Functional analysis of ecdysteroid biosynthetic enzymes of the rice planthopper, Nilaparvata lugens

Zhou, Xiang; Ye, Yi-Zhou; Ogihara, Mari H.; Takeshima, Mika; Fujinaga, Daiki; Liu, Cheng-Wen; Zhu, Zhen; Kataoka, Hiroshi; Bao, Yan-Yuan

doi:10.1016/j.ibmb.2020.103428

Cited by 39 publications

(57 citation statements)

References 67 publications

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“…melanogaster , A . aegypti and Nilaparvata lugens has already been reported [ 91 – 94 ]. Ecdysteroid signaling involves the activation of a heterodimer receptor, composed of the ecdysone receptor (EcR) and the ultraspiracle protein (USP) [ 95 ].…”

Section: Resultsmentioning

confidence: 99%

“…Also, Ruegg et al [90] reported that hemolymph titres of ecdysteroids in R. prolixus females peak 5 days after a blood meal and that ovariectomy prevents that increase, demonstrating an influence by the OV on ecdysteroid production. Coincidently, ecdysteroid biosynthesis by the OV in D. melanogaster, A. aegypti and Nilaparvata lugens has already been reported [91][92][93][94]. Ecdysteroid signaling involves the activation of a heterodimer receptor, composed of the ecdysone receptor (EcR) and the ultraspiracle protein (USP) [95].…”

Section: Protein and Hormone Analysismentioning

confidence: 99%

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What happens after a blood meal? A transcriptome analysis of the main tissues involved in egg production in Rhodnius prolixus, an insect vector of Chagas disease

2020

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The blood-sucking hemipteran Rhodnius prolixus is a vector of Chagas disease, one of the most neglected tropical diseases affecting several million people, mostly in Latin America. The blood meal is an event with a high epidemiological impact since adult mated females feed several times, with each meal resulting in a bout of egg laying, and thereby the production of hundreds of offspring. By means of RNA-Sequencing (RNA-Seq) we have examined how a blood meal influences mRNA expression in the central nervous system (CNS), fat body and ovaries in order to promote egg production, focusing on tissue-specific responses under controlled nutritional conditions. We illustrate the cross talk between reproduction and a) lipids, proteins and trehalose metabolism, b) neuropeptide and neurohormonal signaling, and c) the immune system. Overall, our molecular evaluation confirms and supports previous studies and provides an invaluable molecular resource for future investigations on different tissues involved in successful reproductive events. These analyses serve as a starting point for new investigations, increasing the chances of developing novel strategies for vector population control by translational research, with less impact on the environment and more specificity for a particular organism.

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Section: Resultsmentioning

confidence: 99%

Section: Protein and Hormone Analysismentioning

confidence: 99%

What happens after a blood meal? A transcriptome analysis of the main tissues involved in egg production in Rhodnius prolixus, an insect vector of Chagas disease

2020

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“…We previously determined the 20E contents of N. lugens nymphs and adults [45]. In this study, 20E contents were measured in adult females after NlRpn and NlRac RNAi.…”

Section: Measurement Of 20e In Nilaparvata Lugens Adult Femalesmentioning

confidence: 99%

“…Briefly, newly emerged adult females were injected with each dsNlRpn or dsNlRac, with dsGFP-injected insects as controls. The 20E content was measured in the whole body at 120 h after emergence, according to Nakaoka et al [70] with some modifications, as described in Zhou et al [45].…”

Section: Measurement Of 20e In Nilaparvata Lugens Adult Femalesmentioning

confidence: 99%

Proteolytic activity of the proteasome is required for female insect reproduction

et al. 2021

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Non-ATPase regulatory subunits (Rpns) are components of the 26S proteasome involved in polyubiquitinated substrate recognition and deubiquitination in eukaryotes. Here, we identified 15 homologues sequences of Rpn and associated genes by searching the genome and transcriptome databases of the brown planthopper, Nilaparvata lugens , a hemipteran rice pest. Temporospatial analysis showed that NlRpn genes were significantly highly expressed in eggs and ovaries but were less-highly expressed in males. RNA interference-mediated depletion of NlRpn genes decreased the proteolytic activity of proteasome and impeded the transcription of lipase and vitellogenin genes in the fat bodies and ovaries in adult females, and reduced the triglyceride content in the ovaries. Decrease of the proteolytic activity of the proteasome via knockdown of NlRpn s also inhibited the transcription of halloween genes, including NlCYP307A2 , NlCYP306A2 and NlCYP314A1 , in the 20-hydroxyecdysone (20E) biosynthetic pathway in the ovaries, reduced 20E production in adult females, and impaired ovarian development and oocyte maturation, resulting in reduced fecundity. These novel findings indicate that the proteolytic activity of the proteasome is required for female reproductive processes in N. lugens , thus furthering our understanding of the reproductive and developmental strategies in insects.

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“…Much less is known about Nox in other insect species. Nilaparvata lugens is a model insect of Hemiptera because its whole genome sequence has been elucidated [10] and it possesses a highly RNAi-sensitive system [18,19]. In this study, by means of a deep search of the N. lugens genome and transcriptome databases, we identified two Nox sequences, which are the orthologs of human Nox4 and Nox5 genes, respectively.…”

Section: Introductionmentioning

confidence: 99%

NADPH Oxidase 5 Is Essential for Molting and Oviposition in a Rice Planthopper Nilaparvata lugens

Peng

Dai

Yang

et al. 2020

Insects

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The brown planthopper Nilaparvata lugens is a typical monophagous insect herbivore that feeds exclusively on rice sap. This insect pest causes serious damage to rice crops throughout East Asian countries. Chemical control remains the first choice for managing N. lugens populations; however, the use of insecticides has given rise to planthopper resurgence and additional environmental risks. Nilaparvata lugens is a model insect of Hemiptera because its whole genome sequence has been elucidated and is susceptible to RNA interference. In this study, our findings revealed that a superoxide-generating gene, NADPH oxidase 5 (Nox5), is essential for molting and oviposition in a Hemipteran insect Nilaparvata lugens. Knockdown of Nox5 transcript levels by RNA interference in 2nd–5th-instar nymphs results in significantly lethal deficits in the molting transitions from nymph–nymph and nymph–adult. Nox5 knockdown leads to a reduction of hydrogen peroxide in female ovaries and failure of oviposition from the insect ovipositor into the rice leaf sheath. Here, we provide in vivo evidence demonstrating that Nox5 is a key enzyme for regulating molting and oviposition in this insect species.

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Functional analysis of ecdysteroid biosynthetic enzymes of the rice planthopper, Nilaparvata lugens

Cited by 39 publications

References 67 publications

What happens after a blood meal? A transcriptome analysis of the main tissues involved in egg production in Rhodnius prolixus, an insect vector of Chagas disease

What happens after a blood meal? A transcriptome analysis of the main tissues involved in egg production in Rhodnius prolixus, an insect vector of Chagas disease

Proteolytic activity of the proteasome is required for female insect reproduction

NADPH Oxidase 5 Is Essential for Molting and Oviposition in a Rice Planthopper Nilaparvata lugens

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