Functional Analysis of Isoforms of NADPH:Protochlorophyllide Oxidoreductase (POR), PORB and PORC, in Arabidopsis thaliana

Masuda, Tatsuru; Fusada, Naoki; Oosawa, Naoki; Takamatsu, Ken’ichi; Yamamoto, Yoshiharu Y.; Ohto, Masa‐aki; Nakamura, Kenzo; Goto, Koji; Shibata, Daisuke; Shirano, Yumiko; Hayashi, Hiroaki; Kato, Tomohiko; Tabata, Satoshi; Shimada, Hiroshi; Ohta, Hiroyuki; Takamiya, Ken-ichiro

doi:10.1093/pcp/pcg128

Cited by 119 publications

(120 citation statements)

References 41 publications

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“…We selected the porB mutants because they represent the isoform that is constantly expressed in darkness and light, while PORC is light-dependently expressed and PORA is expressed only in etiolated tissue (Masuda et al, 2003). Interestingly, porB mutants actually express higher levels of LIL3 than the wild type.…”

Section: Altered Levels Of Chl Synthesis Proteins In Porbdeficient Mumentioning

confidence: 99%

LIL3, a Light-Harvesting Complex Protein, Links Terpenoid and Tetrapyrrole Biosynthesis in Arabidopsis thaliana

et al. 2017

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The LIL3 protein of Arabidopsis (Arabidopsis thaliana) belongs to the light-harvesting complex (LHC) protein family, which also includes the light-harvesting chlorophyll-binding proteins of photosystems I and II, the early-light-inducible proteins, PsbS involved in nonphotochemical quenching, and the one-helix proteins and their cyanobacterial homologs designated high-light-inducible proteins. Each member of this family is characterized by one or two LHC transmembrane domains (referred to as the LHC motif) to which potential functions such as chlorophyll binding, protein interaction, and integration of interacting partners into the plastid membranes have been attributed. Initially, LIL3 was shown to interact with geranylgeranyl reductase (CHLP), an enzyme of terpene biosynthesis that supplies the hydrocarbon chain for chlorophyll and tocopherol. Here, we show another function of LIL3 for the stability of protochlorophyllide oxidoreductase (POR). Multiple protein-protein interaction analyses suggest the direct physical interaction of LIL3 with POR but not with chlorophyll synthase. Consistently, LIL3-deficient plants exhibit substantial loss of POR as well as CHLP, which is not due to defective transcription of the POR and CHLP genes but to the posttranslational modification of their protein products. Interestingly, in vitro biochemical analyses provide novel evidence that LIL3 shows high binding affinity to protochlorophyllide, the substrate of POR. Taken together, this study suggests a critical role for LIL3 in the organization of later steps in chlorophyll biosynthesis. We suggest that LIL3 associates with POR and CHLP and thus contributes to the supply of the two metabolites, chlorophyllide and phytyl pyrophosphate, required for the final step in chlorophyll a synthesis.

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Section: Altered Levels Of Chl Synthesis Proteins In Porbdeficient Mumentioning

confidence: 99%

LIL3, a Light-Harvesting Complex Protein, Links Terpenoid and Tetrapyrrole Biosynthesis in Arabidopsis thaliana

et al. 2017

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“…These include phosphoglycerate kinase (AT1G56190), Fru-bisP aldolase (AT2G21330) Fru-1,6-bisphosphatase (AT3G54050), sedoheptulose-1,7-bisphosphatase (AT3G55800), phosphoribulokinase (AT1G32060), phosphoglycolate phosphatase (AT5G36700), Gly cleavage system H protein (AT1G32470), Gly cleavage system H protein 1 (AT2G35370), and carbonic anhydrases (AT3G52720, AT4G33580, and CA1). Furthermore, there is a repression of genes for enzymes that mediate key steps in the biosynthesis of photosynthetic pigments, such as chlorophyll (PORB and PORC; 5-to 10-fold) and carotenoids (PSY and GGPS; 3-and 2-fold, respectively); PORB and PORC are crucial for the photoconversion of protochlorophyllide to chlorophyllide, which is then converted to chlorophyll (Masuda et al, 2003). Likewise, PSY is the key regulatory step of carotenogenesis in plastids (Hirschberg, 2001).…”

Section: Photosynthesismentioning

confidence: 99%

Chloroplast Photooxidation-Induced Transcriptome Reprogramming in ArabidopsisimmutansWhite Leaf Sectors

et al. 2009

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Arabidopsis (Arabidopsis thaliana) immutans (im) has green and white sectoring due to the action of a nuclear recessive gene, IMMUTANS. The green sectors contain normal-appearing chloroplasts, whereas the white sectors contain abnormal chloroplasts that lack colored carotenoids due to a defect in phytoene desaturase activity. Previous biochemical and molecular characterizations of the green leaf sectors revealed alterations suggestive of a source-sink relationship between the green and white sectors of im. In this study, we use an Affymetrix ATH1 oligoarray to further explore the nature of sink metabolism in im white tissues. We show that lack of colored carotenoids in the im white tissues elicits a differential response from a large number of genes involved in various cellular processes and stress responses. Gene expression patterns correlate with the repression of photosynthesis and photosynthesis-related processes in im white tissues, with an induction of Suc catabolism and transport, and with mitochondrial electron transport and fermentation. These results suggest that energy is derived via aerobic and anaerobic metabolism of imported sugar in im white tissues for growth and development. We also show that oxidative stress responses are largely induced in im white tissues; however, im green sectors develop additional energy-dissipating mechanisms that perhaps allow for the formation of green sectors. Furthermore, a comparison of the transcriptomes of im white and norflurazon-treated white leaf tissues reveals global as well as tissue-specific responses to photooxidation. We conclude that the differences in the mechanism of phytoene desaturase inhibition play an important role in differentiating these two white tissues.

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“…The proteins encoded by these two genes are known to be the major components of prolamellar bodies in etioplasts of dark-grown seedlings (Masuda et al, 2003). It has been reported that both mRNAs are accumulated in etiolated seedlings but only PORB mRNA continues to accumulate in light-grown plants, while PORA mRNA rapidly disappeared after illumination.…”

Section: Characteristics Of the C2 And C3 Clustersmentioning

confidence: 99%

Gene Expression Profiling of the Tetrapyrrole Metabolic Pathway in Arabidopsis with a Mini-Array System

et al. 2004

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Tetrapyrrole compounds, such as chlorophylls, hemes, and phycobilins, are synthesized in many enzymatic steps. For regulation of the tetrapyrrole metabolic pathway, it is generally considered that several specific isoforms catalyzing particular enzymatic steps control the flow of tetrapyrrole intermediates by differential regulation of gene expression depending on environmental and developmental factors. However, the coordination of such regulatory steps and orchestration of the overall tetrapyrrole metabolic pathway are still poorly understood. In this study, we developed an original mini-array system, which enables the expression profiling of each gene involved in tetrapyrrole biosynthesis simultaneously with high sensitivity. With this system, we performed a transcriptome analysis of Arabidopsis seedlings in terms of the onset of greening, endogenous rhythm, and developmental control. Data presented here clearly showed that based on their expression profiles at the onset of greening, genes involved in tetrapyrrole biosynthesis can be classified into four categories, in which genes are coordinately regulated to control the biosynthesis. Moreover, genes in the same group were similarly controlled in an endogenous rhythmic manner but also by a developmental program. The physiological significance of these gene clusters is discussed.

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Functional Analysis of Isoforms of NADPH:Protochlorophyllide Oxidoreductase (POR), PORB and PORC, in Arabidopsis thaliana

Cited by 119 publications

References 41 publications

LIL3, a Light-Harvesting Complex Protein, Links Terpenoid and Tetrapyrrole Biosynthesis in Arabidopsis thaliana

LIL3, a Light-Harvesting Complex Protein, Links Terpenoid and Tetrapyrrole Biosynthesis in Arabidopsis thaliana

Chloroplast Photooxidation-Induced Transcriptome Reprogramming in ArabidopsisimmutansWhite Leaf Sectors

Gene Expression Profiling of the Tetrapyrrole Metabolic Pathway in Arabidopsis with a Mini-Array System

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