Function, Structure, and Evolution of Flavonoid Glycosyltransferases in Plants

Yonekura‐Sakakibara, Keiko; Saito, Kazuki

doi:10.1002/9781118329634.ch3

Cited by 13 publications

(14 citation statements)

References 71 publications

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“…Previous phylogenetic analyses have categorized flavonoid UGTs into unique clusters based on their regiospecifity (i.e., the position of glycosylation) for sugar acceptors, including 3GT, 5GT, 7GT, 3′GT and GGT subfamilies 14 . To figure out which group our putative UGTs belong to, we generated a phylogenetic tree by neighbor-joining algorithm using amino acid sequences of the 29 previously reported flavonoid UGTs and the candidates identified in this study (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Widely distributed in the plant kingdom, flavonoids usually exist in their decorated forms catalyzed by glycosyltransferases, acyltransferases, hydroxylases, and methyltransferases 13 , 14 . Glycosylation of flavonoids confers structural complexity, as well as molecular solubility and stability, subcellular transportability, and biological activity.…”

Section: Introductionmentioning

confidence: 99%

“…They are largely characterized by a C -terminus signature motif compromising 44 amino acid residues (Plant Secondary Product Glycosyltransferase (PSPG) box) 24 . A number of flavonoid UGTs have been characterized in a wide range of plant species, including crops, ornamental and medical plants 14 . These UGTs include flavonoid 3- O -glycosyltransferases (3GTs), flavonoid 5- O -glycosyltransferases (5GTs), flavonoid 7- O -glycosyltransferases (7GTs), flavonoid 3′- O -glycosyltransferases (3′GTs), flavonoid glycoside glycosyltransferases (GGTs) and flavonoid C -glycosyltransferases (CGTs).…”

Section: Introductionmentioning

confidence: 99%

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Differentially evolved glucosyltransferases determine natural variation of rice flavone accumulation and UV-tolerance

et al. 2017

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Decoration of phytochemicals contributes to the majority of metabolic diversity in nature, whereas how this process alters the biological functions of their precursor molecules remains to be investigated. Flavones, an important yet overlooked subclass of flavonoids, are most commonly conjugated with sugar moieties by UDP-dependent glycosyltransferases (UGTs). Here, we report that the natural variation of rice flavones is mainly determined by OsUGT706D1 (flavone 7-O-glucosyltransferase) and OsUGT707A2 (flavone 5-O-glucosyltransferase). UV-B exposure and transgenic evaluation demonstrate that their allelic variation contributes to UV-B tolerance in nature. Biochemical characterization of over 40 flavonoid UGTs reveals their differential evolution in angiosperms. These combined data provide biochemical insight and genetic regulation into flavone biosynthesis and additionally suggest that adoption of the positive alleles of these genes into breeding programs will likely represent a potential strategy aimed at producing stress-tolerant plants.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Differentially evolved glucosyltransferases determine natural variation of rice flavone accumulation and UV-tolerance

et al. 2017

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“…Flavones usually exist in their decorated forms, which are produced by the activities of glycosyltransferases, acyltransferases, hydroxylases, and methyltransferases [42,43]. Glycosylation of flavones confers structural complexity and different biological activities [35].…”

Section: Discussionmentioning

confidence: 99%

Flavones Produced by Mulberry Flavone Synthase Type I Constitute a Defense Line against the Ultraviolet-B Stress

Yang

et al. 2020

Plants

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Flavones, one of the largest classes of flavonoids in plants, have a variety of bioactivities and participate in the resistance response of plants to biotic and abiotic stresses. However, flavone synthase (FNS), the key enzyme for flavone biosynthesis, has not yet been characterized in mulberry. In this study, we report that the leaves of certain mulberry cultivars, namely BJ7, PS2, and G14, are rich in flavones. We identified a Fe2+/2-oxoglutarate-dependent dioxygenase from Morus notabilis (MnFNSI) that shows the typical enzymatic activity of a FNSI-type enzyme, and directly converts eriodictyol and naringenin into their corresponding flavones. Overexpression of MnFNSI in tobacco increased the flavones contents in leaves and enhanced the tolerance of tobacco to ultraviolet-B (UV-B) stress. We found that mulberry cultivars with higher flavones contents exhibit less UV-B induced damage after a UV-B treatment. Accordingly, our findings demonstrate that MnFNSI, a FNSI-type enzyme, is involved in the biosynthesis of flavones, which provide protection against UV-B radiation. These results lay the foundation for obtaining mulberry germplasm resources that are more tolerant to UV-B stress and richer in their nutritional value.

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“…As in the case of flavonoids, the greatest source of chemical diversity is the number and position of sugars for glycosylation. Acylation is another main biochemical mechanism leading to diverse anthocyanin molecules in Arabidopsis [14,15]. Up to date, several enzymes have been characterized to catalyze these acylation reactions, using either malonyl-CoA or p-coumaroyl-CoA as substrates to transfer the malonyl or p-coumaroyl groups to cyanin structures [16].…”

Section: Chemical Structurementioning

confidence: 99%

The Flavonol-Anthocyanin Pathway in Blackberry and Arabidopsis: State of the Art

Gutiérrez¹,

García‐Villaraco²,

AntonioLucas³

et al. 2017

Flavonoids - From Biosynthesis to Human Health

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Flavonols and anthocyanins are plant secondary metabolites with an increasing interest due to their beneficial effects on human health. They are present in all plants, participating in plant protection against biotic and abiotic stresses. However, only some plant species accumulate them in relevant amounts, as is the case for berries. Among the health benefits reported is prevention of metabolic syndrome, s, including prevention of insulin resistance associated to type 2 diabetes. Therefore, there is a big interest to improve contents on plant foods to benefit health through the diet, as well as to obtain them for functional ingredients for food supplements. In fulfillment of this objective, a deep study about their biosynthetic pathway has been carried out in model plants, where the genome is available. However, not all species that accumulate them in high amounts have their genome sequenced, as is the case for blackberry. Transcriptomic approaches have been undertaken to gain knowledge of its specific biosynthetic pathway and regulatory elements, aiming to improve bioactive contents in the edible parts. Furthermore, determining the regulatory pathways will help to improve yields and in vitro production. For this purpose, a review on elicitors used to trigger this pathway is presented.

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Function, Structure, and Evolution of Flavonoid Glycosyltransferases in Plants

Cited by 13 publications

References 71 publications

Differentially evolved glucosyltransferases determine natural variation of rice flavone accumulation and UV-tolerance

Differentially evolved glucosyltransferases determine natural variation of rice flavone accumulation and UV-tolerance

Flavones Produced by Mulberry Flavone Synthase Type I Constitute a Defense Line against the Ultraviolet-B Stress

The Flavonol-Anthocyanin Pathway in Blackberry and Arabidopsis: State of the Art

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