Functional analysis of differentially expressed proteins in Chinese bayberry ( Myrica rubra Sieb. et Zucc.) fruits during ripening

Chen, Yiyong; Zhang, Zehuang; Zhong, Can-Yu; Song, Xiaomin; Lin, Qihua; Chun-mei, Huang; Rong-hui, Huang; Chen, Wei

doi:10.1016/j.foodchem.2015.06.023

Cited by 12 publications

(10 citation statements)

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“…At the same time, many previous studies indicated that plant ADHs have been involved in seedling and pollen development as well as fruit development [ 40 , 41 ]. For example, ADH was markedly increased at the pink and red fruit stages in ripening Chinese bayberry [ 27 ] and tomato [ 42 ] fruits. In contrast, the ADH protein decreased significantly in strawberry fruits at different ripening stages [ 43 ], which was not the same as our results.…”

Section: Discussionmentioning

confidence: 99%

“…Two varieties of peach fruit had differential protein expression for 53 proteins before and after climacteric conditions, and these proteins were related to basic metabolism, secondary metabolism, ethylene synthesis and the stress response [ 26 ]. The waxberry had 43 differentially expressed proteins at different developmental periods that were mainly related to the metabolism of sugar and energy, anthocyanin metabolism and the stress response as well as defence and other properties [ 27 ]. The oriental melon is a typical climacteric fruit; however there are limited reports on the proteomics for oriental melons at different ripening stages.…”

Section: Introductionmentioning

confidence: 99%

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iTRAQ-based Protein Profiling and Fruit Quality Changes at Different Development Stages of Oriental Melon

Guo

Cui

et al. 2017

BMC Plant Biol

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BackgroundOriental melon is one of the most popular crops for its nutritional and flavour quality. Components that determine melon quality, such as sugar, colour, texture, flavour and aroma, among other factors, accumulate in different developmental stages. Thus, correlating the proteomic profiles with the biochemical and physiological changes occurring in the oriental melon is very important for advancing our understanding of oriental melon quality in the ripening processes.ResultsiTRAQ-based protein profiling was conducted on ‘YuMeiren’ oriental melon fruit at different developmental stages. Physiological quality indices, including firmness, rind colour, soluble solids content (SSC), ethylene production, sugar content and volatile compounds were also characterized during four maturity periods of the melon, including 5, 15, 25 and 35 days after anthesis (DAA). A principal component analysis (PCA) revealed that the aroma volatiles at 5 DAA and 15 DAA were similar and separated from that of 35 DAA. More than 5835 proteins were identified and quantified in the two biological repeats and divided into 4 clusters by hierarchical cluster analysis. A functional analysis was performed using Blast2GO software based on the enrichment of a GO analysis for biological process, molecular function and cellular components. The main KEGG pathways, such as glycolysis, α-linolenic acid and starch and sucrose metabolism, were analyzed.The gene family members corresponding to differentially expressed proteins, including lipoxygenase (CmLOX01-18) and alcohol acetyltransferase (CmAAT1-4) involved in the α-linolenic acid metabolic pathway, were verified with real-time qPCR. The results showed that the expression patterns of 64.7% of the genes were consistent with the expression patterns of the corresponding proteins.ConclusionsThis study combined the variation of the quality index and differentially expressed proteins of oriental melon at different developmental stages that laid the foundation for the subsequent protein and gene function validation.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-017-0977-7) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

iTRAQ-based Protein Profiling and Fruit Quality Changes at Different Development Stages of Oriental Melon

Guo

Cui

et al. 2017

BMC Plant Biol

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“…In the KEGG reference map 00942 for the anthocyanin synthesis signaling pathway, the copy number of anthocyanidin 3- O -glucoside 5- O -glucosyltransferase ( UGT75C1 ; six copies) [28] and anthocyanidin 3- O -glucosyltransferase ( BZ1 ; five copies) [29] was high (Figure 4c; Additional file 1: Table S11). These two enzymes play important roles in anthocyanin synthesis [1, 30, 31]. The phylogenetic tree based on UGT75C1 confirmed tandem duplication in some genes (Figure 4d).…”

Section: Resultsmentioning

confidence: 99%

“…Regarding the terpenoid signaling pathway, the copy numbers of (3 S )-linalool synthase (5) [31] of the monoterpene pathway (Fig. 8a; Additional file 1: Table S11), cytochrome P450, 82 family, subfamily g, polypeptide 1 (CYP82G1) (6) of the diterpenes signaling pathway [38] (Fig.…”

Section: Resultsmentioning

confidence: 99%

Genome sequencing provides insights into the evolution and antioxidant activity of Chinese bayberry

Ren

Zhang

et al. 2019

BMC Genomics

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Background Chinese bayberry ( Myrica rubra Sieb. & Zucc.) is an economically important fruit tree characterized by its juicy fruits rich in antioxidant compounds. Elucidating the genetic basis of the biosynthesis of active antioxidant compounds in bayberry is fundamental for genetic improvement of bayberry and industrial applications of the fruit’s antioxidant components. Here, we report the genome sequence of a multiple disease-resistant bayberry variety, ‘Zaojia’, in China, and the transcriptome dynamics in the course of fruit development. Results A 289.92 Mb draft genome was assembled, and 26,325 protein-encoding genes were predicted. Most of the M. rubra genes in the antioxidant signaling pathways had multiple copies, likely originating from tandem duplication events. Further, many of the genes found here present structural variations or amino acid changes in the conserved functional residues across species. The expression levels of antioxidant genes were generally higher in the early stages of fruit development, and were correlated with the higher levels of total flavonoids and antioxidant capacity, in comparison with the mature fruit stages. Based on both gene expression and biochemical analyses, five genes, namely, caffeoyl-CoA O-methyltransferase, anthocyanidin 3-O-glucosyltransferase, (+)-neomenthol dehydrogenase, gibberellin 2-oxidase, and squalene monooxygenase, were suggested to regulate the flavonoid, anthocyanin, monoterpenoid, diterpenoid, and sesquiterpenoid/triterpenoid levels, respectively, during fruit development. Conclusions This study describes both the complete genome and transcriptome of M. rubra . The results provide an important basis for future research on the genetic improvement of M. rubra and contribute to the understanding of its genetic evolution. The genome sequences corresponding to representative antioxidant signaling pathways can help revealing useful traits and functional genes. Electronic supplementary material The online version of this article (10.1186/s12864-019-5818-7) contains supplementary material, which is available to authorized users.

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“…Depending on the cultivar, ripe bayberry fruits can be purple, red, pink, or white in color (Cheng, Chen, Chen, et al, 2015). The “ Bi qi ” bayberry with red or purple black colors we used in this study is the most popular cultivar in China markets because of its excellent sugar‐acid balance and healthy nutrients (Chen et al, 2016). Bayberry fruits are rich in phenolic compounds including anthocyanins and phenolic acids, which contribute to the high antioxidant activity in the fruits.…”

Section: Introductionmentioning

confidence: 99%

Chemometrics characterization of volatile changes in processed bayberry juice versus intact fruit during storage by headspace solid‐phase micro‐extraction combined with GC–MS

Fang

Chen

Tian

et al. 2020

J Food Process Preserv

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Chinese bayberry deteriorates rapidly after harvest. To gain a better understanding of flavor deterioration, we investigated the volatile changes in processed bayberry juice and intact fruits using HS–SPME/GC–MS during storage. The concentration of hexanal, which has a “grass” aroma, and other associated aldehydes significantly decreased (p < .05) during storage, and it is likely that these losses are responsible for the decrease in freshness. Ethanol (“alcoholic” note), ethyl acetate (“fruity” note), and acetic acid (“sour” note) were found to contribute to off‐flavor accumulation, and large increases were detected in both the bayberry juice and fruit. Meanwhile, increased concentrations of 3‐methyl‐1‐butanol, 1‐nonanol, ethyl hexanoate, and hexanoic acid were found in the bayberry juice. In contrast, storage of the intact fruit resulted in increased concentrations of terpinen‐4‐ol, methyl acetate, and γ‐terpinene. Practical applications GC–MS fingerprinting is useful for practical application to catch the volatile changes and freshness during postharvest storage of bayberry fruit and juice.

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Functional analysis of differentially expressed proteins in Chinese bayberry ( Myrica rubra Sieb. et Zucc.) fruits during ripening

Cited by 12 publications

References 39 publications

iTRAQ-based Protein Profiling and Fruit Quality Changes at Different Development Stages of Oriental Melon

iTRAQ-based Protein Profiling and Fruit Quality Changes at Different Development Stages of Oriental Melon

Genome sequencing provides insights into the evolution and antioxidant activity of Chinese bayberry

Chemometrics characterization of volatile changes in processed bayberry juice versus intact fruit during storage by headspace solid‐phase micro‐extraction combined with GC–MS

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