Functional analysis of Pogostemon cablin farnesyl pyrophosphate synthase gene and its binding transcription factor PcWRKY44 in regulating biosynthesis of patchouli alcohol

Wang, Xiaobing; Tang, Yun; Huang, Huiling; Wu, Daidi; Chen, Xiuzhen; Li, Junren; Hai, Zheng; Zhan, Renya; Chen, Likai

doi:10.3389/fpls.2022.946629

Cited by 7 publications

(11 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, BBS belongs to sesquiterpene synthases that are localized in the cytosol. Our results are consistent with previous reports, such as A. thaliana [ 21 , 22 , 23 ], periwinkle [ 24 ], and Aquilegia species [ 25 ]. In vitro enzyme activity analysis suggested that MrFPS and MrBBS act as a functional FPS and α-bisabolol synthase, respectively.…”

Section: Discussionsupporting

confidence: 94%

Biosynthesis of α-Bisabolol by Farnesyl Diphosphate Synthase and α-Bisabolol Synthase and Their Related Transcription Factors in Matricaria recutita L.

Tai

Wang

Yao

et al. 2023

IJMS

View full text Add to dashboard Cite

The essential oil of German chamomile (Matricaria recutita L.) is widely used in food, cosmetics, and the pharmaceutical industry. α-Bisabolol is the main active substance in German chamomile. Farnesyl diphosphate synthase (FPS) and α-bisabolol synthase (BBS) are key enzymes related to the α-bisabolol biosynthesis pathway. However, little is known about the α-bisabolol biosynthesis pathway in German chamomile, especially the transcription factors (TFs) related to the regulation of α-bisabolol synthesis. In this study, we identified MrFPS and MrBBS and investigated their functions by prokaryotic expression and expression in hairy root cells of German chamomile. The results suggest that MrFPS is the key enzyme in the production of sesquiterpenoids, and MrBBS catalyzes the reaction that produces α-bisabolol. Subcellular localization analysis showed that both MrFPS and MrBBS proteins were located in the cytosol. The expression levels of both MrFPS and MrBBS were highest in the extension period of ray florets. Furthermore, we cloned and analyzed the promoters of MrFPS and MrBBS. A large number of cis-acting elements related to light responsiveness, hormone response elements, and cis-regulatory elements that serve as putative binding sites for specific TFs in response to various biotic and abiotic stresses were identified. We identified and studied TFs related to MrFPS and MrBBS, including WRKY, AP2, and MYB. Our findings reveal the biosynthesis and regulation of α-bisabolol in German chamomile and provide novel insights for the production of α-bisabolol using synthetic biology methods.

show abstract

Section: Discussionsupporting

confidence: 94%

Biosynthesis of α-Bisabolol by Farnesyl Diphosphate Synthase and α-Bisabolol Synthase and Their Related Transcription Factors in Matricaria recutita L.

Tai

Wang

Yao

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…34 Heterologous overexpression of transgenic PcFPPS in yeast cells and N. benthamiana significantly increased the content of terpenoids and promoted the biosynthesis of patchouli alcohol in synergy with WRKY44. 23 GpFPS1 and GpMYB81 synergistically promote the biosynthesis of gypenosides in Gynostemma pentaphyllum. 35 It can be seen that the FPS gene plays a key regulatory role in the secondary metabolism of various plants and promotes the accumulation of triterpenoids and the high yield of active ingredients.…”

Section: Discussionmentioning

confidence: 97%

“…16 To date, FPS has been cloned and characterized in a variety of plants, and it has been found that FPS1 and FPS2 genes play key roles during embryonic development in seeds of A. thaliana, and the LeFPS1 gene is enriched in young leaves of tomato, which is involved in the metabolism of farnesyl pyrophosphate, affecting cell division and enlargement. 17,18 In addition, the FPS genes in P. ginseng, 19 Artemisia annua, 20 Poria cocos, 21 Tripterygium wilfordii, 22 Pogostemon cablin, 23 Dendrobium nobile, 24 and B. platyphylla 25 were also cloned and verified.…”

Section: Introductionmentioning

confidence: 99%

Cloning, Expression Characteristics of Farnesyl Pyrophosphate Synthase Gene from Platycodon grandiflorus and Functional Identification in Triterpenoid Synthesis

Liu,

Wang,

Qin

et al. 2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

Platycodon grandiflorus is a medicinal plant whose main component is platycodins, which have a variety of pharmacological effects and nutritional values. The farnesyl pyrophosphate synthase (FPS) is a key enzyme in the isoprenoid biosynthesis pathway, which catalyzes the synthesis of farnesyl diphosphate (FPP). In this study, we cloned the FPS gene from P. grandiflorus (PgFPS) with an ORF of 1260 bp, encoding 419 amino acids with a deduced molecular weight and theoretical pI of 46,200.98 Da and 6.52, respectively. The squalene content of overexpressed PgFPS in tobacco leaves and yeast cells extract was 1.88-fold and 1.21-fold higher than that of the control group, respectively, and the total saponin content was also increased by 1.15 times in yeast cells extract, which verified the biological function of PgFPS in terpenoid synthesis. After 48 h of MeJA treatment and 6 h of ethephon treatment, the expression of the PgFPS gene in roots and stems reached its peak, showing a 3.125-fold and 3.236-fold increase compared to the untreated group, respectively. Interestingly, the expression of the PgFPS gene in leaves showed a decreasing trend after exogenous elicitors treatment. The discovery of this enzyme will provide a novel perspective for enhancing the efficient synthesis of platycodins.

show abstract

“…Neophytadiene and phytol are diterpenoids, and little research has been done on their biosynthesis. In our previous study, OEL4 and OEL12 transgenic tobacco plants, which overexpressed PcFPPS, exhibited significantly higher levels of phytol and neophytadiene than wild-type lines (Wang et al, 2022). Based on the synthetic pathways involved in terpenoid biosynthesis, diterpenoid biosynthesis is widely thought to be related to MEP synthesis.…”

Section: )mentioning

confidence: 99%

GC-MS and UHPLC-QTOFMS-assisted identification of the differential metabolites and metabolic pathways in key tissues of Pogostemon cablin

et al. 2023

Self Cite

View full text Add to dashboard Cite

Pogostemon cablin is an important aromatic medicinal herb widely used in the pharmaceutical and perfume industries. However, our understanding of the phytochemical compounds and metabolites within P. cablin remains limited. To our knowledge, no integrated studies have hitherto been conducted on the metabolites of the aerial parts of P. cablin. In this study, twenty-three volatile compounds from the aerial parts of P. cablin were identified by GC-MS, predominantly sesquiterpenes. Quantitative analysis showed the highest level of patchouli alcohol in leaves (24.89 mg/g), which was 9.12 and 6.69-fold higher than in stems and flowers. UHPLC-QTOFMS was used to analyze the non-volatile compounds of leaf, stem and flower tissues. The differences in metabolites between flower and leaf tissues were the largest. Based on 112, 77 and 83 differential metabolites between flower-leaf, flower-stem and leaf-stem, three tissue-specific biomarkers of metabolites were identified, and the differential metabolites were enriched in several KEGG pathways. Furthermore, labeling differential metabolites in the primary and secondary metabolic pathways showed that flowers accumulated more lipids and amino acids, including proline, lysine and tryptophan; the leaves accumulated higher levels of terpenoids, vitamins and flavonoids, and stems contained higher levels of carbohydrate compounds. Based on the role of acetyl coenzyme A, the distribution and possible exchange mechanism of metabolites in leaves, stems and flowers of P. cablin were mapped for the first time, laying the groundwork for future research on the metabolites in P. cablin and their regulatory role.

show abstract

Functional analysis of Pogostemon cablin farnesyl pyrophosphate synthase gene and its binding transcription factor PcWRKY44 in regulating biosynthesis of patchouli alcohol

Cited by 7 publications

References 43 publications

Biosynthesis of α-Bisabolol by Farnesyl Diphosphate Synthase and α-Bisabolol Synthase and Their Related Transcription Factors in Matricaria recutita L.

Biosynthesis of α-Bisabolol by Farnesyl Diphosphate Synthase and α-Bisabolol Synthase and Their Related Transcription Factors in Matricaria recutita L.

Cloning, Expression Characteristics of Farnesyl Pyrophosphate Synthase Gene from Platycodon grandiflorus and Functional Identification in Triterpenoid Synthesis

GC-MS and UHPLC-QTOFMS-assisted identification of the differential metabolites and metabolic pathways in key tissues of Pogostemon cablin

Contact Info

Product

Resources

About