Functional Alteration and Differential Expression of the Bitter Taste Receptor T2R38 in Human Paranasal Sinus in Patients with Chronic Rhinosinusitis

Takemoto, Kota; Lomude, Luga Santo; Takeno, Sachio; Kawasumi, Tomohiro; Okamoto, Yukako; Hamamoto, Takao; Ishino, Takashi; Ando, Yuki; Ishikawa, Chie; Ueda, Tsutomu

doi:10.3390/ijms24054499

Cited by 8 publications

(4 citation statements)

References 61 publications

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“…K2P potassium channels including KCNK1 (TWIK-1), KCNK2 (TREK-1), KCNK5 (TASK-2) and KCNK6 (TWIK-2) were expressed in respiratory epithelial cells and modulated Na + absorption and Cl secretion in epithelial cells, which are the main components of ASL ( Zhao et al, 2012 ; Schwingshackl et al, 2013 ). The bitter taste receptor has been shown to play a role in the pathogenesis of CRS by enhancing upper airway innate immunity ( Takemoto et al, 2023 ). In polarized sinonasal epithelial cells of CRS patients, the bitter taste receptor agonist, denatonium, decreases the epithelial K2P current through a cAMP-dependent signaling pathway ( Kohanski et al, 2021 ).…”

Section: The Role Of Potassium Ion Channels In Mucosal Remodeling Of ...mentioning

confidence: 99%

The role of potassium ion channels in chronic sinusitis

Ding,

Gai,

et al. 2024

Front. Pharmacol.

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Chronic sinusitis is a common inflammatory disease of the nasal and sinus mucosa, leading to symptoms such as nasal congestion, runny nose, decreased sense of smell, and headache. It often recurs and seriously affects the quality of life of patients. However, its pathological and physiological mechanisms are not fully understood. In recent years, the role of potassium ion channels in the regulation of mucosal barrier function and inflammatory cell function has received increasing attention. In chronic sinusitis, there are often changes in the expression and function of potassium channels, leading to mucosal damage and a stronger inflammatory response. However, the related research is still in its early stages. This article will review the role of the potassium channel in the pathological and physiological changes of chronic sinusitis. The studies revealed that BK/TREK-1 potassium channel play a protective role in the nasal mucosal function through p38-MAPK pathway, and KCa3.1/Kv1.3 enhance the inflammatory response of Chronic rhinosinusitis by regulating immune cell function, intracellular Ca2+ signaling and ERK/MAPK/NF-κB pathway. Because ion channels are surface proteins of cell membranes, they are easier to intervene with drugs, and the results of these studies may provide new effective targets for the prevention and treatment of chronic sinusitis.

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Section: The Role Of Potassium Ion Channels In Mucosal Remodeling Of ...mentioning

confidence: 99%

The role of potassium ion channels in chronic sinusitis

Ding,

Gai,

et al. 2024

Front. Pharmacol.

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“…Following the results described above, a growing number of studies have continued to examine the relationship between TASR38 polymorphisms and CRS [ 194 , 195 , 196 , 197 , 198 , 199 , 200 , 201 ]. One study conducted in an Italian population did not find a correlation between CRS and TAS2R38 polymorphisms [ 194 ].…”

Section: Interactions Of T2rs With Pseudomonas Aeruginosa ...mentioning

confidence: 99%

Interkingdom Detection of Bacterial Quorum-Sensing Molecules by Mammalian Taste Receptors

Kouakou

Lee

2023

Microorganisms

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Bitter and sweet taste G protein-coupled receptors (known as T2Rs and T1Rs, respectively) were originally identified in type II taste cells on the tongue, where they signal perception of bitter and sweet tastes, respectively. Over the past ~15 years, taste receptors have been identified in cells all over the body, demonstrating a more general chemosensory role beyond taste. Bitter and sweet taste receptors regulate gut epithelial function, pancreatic β cell secretion, thyroid hormone secretion, adipocyte function, and many other processes. Emerging data from a variety of tissues suggest that taste receptors are also used by mammalian cells to “eavesdrop” on bacterial communications. These receptors are activated by several quorum-sensing molecules, including acyl-homoserine lactones and quinolones from Gram-negative bacteria such as Pseudomonas aeruginosa, competence stimulating peptides from Streptococcus mutans, and D-amino acids from Staphylococcus aureus. Taste receptors are an arm of immune surveillance similar to Toll-like receptors and other pattern recognition receptors. Because they are activated by quorum-sensing molecules, taste receptors report information about microbial population density based on the chemical composition of the extracellular environment. This review summarizes current knowledge of bacterial activation of taste receptors and identifies important questions remaining in this field.

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“…We divided the surgical specimens and either immersed them in RNAlater™ solution (Thermo Fisher Scientific, Waltham, MA, USA) for reverse transcription-polymerase chain reaction (RT-PCR) or fixed them in 4% paraformaldehyde for immunohistochemistry. A quantitative PCR analysis was performed on an ABI Prism 7300 system (Applied Biosystems, Foster City, CA, USA), as described in [16]. Cellular RNA was isolated using RNeasy mini-kits (Qiagen, Valencia, CA, USA).…”

Section: Quantitative Rt-pcr Analysismentioning

confidence: 99%

Oncostatin M’s Involvement in the Pathogenesis of Chronic Rhinosinusitis: Focus on Type 1 and 2 Inflammation

Ishikawa,

Takeno,

Okamoto

et al. 2023

Biomedicines

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Objectives: The cytokine oncostatin M (OSM) elicits pathogenic effects involving disruption of the epithelial barrier function as a part of immunological response networks. It is unclear how these integrated cytokine signals influence inflammation and other physiological processes in the pathology of chronic rhinosinusitis (CRS). We investigated the expression and distribution of OSM and OSM receptor (OSMR) in CRS patients’ sinonasal specimens, and we compared the results with a panel of inflammatory cytokine levels and clinical features. Patients and Methods: We classified CRS patients as eosinophilic (ECRS, n = 36) or non-eosinophilic (non-ECRS, n = 35) based on the Japanese Epidemiological Survey of Refractory Eosinophilic Chronic Rhinosinusitis phenotypic criteria and compared their cases with those of 20 control subjects. We also examined OSM’s stimulatory effects on cytokine receptor expression levels using the human bronchial epithelium cell line BEAS-2B. Results: RT-PCR showed that the OSM mRNA levels were significantly increased in the CRS patients’ ethmoid sinus mucosa. The OSM mRNA levels were positively correlated with those of TNF-α, IL-1β, IL-13, and OSMR-β. In BEAS-2B cells, OSM treatment induced significant increases in the OSMRβ, IL-1R1, and IL-13Ra mRNA levels. Conclusions: OSM is involved in the pathogenesis of CRS in both type 1 and type 2 inflammation, suggesting the OSM signaling pathway as a potential therapeutic target for modulating epithelial stromal interactions.

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Functional Alteration and Differential Expression of the Bitter Taste Receptor T2R38 in Human Paranasal Sinus in Patients with Chronic Rhinosinusitis

Cited by 8 publications

References 61 publications

The role of potassium ion channels in chronic sinusitis

The role of potassium ion channels in chronic sinusitis

Interkingdom Detection of Bacterial Quorum-Sensing Molecules by Mammalian Taste Receptors

Oncostatin M’s Involvement in the Pathogenesis of Chronic Rhinosinusitis: Focus on Type 1 and 2 Inflammation

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