Function of von Willebrand factor in haemostasis and thrombosis

Reininger, Armin J.

doi:10.1111/j.1365-2516.2008.01848.x

Cited by 159 publications

(163 citation statements)

References 125 publications

(156 reference statements)

Supporting

Mentioning

155

Contrasting

Unclassified

Order By: Relevance

“…62 The influence of shear stress on the activity of platelets and vWF has been discussed in other review articles. 57,58,63,115 Others have linked the deterioration in cerebral microcirculation (cerebral hypoperfusion) to the onset of protein conformational disorders such as Alzheimer's disease. [116][117][118] It is suggested that reduced cerebral blood flow may replace old age as a risk factor for Alzheimer's disease.…”

Section: Implications In Physiology and Bioprocessingmentioning

confidence: 99%

The effects of shear flow on protein structure and function

Bekard

Asimakis

Bertolini³

et al. 2011

Biopolymers

180

140

View full text Add to dashboard Cite

Toxoplasma gondii usually causes an asymptomatic and then latent infection in human adults; however, a potentially fatal disseminated form can occur in immunocompromised patients. Given that the diagnosis of acute Toxoplasma infection, as opposed to latent disease, relies on finding direct evidence of T. gondii infection in tissue, pathologic examination is critical. There have only been a few reports describing the cytomorphology of Toxoplasma in exfoliative cytology, and no reports of the findings in Thin Prep. In this report, we describe a fatal case of toxoplasmosis in a cardiac transplant patient that was diagnosed by respiratory cytopathology. Although the extracellular organisms were well visualized on the Wright‐Giemsa stained cytospin, they were only faintly seen on the Pap‐stained cytospin trapped within mucin and were not easily appreciated on the ThinPrep slides nor the H&E stained cell block sections. An immunohistochemical stain for Toxoplasma performed on the cell block was strongly positive, and an autopsy performed on the patient confirmed disseminated infection. Our case illustrates that the diagnosis of Toxoplasma in exfoliative cytology specimens can be challenging since organisms are not well visualized on ThinPrep or Pap‐stained material; therefore, Wright‐Giemsa stained material can be particularly helpful. Diagn. Cytopathol. 2012. © 2011 Wiley Periodicals, Inc.

show abstract

Section: Implications In Physiology and Bioprocessingmentioning

confidence: 99%

The effects of shear flow on protein structure and function

Bekard

Asimakis

Bertolini³

et al. 2011

Biopolymers

180

140

View full text Add to dashboard Cite

show abstract

“…D-dimer is the FDP whose level is used as a clinical indicator of ongoing coagulation. D-dimer increases in thrombotic conditions such as disseminated intravascular coagulation, deep venous thrombosis, and pulmonary embolism (5,10,23). We analyzed the levels of vWF and D-dimer in mouse blood and the number of microthrombi in the liver.…”

Section: Role Of the Osmotically Regulated Transcription Factor Nfat5mentioning

confidence: 99%

“…For example, arterial thrombosis can be triggered by rupture of atherosclerotic plaques that leads to endothelial damage. However, venous thrombosis can also occur in the presence of an intact endothelium, making the triggering mechanism less apparent (1,4,5).…”

mentioning

confidence: 99%

“…This conformation occurs, for example, after secretion of vWF from endothelial cells before cleavage by ADAMTS13 protease, or under high shear stress in stenosed arteries. Numerous coagulation factors are then activated, resulting in growth of blood clots (4,5,(8)(9)(10). A number of population-based studies have suggested that elevated levels of vWF are a risk factor for thrombogenesis, especially in patients with previous cardiovascular diseases and older people (9).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Secretion of von Willebrand factor by endothelial cells links sodium to hypercoagulability and thrombosis

Dmitrieva

Burg

2014

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Hypercoagulability increases risk of thrombi that cause cardiovascular events. Here we identify plasma sodium concentration as a factor that modulates blood coagulability by affecting the production of von Willebrand factor (vWF), a key initiator of the clotting cascade. We find that elevation of salt over a range from the lower end of what is normal in blood to the level of severe hypernatremia reversibly increases vWF mRNA in endothelial cells in culture and the rate of vWF secretion from them. The high NaCl increases expression of tonicity-regulated transcription factor NFAT5 and its binding to promoter of vWF gene, suggesting involvement of hypertonic signaling in vWF up-regulation. To elevate NaCl in vivo, we modeled mild dehydration, subjecting mice to water restriction (WR) by feeding them with gel food containing 30% water. Such WR elevates blood sodium from 145.1 ± 0.5 to 150.2 ± 1.3 mmol/L and activates hypertonic signaling, evidenced from increased expression of NFAT5 in tissues. WR increases vWF mRNA in liver and lung and raises vWF protein in blood. Immunostaining of liver revealed increased production of vWF protein by endothelium and increased number of microthrombi inside capillaries. WR also increases blood level of D-dimer, indicative of ongoing coagulation and thrombolysis. Multivariate regression analysis of clinical data from the Atherosclerosis Risk in Communities Study demonstrated that serum sodium significantly contributes to prediction of plasma vWF and risk of stroke. The results indicate that elevation of extracellular sodium within the physiological range raises vWF sufficiently to increase coagulability and risk of thrombosis.blood clotting | HUVEC | osmotic stress

show abstract

“…The first -mediates the adhesion of platelets to the subendothelial matrix after vascular injury (16,17) and the second -protects the VIII factor from proteolytic degradation (18). vWF is released into circulation by secretion from endothelial cells (accumulates in Weibel-Palade bodies) and platelets (their alpha granules) (19,20).…”

Section: Introductionmentioning

confidence: 99%