Functional analysis and subcellular location of two flavohemoglobins from Aspergillus oryzae

Zhou, Shengmin; Fushinobu, Shinya; Kim, Sang Wan; Nakanishi, Yoshito; Maruyama, Jun‐ichi; Kitamoto, Katsuhiko; Wakagi, Takayoshi; Shoun, Hirofumi

doi:10.1016/j.fgb.2010.08.011

Cited by 32 publications

(26 citation statements)

References 52 publications

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“…In a wide range of organisms, these flavohaemoglobins detoxify NO (Takaya et al, 1997;Gardner et al, 1998;Baidya et al, 2011). The in vivo impact of these enzymes was further demonstrated for relatives of A. fumigatus, namely A. nidulans and A. oryzae, as respective null mutants for orthologues of AFUB099650 became more sensitive to NO generating compounds like DETA-NO or NO 2 − (Schinko et al, 2010;Zhou et al, 2011). Although a similar function was not readily expected for FglA, we also analysed the growth of the mutant in the presence of NO.…”

Section: Fungoglobins Contribute To Growth In Low-oxygen Environmentsmentioning

confidence: 87%

The novel globin protein fungoglobin is involved in low oxygen adaptation of Aspergillus fumigatus

Hillmann

Linde

Beckmann

et al. 2014

Molecular Microbiology

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SummaryThe human pathogenic fungus Aspergillus fumigatus normally lives as a soil saprophyte. Its environment includes poorly oxygenated substrates that also occur during tissue invasive growth of the fungus in the human host. Up to now, few cellular factors have been identified that allow the fungus to efficiently adapt its energy metabolism to hypoxia. Here, we cultivated A. fumigatus in an O2-controlled fermenter and analysed its responses to O2 limitation on a minute timescale. Transcriptome sequencing revealed several genes displaying a rapid and highly dynamic regulation. One of these genes was analysed in detail and found to encode fungoglobin, a previously uncharacterized member of the sensor globin protein family widely conserved in filamentous fungi. Besides low O2, iron limitation also induced transcription, but regulation was not entirely dependent on the two major transcription factors involved in adaptation to iron starvation and hypoxia, HapX and SrbA respectively. The protein was identified as a functional haemoglobin, as binding of this cofactor was detected for the recombinant protein. Gene deletion in A. fumigatus confirmed that haem-binding fungoglobins are important for growth in microaerobic environments with O 2 levels far lower than in hypoxic human tissue.

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Section: Fungoglobins Contribute To Growth In Low-oxygen Environmentsmentioning

confidence: 87%

The novel globin protein fungoglobin is involved in low oxygen adaptation of Aspergillus fumigatus

Hillmann

Linde

Beckmann

et al. 2014

Molecular Microbiology

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“…In addition, some A. oryzae genes for NO metabolism, namely a mitochondrial copper-containing nitrite reductase (nirK), a NO reductase (nicA), and a cytosolic NO-detoxifying flavohemoprotein (fhb1) were also up-regulated at low O 2 levels (Kaya et al 2004;Nakanishi et al 2010;Zhou et al 2011). These findings lead the authors to speculate about a possible role of fungal denitrification (reduction of nitrate to nitrous oxide) in hypoxic survival and NAD + recycling of A. oryzae.…”

Section: Gene and Protein Expression During Hypoxiamentioning

confidence: 97%

Insights into the cellular responses to hypoxia in filamentous fungi

2015

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Most eukaryotes require molecular oxygen for growth. In general, oxygen is the terminal electron acceptor of the respiratory chain and represents an important substrate for the biosynthesis of cellular compounds. However, in their natural environment, such as soil, and also during the infection, filamentous fungi are confronted with low levels of atmospheric oxygen. Transcriptome and proteome studies on the hypoxic response of filamentous fungi revealed significant alteration of the gene expression and protein synthesis upon hypoxia. These analyses discovered not only common but also species-specific responses to hypoxia with regard to NAD(+) regeneration systems and other metabolic pathways. A surprising outcome was that the induction of oxidative and nitrosative stress defenses during oxygen limitation represents a general trait of adaptation to hypoxia in many fungi. The interplay of these different stress responses is poorly understood, but recent studies have shown that adaptation to hypoxia contributes to virulence of pathogenic fungi. In this review, results on metabolic changes of filamentous fungi during adaptation to hypoxia are summarized and discussed.

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“…NO at low concentrations protects Saccharomyces cerevisiae against heavy metal Cu 2+ toxicity, while high concentrations of NO have opposite effects (Chiang et al, 2000;Turrion-Gomez et al, 2010;Zhou et al, 2011). Also, low levels of NO show a cytoprotective effect on S. cerevisiae during heat shock stress and high hydrostatic pressure (Domitrovic et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Nitric oxide alleviates heat stress-induced oxidative damage in Pleurotus eryngii var. tuoliensis

WeiWei

Huang

Chen

et al. 2012

Fungal Genetics and Biology

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Functional analysis and subcellular location of two flavohemoglobins from Aspergillus oryzae

Cited by 32 publications

References 52 publications

The novel globin protein fungoglobin is involved in low oxygen adaptation of Aspergillus fumigatus

The novel globin protein fungoglobin is involved in low oxygen adaptation of Aspergillus fumigatus

Insights into the cellular responses to hypoxia in filamentous fungi

Nitric oxide alleviates heat stress-induced oxidative damage in Pleurotus eryngii var. tuoliensis

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