Function of Transcription Factors PoMYB12, PoMYB15, and PoMYB20 in Heat Stress and Growth of Pleurotus ostreatus

Abstract

MYB transcription factors (TFs) have been extensively studied in plant abiotic stress responses and growth and development. However, the role of MYB TFs in the heat stress response and growth and development of Pleurotus ostreatus remains unclear. To investigate the function of PoMYB12, PoMYB15, and PoMYB20 TFs in P. ostreatus, mutant strains of PoMYB12, PoMYB15, and PoMYB20 were generated using RNA interference (RNAi) and overexpression (OE) techniques. The results indicated that the mycelia of OE-PoMYB12, OE-PoMYB20, and RNAi-PoMYB15 mutant strains exhibited positive effects under heat stress at 32 °C, 36 °C, and 40 °C. Compared to wild-type strains, the OE-PoMYB12, OE-PoMYB20, and RNAi-PoMYB15 mutant strains promoted the growth and development of P. ostreatus. These mutant strains also facilitated the recovery of growth and development of P. ostreatus after 24 h of 36 °C heat stress. In conclusion, the expression of PoMYB12 and PoMYB20 supports the mycelium’s response to heat stress and enhances the growth and development of P. ostreatus, whereas PoMYB15 produces the opposite effect.

Genome‐wide analysis of bZIP gene family members in Pleurotus ostreatus, and potential roles of PobZIP3 in development and the heat stress response

“…Increasingly, sequenced genomes facilitate the discovery of potential TFs that affect key agronomic traits. Some TFs of P. ostreatus were characterized and analysed, 20 PoMYB genes were identified and expression analysis of them showed that PoMYB12 , PoMYB15 and PoMYB20 showed extremely high expression levels after heat stress (Wang et al., 2018 ), then, the function of them was investigated that overexpression of PoMYB12 and PoMYB20 , along with RNAi of PoMYB15 , improved recovery growth post mycelial heat stress, and accelerated the growth and development of P. ostreatus (Yuan et al., 2023 ). And the Zn(II) 2 Cys 6 zinc cluster‐encoding gene family in P. ostreatus also been characterized and analysed, it showed that 13 PoZCP s may participate in the development of the heat stress response (Ding et al., 2022 ).…”