabolic pathways of various cells, including cancer cells and oocytes [30, 40].…”

Section: Discussionmentioning

confidence: 99%

“…Recently, several studies reported that the PPP is involved in the metabolic pathways of various cells, including cancer cells and oocytes [30, 40]. TKT, an enzyme in the PPP, plays a critical role in energy production and nucleic acid synthesis during embryonic development [41].…”

Section: Discussionmentioning

confidence: 99%

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Knockdown of PRKAR2B Results in the Failure of Oocyte Maturation

Yoon¹,

Jang²,

Kim³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Cyclic adenosine monophosphate (cAMP)-dependent type 2 regulatory subunit beta (Prkar2b) is a regulatory isoform of cAMP-dependent protein kinase (PKA), which is the primary target for cAMP actions. In oocytes, PKA and the pentose phosphate pathway (PPP) have important roles during the germinal vesicle (GV) stage arrest of development. Although the roles of the PKA signal pathway have been studied in the development of oocyte, there has been no report on the function of PRKAR2B, a key regulator of PKA. Methods: Using reverse transcription polymerase chain reaction (RT-PCR), quantitative real-time PCR (qRT-PCR), immunohistochemistry, and immunofluorescence, we determined the relative expression of Prkar2b in various tissues, including ovarian follicles, during oocyte maturation. Prkar2b-interfering RNA (RNAi) microinjection was conducted to confirm the effect of Prkar2b knockdown, and immunofluorescence, qRT-PCR, and time-lapse video microscopy were used to analyze Prkar2b-deficient oocytes. Results: Prkar2b is strongly expressed in the ovarian tissues, particularly in the growing follicle. During oocyte maturation, the highest expression of Prkar2b was during metaphase I (MI), with a significant decrease at metaphase II (MII). RNAi-mediated Prkar2b suppression resulted in MI-stage arrest during oocyte development, and these oocytes exhibited abnormal spindle formation and chromosome aggregation. Expression of other members of the PKA family (except for Prkaca) were decreased, and the majority of the PPP factors were also reduced in Prkar2b-deficient oocytes. Conclusion: These results suggest that Prkar2b is closely involved in the maturation of oocytes by controlling spindle formation and PPP-mediated metabolism.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Knockdown of PRKAR2B Results in the Failure of Oocyte Maturation

Yoon¹,

Jang²,

Kim³

et al. 2018

Cell Physiol Biochem

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“…Proteins encoded by the SSBP2 and TKT genes are involved in cellular growth, proliferation and metabolism and are thus capable of altering developmental trajectories. For example, TKT is involved in carbohydrate metabolism39 and could contribute to the later-in-life increased adiposity and risk of metabolic syndrome in children and adults born post-term 6 7…”

Section: Discussionmentioning

confidence: 99%

“…TKT dysregulation has an important role in cellular growth rates, oocyte cell cycle progression and maturation 39. The TKT gene encodes a protein that contributes to the main carbohydrate metabolic pathways by connecting the PPP to glycolysis.…”

Section: Discussionmentioning

confidence: 99%

GWAS on prolonged gestation (post-term birth): analysis of successive Finnish birth cohorts

et al. 2017

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Abstract:Background Gestation is a crucial timepoint in human development. Deviation from a term gestational age correlates with both acute and long-term adverse health effects for the child. Both being born pre and post-term, i.e. having short and long gestational ages, are heritable and influenced by the preand perinatal environment. Despite the obvious heritable component, specific genetic influences underlying differences in gestational age are poorly understood. MethodsWe investigated the genetic architecture of gestational age in 9,141 individuals, including 1,167 born post-term, across two Northern Finland cohorts (NNFBC) born in 1966 or 1986. ResultsHere we identify one globally significant intronic genetic variant within the ADAMTS13 gene that is associated with prolonged gestation (p=4.85x10 -8 ). Additional variants that reached suggestive levels of significance were identified within introns at the ARGHAP42 and TKT genes, and in the upstream (5') intergenic regions of the B3GALT5 and SSBP2 genes. The variants near the ADAMTS13, B3GALT5, SSBP2 and TKT loci are linked to alterations in gene expression levels (cis-eQTLs). Luciferase assays confirmed the allele specific enhancer activity for the BGALT5 and TKT loci. ConclusionsOur findings provide the first evidence of a specific genetic influence associated with prolonged gestation. This study forms a foundation for a better understanding of the genetic and long term health risks faced by induced and post-term individuals. The long-term risks for induced individuals who have a previously overlooked post-term potential may be a major issue for current health providers. including an increased need for intervention during labour and risk factors for truncal obesity, insulin resistance, altered lipids and elevated blood pressure [6,7]. Thus, there is a vital need to understand the role of genetic variants on post-term birth.The acute health risks, for both the mother and the child, of being born post-term are well and possibly face the long-term health risks of post-term birth without actually having been born post-term (due to pregnancy ending from obstetric management). Materials and Methods: SubjectsWe undertook a discovery-replication study of two successive birth cohorts from Northern 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 GHT, and preterm birth; the remaining represented a random sample of the cohort). Genotyping was completed using the OmniExpresse Exome Chip and the Beadstudio 3.1 algorithm. Defining the post-term dataset:The gestational ages of the individuals in the 1966 and 1986 NFBC cohorts were calculated at their first antenatal visit. For the NFBC1966 cohort gestational age was calculated through last menstrual period. In the NFBC1986 cohort gestational age was based on ultrasound at <20 weeks of gestation or on the last menstral period, with discrepant ca...

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The making of an embryo in a basal metazoan: Proteomic analysis in the sea anemone Nematostella vectensis

et al. 2015

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Cnidarians are widely distributed basal metazoans that play an important role in the marine ecosystem. Their genetic diversity and dispersal depends on successful oogenesis, fertilization and embryogenesis. To understand the processes that lead to successful embryogenesis in these basal organisms, we conducted comparative proteomics on the model sea anemone Nematostella vectensis. We examined four developmental stages from oocyte maturation through early embryogenesis, as well as the oocyte jelly sac in which fertilization and embryogenesis take place. Our analysis revealed 37 stage-specifically expressed proteins, including cell cycle, cellular energy related and DNA replication proteins and transcription regulators. Using in situ hybridization, we show that within the mesenteria, two cell types support successful oocyte development and embryogenesis. Large somatic supporting cells synthesize vitellogenin, the most abundant egg yolk protein within the oocyte, whereas mesenteria gland cells synthesize mucin 5B, which was found to be the main component of the jelly sac. These findings shed light on the sexual reproduction program in cnidarians and suggest a high conservation with proteins governing oogenesis in Bilateria.

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Function of the pentose phosphate pathway and its key enzyme, transketolase, in the regulation of the meiotic cell cycle in oocytes

Cited by 33 publications

References 26 publications

Knockdown of PRKAR2B Results in the Failure of Oocyte Maturation

Knockdown of PRKAR2B Results in the Failure of Oocyte Maturation

GWAS on prolonged gestation (post-term birth): analysis of successive Finnish birth cohorts

The making of an embryo in a basal metazoan: Proteomic analysis in the sea anemone Nematostella vectensis

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