Function/structure correlation in novel pH-dependent cationic liposomes for glioma cells transfection in vitro

Abstract

Cationic liposomes are good candidates as gene carriers in cell biology due to their ability to bind DNA through electrostatic interactions. These liposomes are used as non-viral delivery systems in the gene therapy of glioma. pH-dependency and transfection efficiency of seven novel lipids (MORF-1, MORF-2, MORF-3, PIPR-1, PIPR-2, MM54 and DC-Amy) were studied. Two of these molecules (PIPR-2 and MM54) show at specific charge ratios better transfection efficiency than that of some commercially available liposomes.

A novel method to obtain chitosan/DNA nanospheres and a study of their release properties

“…In order to investigate in more detail the interaction properties of chitosan/DNA nanospheres, gel retardation assays were performed following standard procedures. Samples containing 2 mg of chitosan/DNA nanoparticles in PBS were mixed with loading buffer, loaded into 0.8% agarose gel (in TBE buffer), and run for 45 min at 100 V. We previously reported that polyamine polymers and cationic liposomes bearing amino groups on their lipid heads efficiently interact with DNA, leading to hindered adducts unable to migrate on agarose gel [25,34]. Figure 5 shows the migration of free hsDNA (lanes 1-3) and of chitosan/DNA nanospheres (lanes 4-6).…”

Synthesis of novel cationic lipids for preparation of liposomes to be used in gene therapy of glioma

“…The dimensions were measured by a light scattering apparatus and are suitable for transfection experiments [7]. In the dynamic light scattering technique, we measure the normalized time autocorrelation of the intensity of the scattered light which, for a dilute suspension of monodisperse particles decays exponentially with a decay rate G = 2 q 2D , where q is the magnitude of the scattering wave vector and D the diffusion coefficient.…”

“…The cationic lipid 6e (code name: MM54) resulted to be the most interesting one for its transfection good properties [7] in C6 glyoma cells.…”

Intracerebral Diffusion of Paramagnetic Cationic Liposomes Containing Gd(DTPA)2- Followed by MRI Spectroscopy: Assessment of Patternc Diffusion and Time Steadiness of a Non-Viral Vector Model

“…with a non-invasive technique, the diffusion of the contrast agent and, as a consequence, the therapeutic drugs that may be introduced into the liposome cavity Several cationic liposomes were recently reported in the literature as being efficient delivery vehicles in vitro and in vivo. One of the most common preparations is the DOTAP/DOPE (I: I) mixture owing to the easy availability of the starting lipids, their low toxicity and the good and wellcharacterized transfection efficiency towards C6 cells (rat glioma cells) (32). The thin film hydration method allowed us to easily incorporate the contrast agent Gd(DTPA)2-into the liposome cavity during the lipid swelling time and to control the operating temperature well above the transition point of the system.…”