Functional analysis of human memory B-cell subpopulations: IgD+CD27+ B cells are crucial in secondary immune response by producing high affinity IgM

Shi, Yan; Agematsu, Kazunaga; Ochs, Hans D.; Sugane, Kazuo

doi:10.1016/s1521-6616(03)00092-5

Cited by 146 publications

(127 citation statements)

References 44 publications

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“…The Ab genes of RV-specific intestinal-homing memory (␣ 4 ␤ 7 ϩ IgD Ϫ ) B cells in particular are almost completely devoid of somatic mutations, even though they are isotype switched (17). Our previous studies, however, considered IgD ϩ -positive cells to be naive cells, whereas recent studies show that a third major subset of B cells exists within the unclass-switched IgD ϩ population (i.e., CD27 ϩ IgD ϩ cells) that are memory cells (18,19). In the work here, we separately analyzed and compared the three major subjects of naive, unclass-switched memory, and class-switched memory cells from both RV-specific and randomly selected B cells.…”

Section: Discussionmentioning

confidence: 98%

“…Recent progress in lymphocyte-phenotyping methods has facilitated better definition of memory B cell subsets in humans. Circulating human B cells can be separated into at least three major naive or memory B cell groups: CD19 ϩ IgD ϩ CD27 Ϫ naive B cells, CD19 ϩ IgD ϩ CD27 ϩ unclass-switched memory B cells and CD19 ϩ IgD Ϫ CD27 ϩ class-switched memory B cells (18,19). Among these groups of B cells, CD19 ϩ IgD ϩ CD27 ϩ unclassswitched memory B cells are the most recently recognized population.…”

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confidence: 99%

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Immunodominance of the VH1–46 Antibody Gene Segment in the Primary Repertoire of Human Rotavirus-Specific B Cells Is Reduced in the Memory Compartment through Somatic Mutation of Nondominant Clones

Tian

Luskin

Dischert

et al. 2008

The Journal of Immunology

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Section: Discussionmentioning

confidence: 98%

mentioning

confidence: 99%

Immunodominance of the VH1–46 Antibody Gene Segment in the Primary Repertoire of Human Rotavirus-Specific B Cells Is Reduced in the Memory Compartment through Somatic Mutation of Nondominant Clones

Tian

Luskin

Dischert

et al. 2008

The Journal of Immunology

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“…Switched CD19 ϩ CD27 ϩ IgM Ϫ IgD Ϫ B cells are involved in T cell-dependent immune responses, secrete IgG and IgA Abs, and maintain long-term serological memory. IgM memory CD19 ϩ CD27 ϩ IgM high IgD low CD21 high B cells are important in T cell-independent immune responses and secrete high-affinity IgM in the early phase of infection (17) to inhibit microbial replication in blood. This cell population is believed to be the circulating counterparts of splenic marginal zone B cells and responds to the 23-valent polysaccharide pneumococcal vaccine (18).…”

Section: Loss Of Discretementioning

confidence: 99%

Loss of Discrete Memory B Cell Subsets Is Associated with Impaired Immunization Responses in HIV-1 Infection and May Be a Risk Factor for Invasive Pneumococcal Disease

Hart

Steel

Clark

et al. 2007

The Journal of Immunology

153

154

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Invasive pneumococcal infection is an important cause of morbidity and mortality in HIV-1-infected individuals. B cells play an important role in maintaining serologic memory after infection. IgM memory B cells are significantly reduced in HIV-1-infected patients and their frequency is similar to that observed in other patient groups (splenectomized individuals and patients with primary Ab deficiency) who are also known to have an increased risk of invasive pneumococcal infection. Antiretroviral therapy does not restore marginal zone B cell percentages. Immunization with the 23-valent polysaccharide pneumococcal vaccine shows that HIV-1-infected patients have impaired total IgM and IgG pneumococcal vaccines compared with healthy controls. Loss of switched memory B cells was associated with impaired tetanus toxoid IgG vaccine responses. Results of this study demonstrate that defects in B cell memory subsets are associated with impaired humoral immune responses in HIV-1 patients who are receiving antiretroviral therapy and may be a contributory factor to the increased risk of invasive pneumococcal infection observed in HIV-1 infection.

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“…Subsets of memory B cells can be easily, but not exhaustively, identified by the different expression of surface IgD and CD27 (Shi et al 2003). In addition, memory B cells are characterized by the presence of somatic hypermutation in the variable gene sequences of the immunoglobulins (Klein et al 1998).…”

Section: Introductionmentioning

confidence: 99%

B cell immunosenescence: different features of naive and memory B cells in elderly

et al. 2011

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Elderly people show a reduced protection against new infections and a decreased response to vaccines as a consequence of impairment of both cellular and humoral immunity. In this paper we have studied memory/naïve B cells in the elderly, evaluating surface immunoglobulin expression, production of the pro-and anti-inflammatory cytokines, tumor necrosis factor (TNF)-a and interleukin (IL)-10, and presence of somatic hypermutation, focusing on the IgG ? IgD -CD27 -double negative (DN) B cells that are expanded in the elderly. Our results show that naïve B cells from young donors need a sufficiently strong stimulus to be activated ''in vitro'', while naïve B cells from old subjects are able to produce IL-10 and TNF-a when stimulated ''physiologically'' (a-CD40/IL-4), suggesting that these cells might play a role in the control of the immuno-inflammatory environment in the elderly. In addition, in the elderly there is an accumulation of DN B cells with a reduced rate of somatic hypermutation. Thus, DN B lymphocytes may be exhausted cells that are expanded and accumulate as a by-product of persistent stimulation or impaired germinal center formation.

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Functional analysis of human memory B-cell subpopulations: IgD+CD27+ B cells are crucial in secondary immune response by producing high affinity IgM

Cited by 146 publications

References 44 publications

Immunodominance of the VH1–46 Antibody Gene Segment in the Primary Repertoire of Human Rotavirus-Specific B Cells Is Reduced in the Memory Compartment through Somatic Mutation of Nondominant Clones

Immunodominance of the VH1–46 Antibody Gene Segment in the Primary Repertoire of Human Rotavirus-Specific B Cells Is Reduced in the Memory Compartment through Somatic Mutation of Nondominant Clones

Loss of Discrete Memory B Cell Subsets Is Associated with Impaired Immunization Responses in HIV-1 Infection and May Be a Risk Factor for Invasive Pneumococcal Disease

B cell immunosenescence: different features of naive and memory B cells in elderly

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