Functional Analysis of a Wilt Fungus Inducible &amp;lt;i&amp;gt;PR&amp;lt;/i&amp;gt;10-1 Gene from Cotton

Chen, Lijun; Sun, Na; Wang, Jin; Ling, Hua; Zhang, Lida; Zuo, Kaijing

doi:10.4236/ajps.2013.42a054

Cited by 5 publications

(4 citation statements)

References 32 publications

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“…The in vitro antimicrobial activities of most PRs are due to their hydrolytic effects on cell walls, contact toxicity and perhaps their involvement in defense signaling. Moreover, some PRs may function as RNase, suggesting they may degrade fungal RNA during infections ( Chen et al, 2013 ). For example, the recombinant LrPR4 protein expressed in Escherichia coli shows RNase activity toward hydrolyzing RNA from Lycoris radiata bulbs and has antifungal activity against rice ( Oryza sativa ) blast Magnaporthe grisea ( Li et al, 2009 ).…”

Section: Introductionmentioning

confidence: 99%

“…An earlier investigation confirmed the in vitro RNase activity of soybean ( Glycine max ) GmPRP, which is a PR that can inhibit Phytophthora sojae mycelial growth ( Xu et al, 2014 ). Another study proved that GbPR10-1 from sea island cotton ( Gossypium barbadense ) adversely affects V. dahliae mycelial growth and shows in vitro RNase activity ( Chen et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

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A Pathogenesis-Related Protein-Like Gene Is Involved in the Panax notoginseng Defense Response to the Root Rot Pathogen

Wang

Tang

et al. 2021

Front. Plant Sci.

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Pathogenesis-related proteins (PRs) are a class of proteins that accumulate in response to biotic and abiotic stresses to protect plants from damage. In this study, a gene encoding a PR-like protein (PnPR-like) was isolated from Panax notoginseng, which is used in traditional Chinese herbal medicines. An analysis of gene expression in P. notoginseng indicated that PnPR-like was responsive to an infection by the root rot pathogen Fusarium solani. The expression of this gene was induced by several signaling molecules, including methyl jasmonate, ethephon, hydrogen peroxide, and salicylic acid. The PnPR-like-GFP fusion gene was transiently expressed in onion (Allium cepa) epidermal cells, which revealed that PnPR-like is a cytoplasmic protein. The purified recombinant PnPR-like protein expressed in Escherichia coli had antifungal effects on F. solani and Colletotrichum gloeosporioides as well as inhibited the spore germination of F. solani. Additionally, the in vitro ribonuclease (RNase) activity of the recombinant PnPR-like protein was revealed. The PnPR-like gene was inserted into tobacco (Nicotiana tabacum) to verify its function. The gene was stably expressed in T2 transgenic tobacco plants, which exhibited more RNase activity and greater disease resistance than the wild-type tobacco. Moreover, the transient expression of hairpin RNA targeting PnPR-like in P. notoginseng leaves increased the susceptibility to F. solani and decreased the PnPR-like expression level. In conclusion, the cytoplasmic protein PnPR-like, which has RNase activity, is involved in the P. notoginseng defense response to F. solani.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Pathogenesis-Related Protein-Like Gene Is Involved in the Panax notoginseng Defense Response to the Root Rot Pathogen

Wang

Tang

et al. 2021

Front. Plant Sci.

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“…after fungal infection during induction of resistance. In cotton, early stage expression of PR-10 combined with phytoalexin production contributes to Verticillium wilt resistance [15]. PR-10 proteins are encoded by multigene families and are constitutively expressed in different organs.…”

Section: Introductionmentioning

confidence: 99%

Induction of PR-10 genes and metabolites in strawberry plants in response to Verticillium dahliae infection

2019

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Background The soil-borne vascular pathogen Verticillium dahliae causes severe wilt symptoms in a wide range of plants including strawberry ( Fragaria × ananassa). To enhance our understanding of the effects of V. dahliae on the growth and development of F. × ananassa, the expression patterns of 21 PR-10 genes were investigated by qPCR analysis and metabolite changes were determined by LC-MS in in vitro F. × ananassa plants upon pathogen infection. Results The expression patterns of the 21 isoforms showed a wide range of responses. Four PR-10 genes were highly induced in leaves upon pathogen infection while eight members were significantly up-regulated in roots. A simultaneously induced expression in leaves and roots was detected for five PR-10 genes. Interestingly, two isoforms were expressed upon infection in all three tissues (leaves, roots and stems) while no induction was detected for two other members. Accumulation of antifungal catechin and epicatechin was detected upon pathogen infection in roots and stems at late stages, while caffeic acid and citric acid were observed only in infected roots. Production of abscisic acid, salicylic acid, jasmonic acid (JA), gibberellic acid and indole acetic acid (IAA) was induced in infected leaves and stems at early stages. IAA and JA were the sole hormones to be ascertained in infected roots at late stages. Conclusions The induction of several PR-10 genes upon infection of strawberry plants with V. dahliae suggest a role of PR-10 genes in the defense response against this pathogen. Production of phytohormones in the early stages of infection and antifungal metabolites in late stages suppose that they are implicated in this response. The results may possibly improve the control measures of the pathogen. Electronic supplementary material The online version of this article (10.1186/s12870-019-1718-x) contains supplementary material, which is available to authorized users.

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“…Previous studies have shown that PR10 proteins can degrade pathogen RNA as a result of their Rnase activity (Bantignies et al, 2000;Zhou et al, 2002). A GbPR10-1 protein from G. barbadense has Rnase activity and can restrict the hyphal growth of V. dahliae (Chen et al, 2013). Previous studies have shown that JAs alter the accumulation of lignins and phenolics (Ali et al, 2007;Denness et al, 2011), but the molecular GhJAZ2-GhbHLH171 mediates cotton defence responses mechanism is unknown.…”

Section: Discussionmentioning

confidence: 99%

GhJAZ2 attenuates cotton resistance to biotic stresses via the inhibition of the transcriptional activity of GhbHLH171

Zhu

et al. 2017

Molecular Plant Pathology

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Plants have evolved effective mechanisms to protect themselves against multiple stresses, and employ jasmonates (JAs) as vital defence signals to defend against pathogen infection. The accumulation of JA, induced by signals from biotic and abiotic stresses, results in the degradation of Jasmonate-ZIM-domain (JAZ) proteins, followed by the de-repression of JAZ-repressed transcription factors (such as MYC2) to activate defence responses and developmental processes. Here, we characterized a JAZ family protein, GhJAZ2, from cotton (Gossypium hirsutum) which was induced by methyl jasmonate (MeJA) and inoculation of Verticillium dahliae. The overexpression of GhJAZ2 in cotton impairs the sensitivity to JA, decreases the expression level of JA-response genes (GhPDF1.2 and GhVSP) and enhances the susceptibility to V. dahliae and insect herbivory. Yeast two-hybrid and bimolecular fluorescence complementation assays showed that GhJAZ2 may be involved in the regulation of cotton disease resistance by interaction with further disease-response proteins, such as pathogenesis-related protein GhPR10, dirigent-like protein GhD2, nucleotide-binding site leucine-rich repeat (NBS-LRR) disease-resistant protein GhR1 and a basic helix-loop-helix transcription factor GhbHLH171. Unlike MYC2, overexpression of GhbHLH171 in cotton activates the JA synthesis and signalling pathway, and improves plant tolerance to the fungus V. dahliae. Molecular and genetic evidence shows that GhJAZ2 can interact with GhbHLH171 and inhibit its transcriptional activity and, as a result, can restrain the JA-mediated defence response. This study provides new insights into the molecular mechanisms of GhJAZ2 in the regulation of the cotton defence response.

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Functional Analysis of a Wilt Fungus Inducible <i>PR</i>10-1 Gene from Cotton

Cited by 5 publications

References 32 publications

A Pathogenesis-Related Protein-Like Gene Is Involved in the Panax notoginseng Defense Response to the Root Rot Pathogen

A Pathogenesis-Related Protein-Like Gene Is Involved in the Panax notoginseng Defense Response to the Root Rot Pathogen

Induction of PR-10 genes and metabolites in strawberry plants in response to Verticillium dahliae infection

GhJAZ2 attenuates cotton resistance to biotic stresses via the inhibition of the transcriptional activity of GhbHLH171

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