Function of γ-Tubulin in Plants

Eckardt, Nancy A.

doi:10.1105/tpc.106.043976

Cited by 7 publications

(4 citation statements)

References 17 publications

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“…Plant g-tubulin does not heterodimerise with either of them. Instead, it plays a central role in the acentrosomal nucleation and organization of microtubule arrays in plants (Eckardt, 2006). In most plants, tubulins are encoded by multigene families.…”

Section: Introductionmentioning

confidence: 99%

Cloning and expression of the tubulin genes in barley

Radchuk

Sreenivasulu

Blume

et al. 2008

Cell Biology International

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Tubulins are encoded by small gene families in plants. Based on the barley EST collection, cDNAs for alpha-, beta-, and gamma-tubulins were selected. Five genes for alpha-tubulin, eight newly identified beta-tubulin sequences and one gamma-tubulin gene were found to be expressed in barley. In silico analysis of relative abundance of the distinct tubulin sequences among ESTs derived from different libraries revealed that the various tubulin genes differed in their level of expression, and to some extent were tissue specific.

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Section: Introductionmentioning

confidence: 99%

Cloning and expression of the tubulin genes in barley

Radchuk

Sreenivasulu

Blume

et al. 2008

Cell Biology International

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“…In addition, cell wall-related hub genes (probable E3 ubiquitin- ligase HIP1 isoform X1, Sun et al., 2009 , exocyst complex component EXO70A1 Synek et al., 2006 ) ( Table S6 ), together with carbohydrate/sugar metabolism and transportation-related genes (malate synthase, glyoxysomal, acetate butyrate ligase AAE7, peroxisomal, Allen et al., 2011 , sugar transporter 7-like, which might contribute to the uptake and recycling of cell wall sugars, Rottmann et al., 2018 ) ( Table S6 ) were activated in C. atlantica seedlings after 20 days of drought. Other plant growth-related hub genes were, conversely, inhibited (probable purine permease 11, Qi and Xiong, 2013 ; kinesin KIN-14I, Cooper et al., 2003 ; beta-tubulin, Eckardt, 2006 ; probable strigolactone esterase DAD2, involved in plant shoot branching, Hamiaux et al., 2012 ; cellulose synthase E6, involved in synthesis of cell wall cellulose) ( Table S7 ), including plant polyamine biosynthesis-related genes (ornithine decarboxylase-like, Illingworth and Michael, 2012 ; Chen et al., 2019 ) ( Table S7 ).…”

Section: Discussionmentioning

confidence: 99%

De novo transcriptome sequencing and gene co-expression reveal a genomic basis for drought sensitivity and evidence of a rapid local adaptation on Atlas cedar (Cedrus atlantica)

et al. 2023

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IntroductionUnderstanding the adaptive capacity to current climate change of drought-sensitive tree species is mandatory, given their limited prospect of migration and adaptation as long-lived, sessile organisms. Knowledge about the molecular and eco-physiological mechanisms that control drought resilience is thus key, since water shortage appears as one of the main abiotic factors threatening forests ecosystems. However, our current background is scarce, especially in conifers, due to their huge and complex genomes.MethodsHere we investigated the eco-physiological and transcriptomic basis of drought response of the climate change-threatened conifer Cedrus atlantica. We studied C. atlantica seedlings from two locations with contrasting drought conditions to investigate a local adaptation. Seedlings were subjected to experimental drought conditions, and were monitored at immediate (24 hours) and extended (20 days) times. In addition, post-drought recovery was investigated, depicting two contrasting responses in both locations (drought resilient and non-resilient). Single nucleotide polymorphisms (SNPs) were also studied to characterize the genomic basis of drought resilience and investigate a rapid local adaptation of C. atlantica.ResultsDe novo transcriptome assembly was performed for the first time in this species, providing differences in gene expression between the immediate and extended treatments, as well as among the post-drought recovery phenotypes. Weighted gene co-expression network analysis showed a regulation of stomatal closing and photosynthetic activity during the immediate drought, consistent with an isohydric dynamic. During the extended drought, growth and flavonoid biosynthesis inhibition mechanisms prevailed, probably to increase root-to-shoot ratio and to limit the energy-intensive biosynthesis of secondary metabolites. Drought sensitive individuals failed in metabolism and photosynthesis regulation under drought stress, and in limiting secondary metabolite production. Moreover, genomic differences (SNPs) were found between drought resilient and sensitive seedlings, and between the two studied locations, which were mostly related to transposable elements.DiscussionThis work provides novel insights into the transcriptomic basis of drought response of C. atlantica, a set of candidate genes mechanistically involved in its drought sensitivity and evidence of a rapid local adaptation. Our results may help guide conservation programs for this threatened conifer, contribute to advance drought-resilience research and shed light on trees’ adaptive potential to current climate change.

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“…In addition, for tiger nut tuber tissues, TUB4 and UCE2 are a pair of recommended RGs. Tubulin, encoded by TUB [ 67 , 68 ], is the main component of plant microtubules [ 69 ], which can maintain cell morphology [ 70 ], promote intracellular transport [ 71 ], and participate in cell elongation [ 72 ], movement, and cell division [ 73 , 74 , 75 ].…”

Section: Discussionmentioning

confidence: 99%

Selection and Validation of Reference Genes for qRT-PCR Analysis in the Oil-Rich Tuber Crop Tiger Nut (Cyperus esculentus) Based on Transcriptome Data

Bai

Chen

et al. 2021

IJMS

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Tiger nut (Cyperus esculentus), a perennial C4 plant of the Cyperaceae family, is an unconventional crop that is distinguished by its oil-rich tubers, which also possesses the advantages of strong resistance, wide adaptability, short life periods, and large biomass. To facilitate studies on gene expression in this species, we identified and validated a series of reference genes (RGs) based on transcriptome data, which can be employed as internal controls for qRT-PCR analysis in tiger nut. Fourteen putative candidate RGs were identified and evaluated across nine different tissues of two cultivars, and the RGs were analyzed using three different algorithms (geNorm, NormFinder, and BestKeeper). The stability rankings of the candidate RGs were merged into consensus lists with RankAggreg. For the below-ground storage organ of tiger nut, the optimal RGs were TUB4 and UCE2 in different developmental stages of tubers. UCE2 and UBL5 were the most stably expressed RGs among all tissues, while Rubisco and PGK exhibited the lowest expression stability. UCE2, UBL5 and Rubisco were compared to normalize the expression levels of the caleosin (CLO) and diacylglycerol acyltransferase 2-2 (DGAT2-2) genes across the same tissues. Our results showed that the RGs identified in this study, which exhibit more uniform expression patterns, may be utilized for the normalization of qRT-PCR results, promoting further research on gene expression in various tissues of tiger nut.

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Function of γ-Tubulin in Plants

Cited by 7 publications

References 17 publications

Cloning and expression of the tubulin genes in barley

Cloning and expression of the tubulin genes in barley

De novo transcriptome sequencing and gene co-expression reveal a genomic basis for drought sensitivity and evidence of a rapid local adaptation on Atlas cedar (Cedrus atlantica)

Selection and Validation of Reference Genes for qRT-PCR Analysis in the Oil-Rich Tuber Crop Tiger Nut (Cyperus esculentus) Based on Transcriptome Data

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